Genotypic and morphogenetic differences among three female varieties of P. longum, one variety each from Assam and Calicut and one released variety, Viswam, were investigated for the development of a common and efficient method of plant regeneration. RAPD analysis, using random oligonucleotide primers, revealed that these varieties are genetically different. Compared to the Assam variety, Viswam and Calicut varieties are genetically closer (95% similarity) among themselves. Morphogenetic potential of node, internode and leaf explants from all the three varieties were compared. Leaf explants from different varieties exhibited maximum regeneration potential. Among the types tested, Viswam variety exhibited best morphogenetic response followed by the varieties from Calicut and Assam. An efficient protocol was developed for regeneration from leaf calli of all the three genotypically different varieties. Callus regenerated plants from leaf explants were subsequently rooted, hardened and established on soil under natural conditions of growth.