Positions

Overview

  • Dr. Mobley is considered an expert in proteomics by his peers, and as director of the Mass Spectrometry/Proteomics Shared Facility, continues to focus on clinical and translational proteomics applications, which include statistical analysis of high density 'omics driven data sets, and the use of systems biology applications to uncover biologically relevant pathways. He has nearly 30 years experience in academia and industry, and mass spectrometry has been a intricate part of his work experience and studies from the beginning.

    Dr. Mobley obtained his B.S. in Chemistry at Oregon State University. He obtained a Ph.D. in Medicinal Chemistry and Pharmacology from The Ohio State University with an emphasis on hormonal induced carcinogesis. Prior to joining the faculty at UAB in 2006, Dr. Mobley worked as a faculty member at UMass Medical School and also at Vanderbilt.

    In 2007, Dr. Mobley became Director of the newly formed UAB Comprehensive Cancer Center Clinical Proteomics Shared Facility, and due to the success of that facility he was asked to expand in 2009 to encompass a single campus wide MS/ Proteomics Shared Facility (MSP-SF).
  • Selected Publications

    Academic Article

    Year Title Altmetric
    2020 14-3-3 proteins protect AMPK-phosphorylated ten-eleven translocation-2 (TET2) from PP2A-mediated dephosphorylation 2020
    2019 Oxylipins mediate cell-to-cell communication in Pseudomonas aeruginosa 2019
    2019 Affinity purification of NF1 protein–protein interactors identifies keratins and neurofibromin itself as binding partners 2019
    2019 Proteomic analysis of urinary extracellular vesicles reveal biomarkers for neurologic disease 2019
    2019 Multi-Omics Profiling for NF1 Target Discovery in Neurofibromin (NF1) Deficient Cells 2019
    2019 Myristoylated alanine-rich C-kinase substrate effector domain phosphorylation regulates the growth and radiation sensitization of glioblastoma 2019
    2019 Correction: Vascular endothelial tight junctions and barrier function are disrupted by 15(S)-hydroxyeicosatetraenoic acid partly via protein kinase C ?-mediated zona occludens-1 phosphorylation at threonine 770/772 (Journal of Biological Chemistry (2014) 289 (3148-3163) DPO: 10.1074/jbc.M113.528190) 2019
    2019 Fibronectin on the surface of extracellular vesicles mediates fibroblast invasion 2019
    2019 Erratum: 12/15-Lipoxygenase-dependent ROS production is required for diet-induced endothelial barrier dysfunction (Journal of Lipid Research (2015) 56 (562–577) DOI: 10.1194/jlr.M055566) 2019
    2018 Proteomic profiling of extracellular vesicles released from vascular smooth muscle cells during initiation of phosphate-induced mineralization 2018
    2018 Multiple host factors interact with the hypervariable domain of chikungunya virus nsP3 and determine viral replication in cell-specific mode 2018
    2018 Reactive species balance via GTP cyclohydrolase i regulates glioblastoma growth and tumor initiating cell maintenance 2018
    2018 Epitope mapping of Salmonella flagellar hook-associated protein, FlgK, with mass spectrometry-based immuno-capture proteomics using chicken (Gallus gallus domesticus) sera 2018
    2018 The retinoid X receptor agonist, 9-cis UAB30, inhibits cutaneous T-cell lymphoma proliferation through the SKP2-p27kip1 axis 2018
    2018 Chemotherapy induces secretion of exosomes loaded with heparanase that degrades extracellular matrix and impacts tumor and host cell behavior 2018
    2017 Identification of the amino acids inserted during suppression of CFTR nonsense mutations and determination of their functional consequences 2017
    2017 CD4 + T cells from HIV-1 patients with impaired Th1 effector responses to Mycobacterium tuberculosis exhibit diminished histone and nucleoprotein signatures 2017
    2017 Hypervariable domain of eastern equine encephalitis virus nsP3 redundantly utilizes multiple cellular proteins for replication complex assembly 2017
    2017 Endostatin inhibits androgen-independent prostate cancer growth by suppressing nuclear receptor-mediated oxidative stress 2017
