Pex35 is a regulator of peroxisome abundance

Academic Article

Abstract

  • Peroxisomes are cellular organelles with vital functions in lipid, amino acid and redox metabolism. The cellular formation and dynamics of peroxisomes are governed by PEX genes; however, the regulation of peroxisome abundance is still poorly understood. Here, we use a high-content microscopy screen in Saccharomyces cerevisiae to identify new regulators of peroxisome size and abundance. Our screen led to the identification of a previously uncharacterized gene, which we term PEX35, which affects peroxisome abundance. PEX35 encodes a peroxisomal membrane protein, a remote homolog to several curvature-generating human proteins. We systematically characterized the genetic and physical interactome as well as the metabolome of mutants in PEX35, and we found that Pex35 functionally interacts with the vesicle-budding-inducer Arf1. Our results highlight the functional interaction between peroxisomes and the secretory pathway.
  • Published In

    Digital Object Identifier (doi)

    Author List

  • Yofe I; Soliman K; Chuartzman SG; Morgan B; Weill U; Yifrach E; Dick TP; Cooper SJ; Ejsing CS; Schuldiner M
  • Start Page

  • 791
  • End Page

  • 804
  • Volume

  • 130
  • Issue

  • 4