• I am a board-certified pathologist with >25-year experience in light-, immunofluorescence-, and high-resolution confocal microscopy. I worked as a staff pathologist at the UAB Hospital and HSF for 18 years. From October 2021, I am an Associate Professor in the Department of Microbiology. In my research projects, I have been involved in numerous collaborative studies and evaluated human, mouse, and rat tissues. Pathologic assessments have included organs and tissues from models of bacterial and viral infection, immune-complex deposition diseases, and histopathology of female reproductive organs ranging from precancerous lesions to invasive carcinoma. My role in these studies was to evaluate and grade histopathologic changes, perform immunofluorescence staining and do high-resolution imaging studies using laser scanning confocal microscopy. Image analysis involved morphometric and colocalization studies. My previous experience involved human islet cells isolation and pre-transplant preparation, pump perfusion of rat kidney and heart, quantification of extracellular matrix in kidney and heart, acute and chronic renal rejection, and HIV-virion identification in the intestinal tissue. Most recently, I evaluated a passive mouse model of IgA nephropathy using image analysis with morphometric, densitometric, and co-localization studies.

    I am offering following services through Histopathology Service Center supported by the Microbiology Department:
    • Histopathologic examination of human, mouse, and rat tissues
    (Bacterial and viral infection, immune-complex deposition diseases, precancerous lesions, invasive carcinoma)
    • Grading of histopathologic changes in hematoxylin-eosin-stained tissues
    • Evaluation of immunohistochemical staining
    • Image analysis (measurement of length and area, quantification)
    • High-resolution brightfield images of the tissue/lesions
    • The whole slide imaging (stitching)
    • Results provided in Excel sheet
    • Collaboration on writing the manuscript and poster presentation
  • Selected Publications