    2017 Regulation of FcRγ function by site-specific serine phosphorylation 2017
    2016 Ataluren stimulates ribosomal selection of near-cognate tRNAs to promote nonsense suppression 2016
    2016 Serum protein profiling in diffuse large B-cell lymphoma 2016
    2016 Ascorbic acid intake and oxalate synthesis 2016
    2016 New World and Old World Alphaviruses Have Evolved to Exploit Different Components of Stress Granules, FXR and G3BP Proteins, for Assembly of Viral Replication Complexes 2016
    2016 Isolation, identification, and characterization of novel nanovesicles 2016
    2016 Hyperoxia Induces Intracellular Acidification in Neonatal Mouse Lung Fibroblasts: Real-Time Investigation Using Plasmonically Enhanced Raman Spectroscopy 2016
    2016 Surveying the serologic proteome in a tissue-specific kras(G12D) knockin mouse model of pancreatic cancer 2016
    2016 Fibronectin on the surface of myeloma cell-derived exosomes mediates exosome-cell interactions 2016
    2016 Conditioned media from adipose-derived stromal cells accelerates healing in 3-dimensional skin cultures 2016
    2016 LRRK2 autophosphorylation enhances its GTPase activity 2016
    2015 SSBP3 interacts with islet-1 and ldb1 to impact pancreatic β-cell target genes 2015
    2015 The G2019S LRRK2 mutation increases myeloid cell chemotactic responses and enhances LRRK2 binding to actin-regulatory proteins 2015
    2015 Differential proteomic and behavioral effects of long-term voluntary exercise in wild-type and APP-overexpressing transgenics 2015
    2015 Tyrosyl-DNA phosphodiesterase I catalytic mutants reveal an alternative nucleophile that can catalyze substrate cleavage 2015
    2015 12/15-Lipoxygenase-dependent ROS production is required for diet-induced endothelial barrier dysfunction 2015
    2015 Comparative proteomics analysis of high n-butanol producing metabolically engineered Clostridium tyrobutyricum 2015
    2014 Alterations in the rat serum proteome induced by prepubertal exposure to bisphenol A and genistein 2014
    2014 Vascular endothelial tight junctions and barrier function are disrupted by 15(S)-Hydroxyeicosatetraenoic acid partly via Protein Kinase C e-mediated Zona Occludens-1 Phosphorylation at Threonine 770/772 2014
    2014 Perk-dependent activation of JAK1 and STAT3 contributes to endoplasmic reticulum stress-induced inflammation 2014
    2014 The effects of frozen tissue storage conditions on the integrity of RNA and protein 2014
    2013 LRRK2 secretion in exosomes is regulated by 14-3-3 2013
    2013 Identification of gyrB and rpoB gene mutations and differentially expressed proteins between a novobiocin-resistant Aeromonas hydrophila catfish vaccine strain and its virulent parent strain 2013
    2013 Characterization of FKGK18 as Inhibitor of Group VIA Ca2+-Independent Phospholipase A2 (iPLA2β): Candidate Drug for Preventing Beta-Cell Apoptosis and Diabetes 2013
    2013 Parasitoid wasp venom SERCA regulates Drosophila calcium levels and inhibits cellular immunity 2013
    2013 Integrative Approach Reveals Composition of Endoparasitoid Wasp Venoms 2013
    2013 Exosome-like nanoparticles from intestinal mucosal cells carry prostaglandin E2 and suppress activation of liver NKT cells 2013
    2013 Identification, virulence, and mass spectrometry of toxic ECP fractions of West Alabama isolates of Aeromonas hydrophila obtained from a 2010 disease outbreak 2013
    2013 Grape exosome-like nanoparticles induce intestinal stem cells and protect mice from DSS-induced colitis 2013
    2012 Validation of a robust proteomic analysis carried out on formalin-fixed paraffin-embedded tissues of the pancreas obtained from mouse and human 2012
    2012 A cloud computing system to quickly implement new microarray data pre-processing methods 2012
    2012 Altered carcinogenesis and proteome in mammary glands of rats after prepubertal exposures to the hormonally active chemicals bisphenol A and genistein 2012
    2012 Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73 2012
