Vectorology and factor delivery in induced pluripotent stem cell reprogramming

Academic Article

Abstract

  • Induced pluripotent stem cell (iPSC) reprogramming requires sustained expression of multiple reprogramming factors for a limited period of time (10-30 days). Conventional iPSC reprogramming was achieved using lentiviral or simple retroviral vectors. Retroviral reprogramming has flaws of insertional mutagenesis, uncontrolled silencing, residual expression and re-activation of transgenes, and immunogenicity. To overcome these issues, various technologies were explored, including adenoviral vectors, protein transduction, RNA transfection, minicircle DNA, excisable PiggyBac (PB) transposon, Cre-lox excision system, negative-sense RNA replicon, positive-sense RNA replicon, Epstein-Barr virus-based episomal plasmids, and repeated transfections of plasmids. This review provides summaries of the main vectorologies and factor delivery systems used in current reprogramming protocols. © Copyright 2014, Mary Ann Liebert, Inc. 2014.
  • Authors

    Digital Object Identifier (doi)

    Author List

  • Hu K
  • Start Page

  • 1301
  • End Page

  • 1315
  • Volume

  • 23
  • Issue

  • 12