Positions

Selected Publications

Academic Article

Year Title Altmetric
2021 Duration of post-COVID-19 symptoms are associated with sustained SARS-CoV-2 specific immune responses.JCI insight2021
2021 Epitope Classification and RBD Binding Properties of Neutralizing Antibodies Against SARS-CoV-2 Variants of ConcernFrontiers in Immunology.  12. 2021
2021 Generation and characterization of recombinant SARS-CoV-2 expressing reporter genesJournal of Virology.  95. 2021
2021 Selection, identification, and characterization of SARS-CoV-2 monoclonal antibody resistant mutantsJournal of Virological Methods.  290. 2021
2021 Therapeutic activity of an inhaled potent SARS-CoV-2 neutralizing human monoclonal antibody in hamsters 2021
2021 Polyfunctional tier 2-neutralizing antibodies cloned following hiv-1 env macaque immunization mirror native antibodies in a human donorJournal of Immunology.  206:999-1012. 2021
2021 Sustained cellular immune dysregulation in individuals recovering from SARS-CoV-2 infectionJournal of Clinical Investigation.  131. 2021
2021 Rapid in vitro assays for screening neutralizing antibodies and antivirals against SARS-CoV-2Journal of Virological Methods.  287. 2021
2020 Impact of vaccine type on HIV-1 vaccine elicited antibody durability and B cell gene signatureScientific Reports.  10. 2020
2020 Cloning and functional testing of rhesus macaque (Macaca mulatta) IL-9 and IL-33Journal of Medical Primatology.  49:144-152. 2020
2020 Persistence of HIV-1 Env-Specific Plasmablast Lineages in Plasma Cells after Vaccination in Humans 2020
2020 A broad and potent H1-specific human monoclonal antibody produced in plants prevents influenza virus infection and transmission in Guinea pigsViruses.  12. 2020
2019 DNA priming and gp120 boosting induces HIV-specific antibodies in a randomized clinical trialJournal of Clinical Investigation.  129:4769-4785. 2019
2019 Identification of variant HIV envelope proteins with enhanced affinities for precursors to anti-gp41 broadly neutralizing antibodiesPLoS ONE.  14. 2019
2019 IL-33 enhances the kinetics and quality of the antibody response to a DNA and protein-based HIV-1 Env vaccineVaccine.  37:2322-2330. 2019
2019 Broad and protective influenza B virus neuraminidase antibodies in humans after vaccination and their clonal persistence as plasma cellsmBio.  10:1-17. 2019
2018 A highly potent and broadly neutralizing H1 influenza-specific human monoclonal antibodyScientific Reports.  8. 2018
2018 Display of the HIV envelope protein at the yeast cell surface for immunogen developmentPLoS ONE.  13. 2018
2017 Curaxin CBL0100 blocks HIV-1 replication and reactivation through inhibition of viral transcriptional elongationFrontiers in Microbiology.  8. 2017
2017 A novel bromodomain inhibitor reverses HIV-1 latency through specific binding with BRD4 to promote Tat and P-TEFb associationFrontiers in Microbiology.  8. 2017
2017 Pre-existing neutralizing antibody mitigates B cell dysregulation and enhances the Envspecific antibody response in SHIV-infected rhesus macaquesPLoS ONE.  12. 2017
2016 Increased Steady-State Memory B Cell Subsets among High-Risk Participants in an HIV Vaccine TrialAIDS Research and Human Retroviruses.  32:1143-1148. 2016
2016 Humoral dysregulation associated with increased systemic inflammation among injection heroin usersPLoS ONE.  11. 2016
2016 Identification of significant B cell associations with undetected observations using a Tobit modelStatistics and Its Interface.  9:79-91. 2016
2015 Platelet activation in human immunodeficiency virus type-1 patients is not altered with cocaine abusePLoS ONE.  10. 2015
2015 Functional and Molecular Characteristics of Novel and Conserved Cross-Clade HIV Envelope Specific Human Monoclonal AntibodiesMonoclonal Antibodies in Immunodiagnosis and Immunotherapy.  34:65-72. 2015
2014 Microbubble array diffusion assay for the detection of cell secreted factors 2014
2014 Cutting edge: An antibody recognizing ancestral endogenous virus glycoproteins mediates antibody-dependent cellular cytotoxicity on HIV-1-infected cellsJournal of Immunology.  193:1544-1548. 2014
2014 Transient decrease in human peripheral blood myeloid dendritic cells following influenza vaccination correlates with induction of serum antibody 2014