    2012 Tumor cell cross talk with tumor-associated leukocytes leads to induction of tumor exosomal fibronectin and promotes tumor progression 2012
    2011 Autophosphorylation in the leucine-rich repeat kinase 2 (LRRK2) GTPase domain modifies kinase and GTP-binding activities 2011
    2011 Proteomic discovery of genistein action in the rat mammary gland 2011
    2011 Self-calibrated warping for mass spectra alignment 2011
    2010 Clustered O-glycans of IgA1: Defining macro- and microheterogeneity by use of electron capture/transfer dissociation 2010
    2010 Abundant lipid and protein components of drusen 2010
    2010 Proteomic analysis in mammary glands of rat offspring exposed in utero to bisphenol A 2010
    2010 Branched-chain amino acid metabolon: Interaction of glutamate dehydrogenase with the mitochondrial branched-chain aminotransferase (BCATm) 2010
    2009 Adipose tissue exosome-like vesicles mediate activation of macrophage-induced insulin resistance 2009
    2009 Detection of early unfolding events in a dimeric protein by amide proton exchange and native electrospray mass spectrometry 2009
    2009 Induction of myeloid-derived suppressor cells by tumor exosomes 2009
    2009 Structural heterogeneity and protein composition of exosome-like vesicles (prostasomes) in human semen 2009
    2008 Applying proteomic-based biomarker tools for the accurate diagnosis of pancreatic cancer 2008
    2008 Imaging mass spectrometry of intact proteins from alcohol-preserved tissue specimens: Bypassing formalin fixation 2008
    2008 Capsid Size Determination by Staphylococcus aureus Pathogenicity Island SaPI1 Involves Specific Incorporation of SaPI1 Proteins into Procapsids 2008
    2008 Differentiating Proteomic Biomarkers in Breast Cancer by Laser Capture Microdissection and MALDI MS 2008
    2008 Monitoring mouse prostate development by profiling and imaging mass spectrometry 2008
    2008 Incorporation of scaffolding protein gpO in bacteriophages P2 and P4 2008
    2007 A novel branched-chain amino acid metabolon: Protein-protein interactions in a supramolecular complex 2007
    2007 Processing MALDI mass spectra to improve mass spectral direct tissue analysis 2007
    2006 New developments in profiling and imaging of proteins from tissue sections by MALDI mass spectrometry 2006
    2006 A novel histology-directed strategy for MALDI-MS tissue profiling that improves throughput and cellular specificity in human breast cancer 2006
    2006 Selective profiling of proteins in lung cancer cells from fine-needle aspirates by matrix-assisted laser desorption ionization time-of-flight mass spectrometry 2006
    2005 Mass profiling-directed isolation and identification of a stage-specific serologic protein biomarker of advanced prostate cancer 2005
    2005 Overexpression of cytochrome P450 1A1 and its novel spliced variant in ovarian cancer cells: Alternative subcellular enzyme compartmentation may contribute to carcinogenesis 2005
    2004 The novel estrogen 17α-20Z-21-[(4-amino)phenyl]-19-norpregna-1,3,5(10),20-tetraene-3, 17β-diol induces apoptosis in prostate cancer cell lines at nanomolar concentrations in vitro 2004
    2004 A novel diagnostic test for prostate cancer emerges from the determination of α-methylacyl-coenzyme A racemase in prostatic secretions 2004
    2004 Estrogen receptor-mediated regulation of oxidative stress and DNA damage in breast cancer 2004
    2004 Monitoring the serological proteome: the latest modality in prostate cancer detection. 2004
    2003 Branched fatty acids in dairy and beef products markedly enhance α-methylacyl-CoA racemase expression in prostate cancer cells in vitro 2003
    2003 Synthesis and estrogen receptor binding affinities of 7-hydroxy-3-(4-hydroxyphenyl)-4H-1-benzopyran-4-ones containing a basic side chain 2003
    2002 Increasing the DNA damage threshold in breast cancer cells 2002
    2001 Effects of phytoestrogens and synthetic combinatorial libraries on aromatase, estrogen biosynthesis, and metabolism 2001
    1999 Measurement of oxidative DNA damage by catechol estrogens and analogues in vitro 1999

