Selected Publications

Academic Article

Year Title Altmetric
2019 Effect of incubation with crystalloid solutions or medications on packed red blood cellsTransfusion.  59:2643-2651. 2019
2018 Potential modulation of vascular function by nitric oxide and reactive oxygen species released from erythrocytesFrontiers in Physiology.  9. 2018
2018 Research Fellow, US Army Medical Research Institute for Chemical Defense,Frontiers in Physiology.  9. 2018
2016 Ferriheme catalyzes nitric oxide reaction with glutathione to form S-nitrosoglutathione: A novel mechanism for formation of S-nitrosothiolsFree Radical Biology and Medicine.  101:296-304. 2016
2016 The Impact of Surgery and Stored Red Blood Cell Transfusions on Nitric Oxide HomeostasisAnesthesia and Analgesia.  123:274-282. 2016
2016 Red blood cells stored 35 days or more are associated with adverse outcomes in high-risk patientsTransfusion.  56:1690-1698. 2016
2016 Oxidative stress and rheologic properties of stored red blood cells before and after transfusion to surgical patientsTransfusion.  56:1101-1111. 2016
2016 2,3-Diphosphoglycerate Concentrations in Autologous Salvaged Versus Stored Red Blood Cells and in Surgical Patients after TransfusionAnesthesia and Analgesia.  122:616-623. 2016
2015 Red blood cell membrane-facilitated release of nitrite-derived nitric oxide bioactivityBiochemistry.  54:6712-6723. 2015
2015 The pathophysiology of extracellular hemoglobin associated with enhanced oxidative reactionsFrontiers in Physiology.  6. 2015
2014 The effect of intermittent pneumatic compression of legs on the levels of nitric oxide related species in blood and on arterial function in the armNitric Oxide: Biology and Chemistry.  40:117-122. 2014
2014 New insights provided by a comparison of impaired deformability with erythrocyte oxidative stress for sickle cell diseaseBlood Cells, Molecules and Diseases.  52:230-235. 2014
2014 Red blood cell oxidative stress impairs oxygen delivery and induces red blood cell agingFrontiers in Physiology.  5 FEB. 2014
2013 Routes for Formation of S-Nitrosothiols in BloodCell Biochemistry and Biophysics.  67:385-398. 2013
2013 Hemoglobin redox reactions and red blood cell agingAntioxidants and Redox Signaling.  18:2274-2283. 2013
2013 SOD2 deficiency in hematopoietic cells in mice results in reduced red blood cell deformability and increased heme degradationExperimental Hematology.  41:316-321. 2013
2013 Role of peroxiredoxin-2 in protecting RBCs from hydrogen peroxide-induced oxidative stress 2013
2012 Racial discrimination is associated with a measure of red blood cell oxidative stress: A potential pathway for racial health disparitiesInternational Journal of Behavioral Medicine.  19:489-495. 2012
2011 The quaternary hemoglobin conformation regulates the formation of the nitrite-induced bioactive intermediate and the dissociation of nitric oxide from this intermediateNitric Oxide: Biology and Chemistry.  24:102-109. 2011
2011 Determination of S-NitrosothiolsS-Nitrosothiols in Biological Fluids by ChemiluminescenceChemiluminescenceMethods in Molecular Biology.  704:27-37. 2011
2010 Assessment of antioxidant activity of eugenol in vitro and in vivo.Methods in Molecular Biology.  610:165-180. 2010
2010 Measurement of plasma nitrite by chemiluminescence.Methods in Molecular Biology.  610:41-49. 2010
2010 Role of the membrane in the formation of heme degradation products in red blood cellsLife Sciences.  86:133-138. 2010
2009 Nitrite-induced improved blood circulation associated with an increase in a pool of RBC-NO with NO bioactivityAdvances in Experimental Medicine and Biology.  645:27-34. 2009
2009 Nitrite enhances RBC hypoxic ATP synthesis and the release of ATP into the vasculature: A new mechanism for nitrite-induced vasodilation 2009
2009 Quantification of intermediates formed during the reduction of nitrite by deoxyhemoglobinJournal of Biological Chemistry.  284:12710-12718. 2009
2009 Vascular endothelial barrier dysfunction mediated by amyloid-β proteinsJournal of Alzheimer's Disease.  17:845-854. 2009
2008 Iron-deficiency anaemia enhances red blood cell oxidative stress 2008
2008 Heme degradation and oxidative stress in murine models for hemoglobinopathies: Thalassemia, sickle cell disease and hemoglobin C diseaseBlood Cells, Molecules and Diseases.  41:60-66. 2008
2008 Red blood cells induce hypoxic lung inflammationBlood.  111:5205-5214. 2008
2007 Intermediates detected by visible spectroscopy during the reaction of nitrite with deoxyhemoglobin: The effect of nitrite concentration and diphosphoglycerateBiochemistry.  46:11650-11659. 2007
2007 Nitrite infusion increases cerebral blood flow and decreases mean arterial blood pressure in rats: A role for red cell NONitric Oxide: Biology and Chemistry.  16:448-456. 2007
2007 Measurement of plasma nitrite by chemiluminescence without interference of S-, N-nitroso and nitrated speciesFree Radical Biology and Medicine.  42:1146-1154. 2007
2006 S-Nitrosohemoglobin: A mechanism for its formation in conjunction with nitrite reduction by deoxyhemoglobinNitric Oxide: Biology and Chemistry.  15:20-29. 2006
2006 Nitric oxide redox reactions and red cell biologyAntioxidants and Redox Signaling.  8:1193-1203. 2006
2005 Red cell interactions with amyloid-β1-40 fibrils in a murine modelNeurobiology of Disease.  19:28-37. 2005
2004 Heme degradation by reactive oxygen speciesAntioxidants and Redox Signaling.  6:967-978. 2004
2004 Influence of beta-amyloid fibrils on the interactions between red blood cells and endothelial cellsNeurological Research.  26:579-585. 2004
2004 Redox Reactions of HemoglobinAntioxidants and Redox Signaling.  6:657-666. 2004
2003 Hemoglobin redox reactions and oxidatives stressRedox Report.  8:234-237. 2003
2003 Active Nitric Oxide Produced in the Red Cell under Hypoxic Conditions by Deoxyhemoglobin-mediated Nitrite ReductionJournal of Biological Chemistry.  278:46349-46356. 2003
2003 Red cell perturbations by amyloid β-protein 2003
2003 Hydrogen-peroxide-induced heme degradation in red blood cells: The protective roles of catalase and glutathione peroxidase 2003
2002 Site-specific cross-linking of human and bovine hemoglobins differentially alters oxygen binding and redox side reactions producing rhombic heme and heme degradationBiochemistry.  41:7407-7415. 2002
2000 The origin of red cell fluorescence caused by hydrogen peroxide treatmentFree Radical Biology and Medicine.  29:659-663. 2000
2000 Reaction of hydrogen peroxide with ferrylhemoglobin: Superoxide production and heme degradationBiochemistry.  39:12503-12511. 2000
2000 Heme degradation during autoxidation of oxyhemoglobinBiochemical and Biophysical Research Communications.  273:839-845. 2000
1998 Superoxide produced in the heme pocket of the β-chain of hemoglobin reacts with the β-93 cysteine to produce a thiyl radicalBiochemistry.  37:13194-13202. 1998
1998 Formation of fluorescent heme degradation products during the oxidation of hemoglobin by hydrogen peroxideBiochemical and Biophysical Research Communications.  247:592-596. 1998
1997 Hydrogen peroxide produced during autoxidation associated with heme degradation 1997
1997 Inhibition of xanthine oxidase-xanthine-iron mediated lipid peroxidation by eugenol in liposomes 1997
1996 Inhibition of lipoxygenase-mediated lipid peroxidation by eugenol in a liposomal systemJournal of Clinical Biochemistry and Nutrition.  21:123-129. 1996
1995 The protective effects of eugenol on carbon tetrachloride induced hepatotoxicity in rats 1995
1994 Inhibition of microsomal lipid peroxidation and monooxygenase activities by eugenol 1994
1992 Inhibitory effect of eugenol on non-enzymatic lipid peroxidation in rat liver mitochondriaBiochemical Pharmacology.  43:2393-2400. 1992


