Positions

Selected Publications

Academic Article

Year Title Altmetric
2019 Out of the Curricular Shadows: Revolutionizing Undergraduate Immunology EducationFrontiers in Immunology.  10. 2019
2016 TREM-like transcript 2 is stored in human neutrophil primary granules and is up-regulated in response to inflammatory mediatorsJournal of Leukocyte Biology.  100:177-184. 2016
2016 Biomedical science postdocs: An end to the era of expansionFederation proceedings.  30:41-44. 2016
2016 Family history of hematologic malignancies and risk of multiple myeloma: differences by race and clinical featuresCancer Causes and Control.  27:81-91. 2016
2014 Putting PhDs to work: Career planning for today's scientistCBE : Life Sciences Education.  13:49-53. 2014
2014 Murine marginal zone B cells play a role in Vibrio cholerae LPS antibody responsesPathogens and Disease.  70:153-157. 2014
2013 The Actin and Tetraspanin Networks Organize Receptor Nanoclusters to Regulate B Cell Receptor-Mediated SignalingImmunity.  38:461-474. 2013
2013 Lymphotoxin α1β2 expression on B cells is required for follicular dendritic cell activation during the germinal center responseEuropean Journal of Immunology.  43:348-359. 2013
2012 Erratum: Writing a first grant proposal (Nature Immunology (2011) 13 (105-108))Nature Immunology.  13:621. 2012
2012 Writing a first grant proposalNature Immunology.  13:106-108. 2012
2011 Differential expression of the adaptor protein HSH2 controls the quantitative and qualitative nature of the humoral responseJournal of Immunology.  187:3565-3577. 2011
2011 TLT2 potentiates neutrophil antibacterial activity and chemotaxis in response to G protein-coupled receptor-mediated signalingJournal of Immunology.  187:2346-2355. 2011
2011 Marginal zone B cells regulate antigen capture by marginal zone macrophagesJournal of Immunology.  186:2172-2181. 2011
2006 Trem-like transcript 2 is expressed on cells of the myeloid/granuloid and B lymphoid lineage and is up-regulated in response to inflammationJournal of Immunology.  176:6012-6021. 2006
2006 Expression of the adaptor protein hematopoietic Src homology 2 is up-regulated in response to stimuli that promote survival and differentiation of B cellsJournal of Immunology.  176:4163-4172. 2006
2005 The adaptor protein HSH2 attenuates apoptosis in response to ligation of the B cell antigen receptor complex on the B lymphoma cell line, WEHI-231Journal of Biological Chemistry.  280:3507-3515. 2005
2003 The B cell coreceptor CD22 associates with AP50, a clathrin-coated pit adapter protein, via tyrosine-dependent interactionJournal of Immunology.  170:3534-3543. 2003
2002 Evaluating function of transmembrane protein tyrosine phosphatase CD148 in lymphocyte biologyImmunologic Research.  26:153-166. 2002
2002 AP2 adaptor complex-dependent internalization of CD5: Differential regulation in T and B cellsJournal of Immunology.  168:5612-5620. 2002
2001 CD45 function is regulated by an acidic 19-amino acid insert in domain II that serves as a binding and phosphoacceptor site for casein kinase 2Journal of Immunology.  166:7208-7218. 2001
2001 The role of the protein tyrosine phosphatase CD45 in regulation of B lymphocyte activationInternational Reviews of Immunology.  20:713-738. 2001
2000 Regulation of MHC class II signal transduction by the B cell coreceptors CD19 and CD22Journal of Immunology.  165:5588-5596. 2000
2000 Signal transduction and the coordination of B lymphocyte development and function I. Transduction of BCR signals from the cell membrane to the nucleus. IntroductionCurrent Topics in Microbiology and Immunology.  245:V-X. 2000
1999 Signal transduction via the B-cell antigen receptor: The role of protein tyrosine kinases and protein tyrosine phosphatasesCurrent Topics in Microbiology and Immunology.  245 I:1-51. 1999
1999 Analysis of tyro sine phosphorylation-dependent interactions between stimulatory effector proteins and the B cell co-receptor CD22Journal of Biological Chemistry.  274:18769-18776. 1999
1999 CD45 regulates tyrosine phosphorylation of CD22 and its association with the protein tyrosine phosphatase SHP-1Journal of Immunology.  162:5278-5286. 1999
1998 Major histocompatibility class II-mediated signal transduction is regulated by the protein-tyrosine phosphatase CD45Journal of Biological Chemistry.  273:11970-11979. 1998
1998 Association of MHC class II molecules with transmembrane protein(s) is required for the formation of a competent signaling complex in B cellsFederation proceedings.  12. 1998
1998 CD45 regulates the interaction between CD22 and SHP-1 in B cellsFederation proceedings.  12. 1998
1998 Interaction of AP50 with the cytoplasmic domain of murine CD22.2Federation proceedings.  12. 1998
