Atomic model of a pyrimidine dimer excision repair enzyme complexed with a dna substrate: Structural basis for damaged DNA recognition

Academic Article

Abstract

  • T4 endonuclease V is a DNA repair enzyme from bacteriophage T4 that catalyzes the first reaction step of the pyrimidine dimer-specific base excision repair pathway. The crystal structure of this enzyme complexed with a duplex DNA substrate, containing a thymine dimer, has been determined at 2.75 Å resolution. The atomic structure of the complex reveals the unique conformation of the DNA duplex, which exhibits a sharp kink with a 60° inclination at the central thymine dimer. The adenine base complementary to the 5′ side of the thymine dimer is completely flipped out of the DNA duplex and trapped in a cavity on the protein surface. These structural features allow an understanding of the catalytic mechanism and implicate a general mechanism of how other repair enzymes recognize damaged DNA duplexes. © 1995.
  • Published In

  • Cell  Journal
  • Digital Object Identifier (doi)

    Author List

  • Vassylyev DG; Kashiwagi T; Mikami Y; Ariyoshi M; Iwai S; Ohtsuka E; Morikawa K
  • Start Page

  • 773
  • End Page

  • 782
  • Volume

  • 83
  • Issue

  • 5