Cathepsin E is a specific marker of dysplasia in APC Min/+ mouse intestine

Academic Article

Abstract

  • Transcriptional profiling of APC Min/+ mouse intestinal epithelial tissue has revealed that cathepsin E (catE) manifests high relative expression in adenomas and carcinomas relative to normal epithelium. Real-time RT-PCR data presented previously confirm the presence of catE transcript in APC Min/+ adenomatous cells compared with samples derived from normal APC Min/+ and wild-type tissue. At the protein level, strong, highly specific immunohistochemical staining for catE is displayed in dysplastic lesions of APC Min/+ mice. Using Western immunoblot analyses, it was additionally established that the urine of tumor-bearing mice contains higher levels of the monomeric form of catE than their wild-type counterparts. These results authenticate the relationship between transcript abundance and protein levels in transformed tissue and suggest potential utility for catE as a marker for the inception and progression of intestinal cancers. Copyright © 2006 S. Karger AG.
  • Published In

  • Tumor Biology  Journal
  • Digital Object Identifier (doi)

    Author List

  • Busquets L; Guillen H; DeFord ME; Suckow MA; Navari RM; Castellino FJ; Prorok M
  • Start Page

  • 36
  • End Page

  • 42
  • Volume

  • 27
  • Issue

  • 1