1997 Kinase-Independent Potentiation of B Cell Antigen Receptor-Mediated Signal Transduction by the Protein Tyrosine Kinase SrcJournal of Immunology.  159:4823-4833. 1997
1997 Recruitment and phosphorylation of SH2-containing inositol phosphatase and Shc to the B-cell Fcγ immunoreceptor tyrosine-based inhibition motif peptide motifMolecular and Cellular Biology.  17:4305-4311. 1997
1997 The role of CD45 in signal transductionAdvances in Immunology.  66:1-65. 1997
1996 Yersinia enterocolitica envelope proteins that are crossreactive with the thyrotropin receptor (TSHR) also have B-cell mitogenic activityJournal of Autoimmunity.  9:509-516. 1996
1995 Mechanism of inhibition of eosinophil activation by transforming growth factor-β - Inhibition of Lyn, MAP, Jak2 kinases and STAT1 nuclear factorJournal of Immunology.  155:4454-4458. 1995
1995 The intracellular signal transduction mechanism of interleukin 5 in eosinophils: The involvement of lyn tyrosine kinase and the ras-raf-1-MEK-microtubule-associated protein kinase pathwayJournal of Experimental Medicine.  181:1827-1834. 1995
1995 A role in B cell activation for CD22 and the protein tyrosine phosphatase SHPScience.  269:242-244. 1995
1995 Potentiation of B‐Cell Antigen Receptor‐mediated Signal Transduction by the Heterologous src Family Protein Tyrosine Kinase, srcAnnals of the New York Academy of Sciences.  766:214-215. 1995
1994 Multiple components of the B cell antigen receptor complex associate with the protein tyrosine phosphatase, CD45Journal of Biological Chemistry.  269:17238-17244. 1994
1994 Regulation of B-cell activation by CD45: a question of mechanismImmunology Today.  15:399-406. 1994
1993 Administration of anti-CD45 mAb specific for a B cell-restricted epitope abrogates the B cell response to a T-dependent antigen in vivoJournal of Immunology.  151:5936. 1993
1992 Regulation of basal tyrosine phosphorylation of the B cell antigen receptor complex by the protein tyrosine phosphatase, CD45Journal of Immunology.  149:3182-3190. 1992
1992 The MB-1/B29 heterodimer couples the B cell antigen receptor to multiple src family protein tyrosine kinasesJournal of Immunology.  149:1548-1555. 1992
1991 Regulation of B cell antigen receptor signal transduction and phosphorylation by CD45Science.  252:1839-1842. 1991
1991 Regulation of B cell antigen receptor signal transduction and phosphorylation by CD45.Science.  252. 1991
1991 The B-cell antigen receptor complexImmunology Today.  12:196-201. 1991
1990 Endogenously secreted IL-4 is required for mouse thymocytes to become cytotoxic. Human, but not mouse, IL-2 induces a functionally immature thymic subset to secrete IL-4 and become CTLImmunology.  70:478-484. 1990
1990 Improved method for measuring intracellular Ca++ with fluo‐3Cytometry.  11:923-927. 1990
1990 Membrane IgM and IgD molecules fail to transduce Ca2+ mobilizing signals when expressed on differentiated B lineage cellsJournal of Immunology.  144:3272-3280. 1990
1989 Induction of c-fos and c-myc expression during B cell activation by IL-4 and immunoglobulin binding ligandsJournal of Immunology.  143:1032-1039. 1989
1989 Production of multiple lymphokines by the A20.1 B cell lymphoma after cross-linking of membrane Ig by immobilized anti-IgJournal of Immunology.  143:881-889. 1989
1988 B cell stimulatory factor 1 induces lobster agglutinin 1-separated mouse thymocytes to express cytotoxic T lymphocyte activityJournal of Immunology.  141:145-150. 1988
1988 Biochemical characterization of proteins that co-purify with class II antigens of the murine MHCJournal of Immunology.  140:1930-1938. 1988
1987 Coupling of B cell surface Ig, Ia and BSF1 receptors to intracellular "second messengers".Advances in Experimental Medicine and Biology.  213:195-205. 1987
1987 Ia binding ligands and cAMP stimulate nuclear translocation of PKC in B lymphocytesNature.  327:629-632. 1987
1987 Transmembrane Signals and Intracellular “Second Messengers” in the Regulation of Quiescent B‐Lymphocyte ActivationImmunological Reviews.  95:37-57. 1987
1986 BSF1 induces membrane protein phosphorylation but not phosphoinositide metabolism, Ca2+ mobilization, protein kinase C translocation, or membrane depolarization in resting murine B lymphocytesJournal of Immunology.  137:3664-3670. 1986
1986 Modulation of cyclic AMP-dependent protein kinase isozyme expression associated with activation of a macrophage cell lineJournal of Immunology.  136:270-277. 1986
1984 Differential expression of cAMP dependent protein kinases in operationally defined populations of macrophagesFederation proceedings.  43. 1984
1984 Inhibition of macrophage-mediated tumor cell destruction by oxidized lipoproteinsJNCI: Journal of the National Cancer Institute.  73:469-474. 1984

