Both RIG-I and MDA5 detect alphavirus replication in concentration-dependent mode

Academic Article

Abstract

  • © 2015 Elsevier Inc. Alphaviruses are a family of positive-strand RNA viruses that circulate on all continents between mosquito vectors and vertebrate hosts. Despite a significant public health threat, their biology is not sufficiently investigated, and the mechanisms of alphavirus replication and virus-host interaction are insufficiently understood. In this study, we have applied a variety of experimental systems to further understand the mechanism by which infected cells detect replicating alphaviruses. Our new data strongly suggest that activation of the antiviral response by alphavirus-infected cells is determined by the integrity of viral genes encoding proteins with nuclear functions, and by the presence of two cellular pattern recognition receptors (PRRs), RIG-I and MDA5. No type I IFN response is induced in their absence. The presence of either of these PRRs is sufficient for detecting virus replication. However, type I IFN activation in response to pathogenic alphaviruses depends on the basal levels of RIG-I or MDA5.
  • Published In

  • Virology  Journal
  • Digital Object Identifier (doi)

    Author List

  • Akhrymuk I; Frolov I; Frolova EI
  • Start Page

  • 230
  • End Page

  • 241
  • Volume

  • 487