    Academic Article

    Year Title Altmetric
    2022 Nontypeable Haemophilus influenzae Infection Impedes Pseudomonas aeruginosa Colonization and Persistence in Mouse Respiratory TractInfection and Immunity.  90. 2022
    2022 Sequential modulation of the Wnt/β-catenin signaling pathway enhances tumor-intrinsic MHC I expression and tumor clearanceGynecologic Oncology.  164:170-180. 2022
    2021 Iga vasculitis with nephritis in adults: Histological and clinical assessmentJournal of Clinical Medicine.  10. 2021
    2021 Mesangioproliferative Kidney Diseases and Platelet-Derived Growth Factor–Mediated AXL PhosphorylationKidney Medicine.  3:1003-1013.e1. 2021
    2021 Experimental evidence of pathogenic role of IgG autoantibodies in IgA nephropathyJournal of Autoimmunity.  118. 2021
    2020 Large, Non-Cavity Distorting Intramural Leiomyomas Decrease Leukemia Inhibitory Factor in the Secretory Phase EndometriumReproductive Sciences.  27:569-574. 2020
    2020 The Reliability of Intraoperative Assessment on Predicting Tumor Size, Myometrial Invasion, and Cervical Involvement in Patients with a Preoperative Diagnosis of Complex Atypical Hyperplasia or (Clinical Stage I) Endometrial Cancer: A Prospective Cohort Study 2020
    2019 Glomerular immunodeposits of patients with IgA nephropathy are enriched for IgG autoantibodies specific for galactose-deficient IgA1Journal of the American Society of Nephrology.  30:2017-2026. 2019
    2018 Treatment of low-grade endometrial stromal sarcoma in a nulligravid womanGynecologic Oncology.  151:6-9. 2018
    2018 Severity and properties of cardiac damage caused by Streptococcus pneumoniae are strain dependentPLoS One.  13. 2018
    2018 Molecular response to neoadjuvant chemotherapy in high-grade serous ovarian carcinomaMolecular Cancer Research.  16:813-824. 2018
    2017 ACaseofaChorionicBump:New Sonographic-Histopathologic Findings With Review of the LiteratureJournal of Ultrasound in Medicine.  36:1968-1970. 2017
    2017 Malignant Brenner tumor associated with a germline BRCA2 mutationGynecologic Oncology Reports.  21:17-19. 2017
    2016 First-in-human trial of MIV-150 and zinc acetate coformulated in a carrageenan gel: Safety, pharmacokinetics, acceptability, adherence, and pharmacodynamics 2016
    2016 PcpA promotes higher levels of infection and modulates recruitment of myeloid-derived suppressor cells during pneumococcal pneumoniaJournal of Immunology.  196:2239-2248. 2016
    2015 The effects of CFTR and mucoid phenotype on susceptibility and innate immune responses in a mouse model of pneumococcal lung diseasePLoS One.  10. 2015
    2015 New Insights into the Pathogenesis of IgA Nephropathy.Kidney Diseases.  1:8-18. 2015
    2014 Cytomegalovirus enhances macrophage TLR expression and MyD88-mediated signal transduction to potentiate inducible inflammatory responsesJournal of Immunology.  193:5604-5612. 2014
    2014 Obesity is associated with higher 4E-BP1 expression in endometrial cancer.Current Biomarker Findings.  2014:1-7. 2014
    2014 Extramedullary hematopoiesis in uterine leiomyoma associated with numerous intravascular thrombi.Case Reports in Pathology.  2014:957395. 2014
    2014 Vaginal myeloid dendritic cells transmit founder HIV-1Journal of Virology.  88:7683-7688. 2014
    2013 IgA nephropathy: Molecular mechanisms of the diseaseAnnual Review of Pathology: Mechanisms of Disease.  8:217-240. 2013
    2011 IgA1 immune complexes from pediatric patients with IgA nephropathy activate cultured human mesangial cellsNephrology Dialysis Transplantation.  26:3451-3457. 2011
    2011 Mice overexpressing BAFF develop a commensal flora-dependent, IgA-associated nephropathyJournal of Clinical Investigation.  121:3991-4002. 2011
    2011 Stromal down-regulation of macrophage CD4/CCR5 expression and NF-κB activation mediates HIV-1 non-permissiveness in intestinal macrophagesPLoS Pathogens.  7. 2011
    2010 Juxtatumoral stromal reactions in uterine endometrioid adenocarcinoma and their prognostic significance 2010
    2010 Inflammation anergy in human intestinal macrophages is due to Smad-induced IκBα expression and NF-κB inactivationJournal of Biological Chemistry.  285:19593-19604. 2010
    2010 Human primary gastric dendritic cells induce a Th1 response to H. pyloriMucosal Immunology.  3:260-269. 2010
    2010 Dendritic cells transmit HIV-1 through human small intestinal mucosaJournal of Leukocyte Biology.  87:663-670. 2010
    2010 Impact of a weekly multidisciplinary tumor board conference on the management of women with gynecologic malignanciesInternational Journal of Gynecological Cancer.  20:103A. 2010
    2009 p16 improves interobserver agreement in diagnosis of anal intraepithelial neoplasiaJournal of Lower Genital Tract Disease.  13:145-153. 2009
    2009 Macrophages in vaginal but not intestinal mucosa are monocyte-like and permissive to human immunodeficiency virus type 1 infectionJournal of Virology.  83:3258-3267. 2009
    2008 Delivery of DNA HIV-1 vaccine to the liver induces high and long-lasting humoral immune responsesVaccine.  26:1541-1551. 2008
    2008 Atrial natriuretic peptide inhibits transforming growth factor β-induced Smad signaling and myofibroblast transformation in mouse cardiac fibroblastsCirculation Research.  102:185-192. 2008
    2008 IgA1-secreting cell lines from patients with IgA nephropathy produce aberrantly glycosylated IgA1Journal of Clinical Investigation.  118:629-639. 2008
    2008 Role of aberrant glycosylation of IgA1 molecules in the pathogenesis of iga nephropathyKidney and Blood Pressure Research.  31:29-37. 2008
    2008 Helicobacter pylori Gastritis in Children Is Associated With a Regulatory T-Cell ResponseGastroenterology.  134:491-499. 2008
    2007 Genistein attenuates the hypertensive effects of dietary NaCl in hypertensive male rats 2007
    2007 Estrogen modulates TNF-α-induced inflammatory responses in rat aortic smooth muscle cells through estrogen receptor-β activation 2007
    2007 ANP signaling inhibits TGF-β-induced Smad2 and Smad3 nuclear translocation and extracellular matrix expression in rat pulmonary arterial smooth muscle cellsJournal of Applied Physiology.  102:390-398. 2007
    2007 Human breast fibroblasts inhibit growth of the MCF10AT xenograft model of proliferative breast diseaseAmerican Journal of Pathology.  170:1064-1076. 2007
    2006 A prospective blinded evaluation of the accuracy of frozen section for the surgical management of endometrial cancerObstetrics and Gynecology.  108:1375-1379. 2006
    2006 Cytomegalovirus blocks intestinal stroma-induced down-regulation of macrophage HIV-1 infectionJournal of Leukocyte Biology.  80:1111-1117. 2006
    2006 Recurrence of endometrial adenocarcinoma in a prior Bartholin's cyst marsupialization incisionGynecologic Oncology.  103:749-751. 2006
    2006 Mucosal IL-8 and TGF-β recruit blood monocytes: Evidence for cross-talk between the lamina propria stroma and myeloid cellsJournal of Leukocyte Biology.  80:492-499. 2006
    2005 Nasal colonization with Streptococcus pneumoniae includes subpopulations of surface and invasive pneumococciInfection and Immunity.  73:6945-6951. 2005
    2005 IgA1-containing immune complexes in IgA nephropathy differentially affect proliferation of mesangial cellsKidney International.  67:504-513. 2005
    2004 AL-amyloidosis is underdiagnosed in renal biopsiesNephrology Dialysis Transplantation.  19:3050-3053. 2004
    2004 Estradiol and Progestins Differentially Modulate Leukocyte Infiltration after Vascular InjuryCirculation.  109:234-241. 2004
    2003 Immunizations with pneumococcal surface protein A and pneumolysin are protective against pneumonia in a murine model of pulmonary infection with Streptococcus pneumoniaeJournal of Infectious Diseases.  188:339-348. 2003
    2003 Mitochondrial targets of oxidative stress during renal ischemia/reperfusionMolecular cell biology research communications : MCBRC.  412:27-33. 2003
    2003 Multiple Langerhans cell nodules in an incidental thymectomyArchives of Pathology and Laboratory Medicine.  127:218-220. 2003
    2002 Interactions of human mesangial cells with IgA and IgA-containing immune complexesKidney International.  62:465-475. 2002
    1999 MR imaging of an extrauterine lipoleiomyomaAmerican Journal of Roentgenology.  172:1144-1145. 1999
    1997 Transposon mutagenesis: Cloning of chromosomal DNA from the site of Tn916 insertion using polymerase chain reaction 1997

    Research Overview

  • My research is based on projects which require histopathologic evaluation of the tissue and cells, light and confocal microscopy and image analysis.The most important manuscripts are concerning IgA nephropathy and gynecologic diseases
  • Education And Training

  • Baptist Medical Center - Montclair, Internship
  • Thomayer Hospital Gynecology, Residency
  • UAB Hospital Pathology, Residency
  • UAB Hospital Pathology, Postdoctoral Fellowship
  • Doctor of Medicine, Charles University in Prague 1985
  • Full Name

  • Lea Novakova