Year Title Altmetric
2002 Aging and the red cell.  Ed. 11.  2002

Research Overview

  • Decreased bioavailability of nitric oxide and subsequent dysregulation of nitric oxide signaling is a major factor for the development of cardiopulmonary vascular diseases. My studies are focused on developing a technology to increase the bioavailability of nitric oxide levels using exogenous source of nitric oxide donors such as low molecular weight S-nitrosothiols (nitric oxide adducts to thiols) and inorganic nitrite in experimental animal models and human clinical studies. My studies also focused on developing inhaled gaseous and aerosolized drug delivery systems to treat highly debilitating pulmonary hypertension diseases.
  • Teaching Overview


    2015 Joshua A. Lipsitz, , Undergraduate student
    Medical Student, George Washington University School of Medicine, Washington DC

    2015 - 2016 Andrew V. Scott, Medical student
    Resident, Anesthesiology The Johns Hopkins Hospital, Baltimore, MD

    2016 - Daniel J. Johnson, Medical student

    2016 - 2017 Ian M. Dwyer, Undergraduate student
    Medical Student, Albert Einstein College of Medicine, New York City, NY

    2018 Aishwarya Sriraman, Graduate student
    Research Fellow, US Army Medical Research Institute for Chemical Defense,
    ORISE, Raleigh, NC
  • Education And Training

  • National Institutes of Health National Institute of Aging, Postdoctoral Fellowship
  • Doctor of Philosophy Level Degree in Biochemistry, Osmania University 1995
  • Full Name

  • Nagababu Enika