An Alternative Procedure for Isolation of Rainbox Trout, Oncorhynchus mykiss, Serum Immunoglobulin

Academic Article

Abstract

  • A method for purification of rainbow trout, Oncorhynchus mykiss, serum immunoglobulin (Ig) that relies on specific retention of rainbow trout Ig in the presence of other serum proteins, by an ion-exchange chromatography matrix (ABx resin) previously developed for the isolation of mammalian Ig is described. This protocol allows for rapid and substantial purification of total Ig including antigen-specific antibodies from the serum of rainbow trout. The procedure does not utilize antibody-derivatized matrices for immunoabsorption so it is suited to situations where fish Ig-specific antisera or monoclonal antibody is unavailable. Furthermore, total Ig can be isolated from pre-immune sera since no antigen-derivatized affinity matrices are used, eliminating the requirement for lengthy immunization regimens. With minor modification, ABx-chromatography can be adapted for the isolation of Ig from other species of fish. © 1995 Taylor & Francis Group, LLC.
  • Published In

    Digital Object Identifier (doi)

    Author List

  • Szalai AJ; Banoub J; Nakhla NA; Keough KMW
  • Start Page

  • 33
  • End Page

  • 45
  • Volume

  • 5
  • Issue

  • 3