Proton Nuclear Magnetic Resonance Studies on the Variant-3 Neurotoxin from Centruroides sculpturatus Ewing: Sequential Assignment of Resonances

Academic Article

Abstract

  • We report the sequential assignment of resonances to specific residues in the proton nuclear magnetic resonance spectrum of the variant-3 neurotoxin from the scorpion Centruroides sculpturatus Ewing (range southwestern U.S.A.). A combination of two-dimensional NMR experiments such as 2D-COSY, 2D-NOESY, and single- and double-RELAY coherence transfer spectroscopy has been employed on samples of the protein dissolved in D2O and in H2O for assignment purposes. These studies provide a basis for the determination of the solution-phase conformation of this protein and for undertaking detailed structure-function studies of these neurotoxins that modulate the flow of sodium current by binding to the sodium channels of excitable membranes. © 1989, American Chemical Society. All rights reserved.
  • Authors

    Published In

  • Biochemistry  Journal
  • Digital Object Identifier (doi)

    Author List

  • Nettesheim DG; Klevit RE; Drobny G; Watt DD; Krishna NR
  • Start Page

  • 1548
  • End Page

  • 1555
  • Volume

  • 28
  • Issue

  • 4