Phospholipase C γ1 regulates early secretory trajficking and cell migration via interaction with p115

Academic Article


  • The role of early secretory trajficking in the regulation of cell motility remains incompletely understood. Here we used a small interfering RNA screen to monitor the effects on structure of the Golgi apparatus and cell migration. Two major Golgi phenotypes were observed - fragmented and small Golgi. The latter exhibited a stronger correlation with a defect in cell migration. Among the small Golgi hits, we focused on phospholipase C γ1 (PLCγ1). We show that PLCγ1 regulates Golgi structure and cell migration independently of its catalytic activity but in a manner that depends on interaction with the tethering protein p115. PLCγ1 regulates the dynamics of p115 in the early secretory pathway, thereby controlling trajficking from the endoplasmic reticulum to the Golgi. Our results uncover a new function of PLCγ1 that is independent of its catalytic function and link early secretory trajficking to the regulation of cell migration.
  • Published In

    Digital Object Identifier (doi)

    Author List

  • Millarte V; Boncompain G; Tillmann K; Perez F; Sztul E; Farhan H
  • Start Page

  • 2263
  • End Page

  • 2278
  • Volume

  • 26
  • Issue

  • 12