Book

Year Title Altmetric
1999 Signal transduction and the coordination of B lymphocyte development and function II. Translation of BCR signals to specific physiologic outcomes: Preface.  Ed. 245 II.  1999
1999 Signal transduction and the coordination of B lymphocyte development and function I: Preface.  Ed. 245 I.  1999

Chapter

Year Title Altmetric
2010 Signal transduction via the b cell antigen receptor. a crucial regulator of b cell biology.  2689-2698. 2010
2003 Signal Transduction via the B-Cell Antigen Receptor: A Crucial Regulator of B-Cell Biology.  555-564. 2003

Research Overview

  • Analysis of the Molecular and Functional Role of the Adaptor Protein HSH2. Studies are ongoing to elucidate the functional role that the adaptor protein HSH2 plays in regulating B cell biology. HSH2 is selectively expressed in cells of the B lineage and its expression is up-regulated in vitro in response to agonists that promote B cell survival and differentiation, including CD40L, BLyS, LPS and CpG DNA. Recent studies using transgenic mice have revealed that HSH2 plays a critical role in regulating terminal differentiation of B cells into antibody secreting plasma cells. Constitutive expression of HSH2 in the B lineage results in a significant decrease in serum immunoglobulin (Ig) titers. Moreover, immunization of HSH2 Tg mice with either T-dependent or T-independent antigens induces only a modest antigen-specific IgM response and very little class-switched Ig production. Conversely immunization of mice in which HSH2 is expressed at only one fifth the normal amount in B cells (HSH2 hypomorph mice), leads to enhanced production of antigen-specific class switched Ig both in terms of the kinetics and magnitude of the response when compared to wild type mice. Based on these observations, it is likely that HSH2 plays a critical role in regulating Ig class switching and terminal differentiation of B cells. Future studies will be conducted to:

    Identify important regions/motifs of HSH2 that are involved in its function
    Identify the proteins that interact with HSH2 in B lymphocytes and assess their functional importance
    Characterize the molecular mechanisms that control HSH2 expression in B cells
    Generate conditional knockout mice to examine the importance of HSH2 in regulation of B cell development, activation and differentiation
    Analysis of the Molecular and Functional Role of the Transmembrane Receptor Trem-Like Transcript 2 (TLT2). The genes encoding mouse and human TLT2 were cloned in our laboratory. Subsequent experiments in mice demonstrated that TLT2 is expressed on B cells, neutrophils and macrophages. With respect to the B lineage, TLT2 is expressed early during development, prior to the BCR and can be detected on both fetal liver- and bone marrow-derived B cell progenitors. Although TLT2 is expressed on all B cells in the periphery, its level is higher on transitional, marginal zone and B-1 B cells when compared to follicular B cells. Expression of TLT2 can be detected on peritoneal and alveolar macrophages but not on monocytes in the blood. Finally, TLT2 is expressed on neutrophils and neutrophil progenitors. Importantly, TLT2 is significantly up-regulated on macrophages and neutrophils in response to inflammatory stimuli such as LPS or Staphylococcal superantigens. Neutrophils also contain large pools or preformed TLT2 in their granules that are rapidly released in response to activating stimuli such as FMLP or PMA. Recent studies have shown that TLT2 plays an important role as a potentiating receptor that enhances the neutrophil response to agonists that bind to and signal through 7-transmembrane, G protein-coupled receptors. Specifically, TLT2 cross-linking enhances ROS production, degranulation and migration of neutrophils stimulated with several agonists, including fMLP, and various chemokines. Thus, TLT2 is likely to play an important role in the innate immune response and is likely to functionally bridge components of the innate response with the adaptive response. Future studies will be conducted to:

