Validation of Dual-energy X-ray Absorptiometry to Predict Body Composition of Channel Catfish, Ictalurus punctatus

Academic Article


  • Dual-energy X-ray absorptiometry (DXA) provides a noninvasive way to determine lean tissue mass (LTM), fat mass (FM), bone mineral content (BMC), and bone mineral density (BMD) in humans and small mammals. Live channel catfish (n = 74, 78–1200 g) were anesthetized and scanned in both a lateral position and a dorsa-ventral position. Six individual fish (300–600 g) were scanned five times each to determine precision by the coefficient of variation. Precision was good for LTM (0.75–1.06%) and for BMC and BMD (2–2.6%). Precision for FM was not good (27–34%), which was due to the very low FM (0–1 g) recorded by the DXA. However, using the predicted values, FM precision improved to 5–5.5%. DXA values for LTM, FM, and BMC were significantly different from chemical analysis (P < 0.001). DXA overestimated LTM and underestimated FM and BMC. However, all three compartments were strongly correlated with carcass values (P < 0.0001). Using the prediction equations and the jackknife procedure, predicted values of LTM, FM, and BMC were not significantly different from the carcass values (P > 0.05). DXA may also be a valuable tool for evaluating body condition longitudinally in commercial or in threatened or endangered fish species, where noninvasive procedures would be invaluable.
  • Authors

    Digital Object Identifier (doi)

    Author List

  • Johnson MS; Watts RJ; Hammer HS; Nagy TR; Watts SA
  • Start Page

  • 122
  • End Page

  • 131
  • Volume

  • 48
  • Issue

  • 1