2013 Neutralizing polyclonal IgG present during acute infection prevents rapid disease onset in simian-human immunodeficiency virus SHIVSF162P3-infected infant rhesus macaquesJournal of Virology.  87:10447-10459. 2013
2013 Characterization of cell seeding and specific capture of B cells in microbubble well arraysBiomedical Microdevices.  15:453-463. 2013
2013 Anti-idiotypic monobodies derived from a fibronectin scaffoldBiochemistry.  52:1802-1813. 2013
2013 9G4+ antibodies isolated from HIV-infected patients neutralize HIV-1 and have distinct autoreactivity profilesPLoS ONE.  8. 2013
2012 Anti-idiotypic monobodies for immune response profilingMethods.  58:62-68. 2012
2012 9G4 autoreactivity is increased in HIV-infected patients and correlates with HIV broadly neutralizing serum activityPLoS ONE.  7. 2012
2011 Decreased influenza-specific B cell responses in rheumatoid arthritis patients treated with anti-tumor necrosis factor 2011
2010 Peak frequencies of circulating human influenza-specific antibody secreting cells correlate with serum antibody response after immunizationVaccine.  28:3582-3587. 2010
2010 Human basophils express amphiregulin in response to T cell-derived IL-3Journal of Allergy and Clinical Immunology.  126. 2010
2009 T helper cytokine patterns: Defined subsets, random expression, and external modulationImmunologic Research.  45:173-184. 2009
2009 Human follicular lymphoma CD39+-infiltrating T cells contribute to adenosine-mediated T cell hyporesponsivenessJournal of Immunology.  183:6157-6166. 2009
2009 An 11-color flow cytometric assay for identifying, phenotyping, and assessing endocytic ability of peripheral blood dendritic cell subsets in a single platformJournal of Immunological Methods.  341:106-116. 2009
2009 Frequencies of human influenza-specific antibody secreting cells or plasmablasts post vaccination from fresh and frozen peripheral blood mononuclear cellsJournal of Immunological Methods.  340:42-47. 2009
2008 Automated analysis of two- and three-color fluorescent Elispot (Fluorospot) assays for cytokine secretionComputer Methods and Programs in Biomedicine.  92:54-65. 2008
2007 Temporal and spatial changes of histone 3 K4 dimethylation at the IFN-γ gene during Th1 and Th2 cell differentiationJournal of Immunology.  179:6410-6415. 2007
2006 Amphiregulin, a TH2 cytokine enhancing resistance to nematodesScience.  314:1746. 2006
2006 T regulatory and primed uncommitted CD4 T cells express CD73, which suppresses effector CD4 T cells by converting 5′-adenosine monophosphate to adenosineJournal of Immunology.  177:6780-6786. 2006
2006 Short-term dietary administration of celecoxib enhances the efficacy of tumor lysate-pulsed dendritic cell vaccines in treating murine breast cancerInternational Journal of Cancer.  118:2220-2231. 2006
2005 CD73 and Ly-6A/E distinguish in vivo primed but uncommitted mouse CD4 T cells from type 1 or type 2 effector cellsJournal of Immunology.  175:6458-6464. 2005
2004 α-tocopheryl succinate sensitizes established tumors to vaccination with nonmatured dendritic cells 2004
2003 Immunosuppressive role of transforming growth factor beta in breast cancerClinical and Applied Immunology Reviews.  3:277-287. 2003
2003 Transforming growth factor β inhibits the antigen-presenting functions and antitumor activity of dendritic cell vaccinesCancer Research.  63:1860-1864. 2003
2001 Comparative analysis of IFN-γ B7.1 and antisense TGF-β gene transfer on the tumorigenicity of a poorly immunogenic metastatic mammary carcinoma 2001
2001 Invasion and metastasis of a mammary tumor involves TGF-β signalingInternational Journal of Cancer.  91:76-82. 2001
1994 Toughness response of electrodes within arc furnace cycling 1994
A broad and potent H1-specific human monoclonal antibody produced in plants prevents influenza virus infection and transmission in guinea pigs

Research Overview

  • The Kobie Laboratory is focused on translational B cell immunology. Through the development of high throughput and high resolution technologies the lab dissects the mechanisms of protective and pathogenic B cell and antibody responses to advance vaccine development and disease therapeutics, with a primary focus on HIV and influenza.
  • Education And Training

  • Doctor of Philosophy in Microbiology and Immunology, University of Arizona 2003
  • Full Name

  • James Kobie