Crystallization and preliminary X-ray analysis of a proteinase-K-resistant domain within the phosphoprotein of vesicular stomatitis virus (Indiana)

Academic Article

Abstract

  • Several stable domains of the phosphoprotein (P) of vesicular stomatitis virus (Indiana) were identified by limited proteolysis of purified recombinant P protein expressed in Escherichia coli. The proteinase-K-resistant domain could be crystallized using ammonium sulfate as a precipitant and ethylene glycol as an additive. The crystals belong to space group P4 2 2 or P4 2 2, with unit-cell parameters a = b = 74.50, c = 156.84 Å. X-ray diffraction data were collected to 2.75 Å resolution at a synchrotron-radiation source. © 2004 International Union of Crystallography. 1 1 3 1
  • Authors

    Digital Object Identifier (doi)

    Author List

  • Ding H; Green TJ; Luo M
  • Start Page

  • 2087
  • End Page

  • 2090
  • Volume

  • 60
  • Issue

  • 11