    Characterize TLT2 expression and function in human immune cells
    Assess the functional role played by TLT2 in immune responses to infectious organisms and during the inflammatory response; conditional knockout mice will be generated for these studies, as well as Tg mice that conditionally express a soluble form of TLT2.
    Identify the ligand(s) for TLT2 using several molecular and biochemical approaches
    Identify interacting signal transduction proteins and their associated pathways that mediate the functional effects of TLT2 on immune cells
    Characterization of Molecular Mechanisms that Regulate 1) Marginal Zone Development and Homeostasis and 2) Initiation and Maintenance of the Germinal Center Reaction. CD19 is a B cell transmembrane protein that is required for both the marginal zone (MZ) and germinal center (GC) B cell responses. The MZ provides a rapid response against pathogens that have entered the circulation, which otherwise can be deadly in hours. We have found that MZ macrophages are absent and dendritic cells were abnormally distributed in mice that lack CD19. Despite these structural defects, adoptive transfer studies demonstrate that failure of CD19-/- B cells to enter the MZ is B cell-intrinsic. Reconstitution of MZ B cells was followed by recovery of MZ macrophages and dendritic cells, suggesting that MZ B cells control the organization of other cells in the MZ. Studies will be conducted to:

    Determine the mechanisms by which CD19 controls differentiation and localization of MZ B cells
    Determine the mechanisms by which MZ B cells regulate other MZ cells
    The GC is the site of affinity maturation and the generation of B cell memory, which are essential for inducing long-lasting immunity with vaccines. Preliminary data indicate that Follicular Dendritic Cell (FDC) activation fails in mice that lack CD19. The lack of FDC activation is likely critical to the failure of the GC pathway, and hence T-dependent antibody responses. CD19 could contribute to FDC activation through effects on activation of follicular B cells, on formation of immune complexes through effects on IgM, and on MZ-dependent trafficking of antigen into the follicle. Studies will be performed to:

    Determine the role of CD19 in promoting LTb expression of GC B cells and the role of LTb in activation of FDC
    Determine the role of immune complex formation in the initiation of the GC response
    Examine the role of the VLA-4:VCAM-1 interaction between B cells and FDCs, respectively for maintenance of the GC response

    Personal Interests
    When not working he enjoys cooking and gardening, as well as fishing or hiking.
  • Teaching Activities