    Research Overview

  • Identification of disease-specific and clinically relevant markers of any disease/ disease process; application of high-level informatics to single and multiomics datasets for use in translational studies of disease
  • Investigator On

  • Molecular and Cellular Basis of Neurodevelopmental Disorders  awarded by National Institute of Mental Health/NIH/DHHS 2020 - 2025
  • Novel Therapeutics For Acquired Thrombotic Thrombocytopenic Purpura  awarded by National Heart, Lung, and Blood Institute/NIH/DHHS 2019 - 2023
  • Biosynthetic Metabolic Pathway Regulation of Glioma Growth  awarded by National Institute of Neurological Disorders and Stroke/NIH/DHHS 2017 - 2022
  • Racial Differences in the Natriuretic Peptide Response to Exercise and Beta-blockers  awarded by American Heart Association 2018 - 2021
  • Comprehensive Cancer Center Core Support Grant  awarded by National Cancer Institute/NIH/DHHS 2019 - 2021
  • Cell Type Specificity of NF1 Binding Partners and Effects of Variants with Divergent Genotype Phenotype Correlations  awarded by Johns Hopkins University 2020
  • Analysis Of Novel Molecular Mechanisms Regulating Expression Of The Adaptor Protein HSH2 And Class-Switched Antibody Production  awarded by National Institute of Allergy and Infectious Diseases/NIH/DHHS 2018 - 2020
  • Developmental and HyperActive Ras Tumor SPORE  awarded by Indiana University 2020
  • A Novel Role for IGF-1 Receptor in Growth Hormone Action  awarded by National Institute of Diabetes and Digestive and Kidney Diseases/NIH/DHHS 2015 - 2019
  • Alveolar DevMAP  awarded by National Heart, Lung, and Blood Institute/NIH/DHHS 2014 - 2019
  • Comprehensive Cancer Center Core Support Grant  awarded by National Cancer Institute/NIH/DHHS 2017 - 2019
  • UAB / UMN SPORE in Pancreatic Cancer  awarded by National Cancer Institute/NIH/DHHS 2010 - 2017
  • Comprehensive Cancer Center Core Support Grant  awarded by National Cancer Institute/NIH/DHHS 2016 - 2017
  • LRRK2 and Other Novel Exosome Proteins in Parkinson's Disease  awarded by National Institute of Neurological Disorders and Stroke/NIH/DHHS 2012 - 2016
  • Effects of 14-3-3s on Alpha-Synuclein Release and Toxicity  awarded by American Parkinson Disease Association 2014 - 2016
  • UAB / UMN SPORE in Pancreatic Cancer - Project 1  awarded by National Cancer Institute/NIH/DHHS 2010 - 2016
  • Combinational Environmental Chemicals Altering Susceptibility for Mammary Cancer  awarded by National Institute of Environmental Health Sciences/NIH/DHHS 2010 - 2016
  • Endogenous Oxalate Synthesis  awarded by National Institute of Diabetes and Digestive and Kidney Diseases/NIH/DHHS 2013 - 2016
  • Comprehensive Cancer Center Core Support Grant  awarded by National Cancer Institute/NIH/DHHS 2011 - 2016
  • Comprehensive Cancer Center Core Support Grant  awarded by National Cancer Institute/NIH/DHHS 2006 - 2012
  • Education And Training

  • Doctor of Pharmacy in Medicinal/Pharmaceutical Chemistry, Ohio State University System : Columbus 2000
  • Full Name

  • James Mobley