Identification of a novel retroviral gene unique to human immunodeficiency virus type 2 and simian immunodeficiency virus SIV(MAC)

Academic Article

Abstract

  • Human and simian immunodeficiency-associated retroviruses are extraordinarily complex, containing at least five genes, tat, art, sor, R, and 3' orf, in addition to the structural genes gag, pol, and env. Recently, nucleotide sequence analysis of human immunodeficiency virus type 2 (HIV-2) and simian immunodeficiency virus SIV(MAC) revealed the existence of still another open reading frame, termed X, which is highly conserved between these two viruses but absent from HIV-1. In this report, we demonstrate for the first time that the X open reading frame represents a functional retroviral gene in both HIV-2 and SIV(MAC) and that it encodes a virion-associated protein of 14 and 12 kilodaltons, respectively. We also describe the production of recombinant TrpE/X fusion proteins in Escherichia coli and show that sera from some HIV-2-infected individuals specifically recognize these proteins.
  • Published In

    Digital Object Identifier (doi)

    Author List

  • Kappes JC; Morrow CD; Lee SW; Jameson BA; Kent SBH; Hood LE; Shaw GM; Hahn BH
  • Start Page

  • 3501
  • End Page

  • 3505
  • Volume

  • 62
  • Issue

  • 9