  • GBSC742 - Student Theme Meeting Course (Fall Term 2019) 2019
  • HC117 - Honors Seminar: Science/Math (Fall Term 2019) 2019
  • MIC250 - Seminars in Immunology (Fall Term 2019) 2019
  • MIC410 - Special Topics in Immunology (Fall Term 2019) 2019
  • MIC498 - Honors Research in Immunology (Fall Term 2019) 2019
  • STP2043 - SURVIVAL SKILLS FOR PHYSICIAN SCIENTISTS (Fall Term 2019) 2019
  • MIC410 - Special Topics in Immunology (Summer Term 2019) 2019
  • MIC498 - Honors Research in Immunology (Summer Term 2019) 2019
  • GBS740A - Intro to Immunology Part 1 (Spring Term 2019) 2019
  • GBS740B - Intro to Immunology Part 2 (Spring Term 2019) 2019
  • GBSC742 - Student Theme Meeting Course (Spring Term 2019) 2019
  • MIC150 - Current Topics In Immunology (Spring Term 2019) 2019
  • MIC275 - Introductory Immunology (Spring Term 2019) 2019
  • MIC498 - Honors Research in Immunology (Spring Term 2019) 2019
  • GBSC742 - Student Theme Meeting Course (Fall Term 2018) 2018
  • MIC250 - Seminars in Immunology (Fall Term 2018) 2018
  • STP2043 - SURVIVAL SKILLS FOR PHYSICIAN SCIENTISTS (Fall Term 2018) 2018
  • MSTP794 - Translational Research Seminar (Summer Term 2018) 2018
  • GBS740A - Intro to Immunology Part 1 (Spring Term 2018) 2018
  • GBS740B - Intro to Immunology Part 2 (Spring Term 2018) 2018
  • GBSC722 - Special Topics (Spring Term 2018) 2018
  • MIC450 - Current Topics in Immunology (Spring Term 2018) 2018
  • MSTP794 - Translational Research Seminar (Spring Term 2018) 2018
  • GBS740A - INTRODUCTION TO IMMUNOLOGY A (Spring Term 2018) 2018
  • GBSC722 - Special Topics (Fall Term 2017) 2017
  • MSTP794 - Translational Research Seminar (Fall Term 2017) 2017
  • STP2043 - SURVIVAL SKILLS FOR PHYSICIAN SCIENTISTS (Fall Term 2017) 2017
  • MSTP794 - Translational Research Seminar (Summer Term 2017) 2017
  • GBS740A - Intro to Immunology Part 1 (Spring Term 2017) 2017
  • GBS740B - Intro to Immunology Part 2 (Spring Term 2017) 2017
  • GBSC701 - Seminars (Spring Term 2017) 2017
  • MSTP794 - Translational Research Seminar (Spring Term 2017) 2017
  • GBS740B - INTRODUCTION TO IMMUNOLOGY B (Spring Term 2017) 2017
  • GBS740A - INTRODUCTION TO IMMUNOLOGY A (Spring Term 2017) 2017
  • MIC710 - Dev Comm Skills for Bio (Fall Term 2016) 2016
  • MIC799 - Dissert Res Molecular Cell Bio (Fall Term 2016) 2016
  • MSTP794 - Translational Research Seminar (Fall Term 2016) 2016
  • STP2043 - SURVIVAL SKILLS FOR PHYSICIAN SCIENTISTS (Fall Term 2016) 2016
  • MSTP794 - Translational Research Seminar (Summer Term 2016) 2016
  • GBS740A - Intro to Immunology Part 1 (Spring Term 2016) 2016
  • GBS740B - Intro to Immunology Part 2 (Spring Term 2016) 2016
  • MIC710 - Dev Comm Skills for Bio (Spring Term 2016) 2016
  • MSTP794 - Translational Research Seminar (Spring Term 2016) 2016
  • STP2043 - SURVIVAL SKILLS FOR PHYSICIAN SCIENTISTS (Fall Term 2015) 2015
  • MSTP794 - Translational Research Seminar (Summer Term 2015) 2015
  • 32-241 - MICROBIOLOGY RESEARCH (Fall Term 2015) 2015
  • MSTP794 - Translational Research Seminar (Spring Term 2015) 2015
  • STP2043 - SURVIVAL SKILLS FOR PHYSICIAN SCIENTISTS (Fall Term 2014) 2014
  • STP2043 - SURVIVAL SKILLS FOR PHYSICIAN SCIENTISTS (Fall Term 2013) 2013
  • STP2043 - SURVIVAL SKILLS FOR PHYSICIAN SCIENTISTS (Fall Term 2013) 2013
  • STP2043 - SURVIVAL SKILLS FOR PHYSICIAN SCIENTISTS (Fall Term 2013) 2013
  • 32-241 - MICROBIOLOGY RESEARCH (Spring Term 2013) 2013
  • STP2043 - SURVIVAL SKILLS FOR PHYSICIAN SCIENTISTS (Fall Term 2012) 2012
  • OBHS111 - Fundamentals of Dent and Opt I (Fall Term 2011) 2011
  • STP2043 - SURVIVAL SKILLS FOR PHYSICIAN SCIENTISTS (Fall Term 2011) 2011
  • STP2043 - SURVIVAL SKILLS FOR PHYSICIAN SCIENTISTS (Fall Term 2011) 2011
  • OBHS111 - Fundamentals of Dent and Opt I (Fall Term 2010) 2010
  • STP2043 - SURVIVAL SKILLS FOR PHYSICIAN SCIENTISTS (Fall Term 2010) 2010
  • MIC700 - Adv Course in Autoimmunity (Spring Term 2010) 2010
  • OBHS111 - Fundamentals of Dent and Opt I (Fall Term 2009) 2009
  • STP2043 - SURVIVAL SKILLS FOR PHYSICIAN SCIENTIST (Fall Term 2009) 2009
  • STP2043 - SURVIVAL SKILLS FOR PHYSICIAN SCIENTIST (Fall Term 2008) 2008
  • 01-530R - MICROBIOLOGY REMEDIATION (Fall Term 2008) 2008
  • 01-530 - MICROBIOLOGY/IMMUNOLOGY (Fall Term 2007) 2007
  • 01-530 - MICROBIOLOGY/IMMUNOLOGY (Fall Term 2007) 2007
  • Education And Training

  • Doctor of Philosophy in Microbiology, Ohio State University System : Columbus 1985
  • Full Name

  • Louis Justement
  • Fax

  • 205-996-6749