Survival and integration rate of channel catfish and common carp embryos microinjected with DNA at various developmental stages

Academic Article

Abstract

  • Channel catfish, Ictalurus punctatus, and common carp, Cyprinus carpio, were microinjected with DNA at early, intermediate or late one-cell stage, two-cell stage or four-cell stage to determine the most appropriate developmental stage for maximizing the production of transgenic individuals, a product of survival × integration rate. Survival (hatching rate) decreased (P < 0.05, r = -0.81) when channel catfish embryos were microinjected at later stages compared to earlier stages. Survival relative to non-microinjected controls was 65.0, 43.6, 38.5, 27.4, and 35.9% and integration rate was 2.1, 1.5, 15.2, 12.5 and 7.7% for early one-cell, intermediate one-cell, late one-cell, two-cell and four-cell stages, respectively. Cell stage at time of microinjection did not affect (P > 0.05, r= -0.52) survival in common carp and survival of microinjected common carp embryos was higher (P < 0.01) than survival for channel catfish embryos. Survival relative to non-microinjected controls was 88.9, 85.3, 91.5, 85.3, and 83.4% and integration rate was 8.3, 1.0, 0.0, 11.5 and 17% for early one-cell, intermediate one-cell, late one-cell, two-cell and four-cell stages, respectively, of common carp. The late one-cell and the two-cell stage were the most appropriate stages for maximizing production of transgenic channel catfish and common carp, respectively. © 1991.
  • Published In

  • Aquaculture  Journal
  • Digital Object Identifier (doi)

    Author List

  • Hayat M; Joyce CP; Townes TM; Chen TT; Powers DA; Dunham RA
  • Start Page

  • 249
  • End Page

  • 255
  • Volume

  • 99
  • Issue

  • 3-4