Selected Publications

Academic Article

Year Title Altmetric
2021 Molecular mechanisms of inhibiting glucosyltransferases for biofilm formation in Streptococcus mutansInternational Journal of Oral Science.  13. 2021
2021 The ZO-1 protein Polychaetoid as an upstream regulator of the Hippo pathway in DrosophilaPLoS Genetics.  17. 2021
2021 Post-translational modification of Streptococcus sanguinis SpxB influences protein solubility and H2O2 productionMolecular Oral Microbiology.  36:267-277. 2021
2021 Structure-function characterization of Streptococcus intermedius surface antigen pasJournal of Bacteriology.  203. 2021
2021 Structural and functional analysis of the C-terminal region of Streptococcus gordonii SspBActa crystallographica. Section D, Structural biology.  77:1206-1215. 2021
2021 Insights Into the Oral Microbiome and Barrett's Esophagus Early Detection: A Narrative ReviewClinical and Translational Gastroenterology.  12:e00390. 2021
2021 Autism-Associated Variant in the SLC6A3 Gene Alters the Oral Microbiome and Metabolism in a Murine ModelFrontiers in Psychiatry.  12. 2021
2021 Multiple factors are involved in regulation of extracellular membrane vesicle biogenesis in Streptococcus mutansMolecular Oral Microbiology.  36:12-24. 2021
2021 Discovery of Potent Inhibitors of Streptococcus mutans Biofilm with Antivirulence ActivityACS Medicinal Chemistry Letters.  12:48-55. 2021
2021 Transcriptional programming in arteriosclerotic,disease; A Multifaceted Function of the Runx2 (Runt-Related Transcription Factor 2) 2021
2020 Caries-Associated Biosynthetic Gene Clusters in Streptococcus mutans 2020
2020 Intracellular metabolism analysis of Clostridium cellulovorans via modeling integrating proteomics, metabolomics and fermentationProcess Biochemistry.  89:9-19. 2020
2020 Pulp–dentin tissue healing response: A discussion of current biomedical approachesJournal of Clinical Medicine.  9. 2020
2020 Arterial Stiffness: A Focus on Vascular Calcification and Its Link to Bone Mineralization 2020
2019 Metabolic stress and cardiovascular disease in diabetes mellitus: The role of protein O -GlcNAc modification 2019
2019 Dietary Nitrite Drives Disease Outcomes in Oral Polymicrobial Infections 2019
2019 Signal transduction of streptococci by cyclic dinucleotide second messengersCurrent Issues in Molecular Biology.  32:87-122. 2019
2019 Cloning, expression, purification, and preliminary characterization of single-crystal X-ray diffraction of glucosyltransferase B of streptococcus mutans 2019
2019 Quantitative proteomics uncovers the interaction between a virulence factor and mutanobactin synthetases in Streptococcus mutansClinical and Vaccine Immunology.  4. 2019
2018 Antigen I/II mediates interactions between Streptococcus mutans and Candida albicansMolecular Oral Microbiology.  33:283-291. 2018
2018 Inhibition of Streptococcus mutans Biofilms by the Natural Stilbene Piceatannol Through the Inhibition of GlucosyltransferasesACS Omega.  3:8378-8385. 2018
2018 Correction: "Activation of the innate immune system by Treponema denticola periplasmic flagella through Toll-like receptor 2" [Infection and Immunity, 86, 1, e00573-17, (2018)] doi: 10.1128/IAI.00573-17Infection and Immunity.  86. 2018
2018 Glucan binding protein C of Streptococcus mutans mediates both sucrose-independent and sucrose-dependent adherenceInfection and Immunity.  86. 2018
2018 AKT-independent activation of p38 MAP kinase promotes vascular calcificationRedox Biology.  16:97-103. 2018
2018 Oral Biofilms: Pathogens, Matrix, and Polymicrobial Interactions in MicroenvironmentsTrends in Microbiology.  26:229-242. 2018
2018 Activation of the innate immune system by Treponema denticola periplasmic flagella through Toll-like receptor 2Infection and Immunity.  86. 2018
2018 Glycosyltransferase-mediated biofilm matrix dynamics and virulence of Streptococcus mutansApplied and Environmental Microbiology.  85. 2018
2018 Small molecule inhibitors for streptococcus mutans biofilmsCurrent Organic Chemistry.  22:2664-2670. 2018
2017 Structure-Based Discovery of Small Molecule Inhibitors of Cariogenic VirulenceScientific Reports.  7. 2017
2017 Dietary potassium regulates vascular calcification and arterial stiffnessJCI Insight.  2. 2017
2017 Deficiency of RgpG causes major defects in cell division and biofilm formation, and deficiency of LytR-CpsAPsr family proteins leads to accumulation of cell wall antigens in culture medium by Streptococcus mutansApplied and Environmental Microbiology.  83. 2017
2017 Targeting of Streptococcus mutans Biofilms by a Novel Small Molecule Prevents Dental Caries and Preserves the Oral Microbiome 2017
2017 A commensal streptococcus hijacks a Pseudomonas aeruginosa exopolysaccharide to promote biofilm formationPLoS Pathogens.  13. 2017
2016 Engineering and dissecting the glycosylation pathway of a streptococcal serine-rich repeat adhesinJournal of Biological Chemistry.  291:27354-27363. 2016
2016 Streptococcus mutans copper chaperone, CopZ, is critical for biofilm formation and competitivenessMolecular Oral Microbiology.  31:515-525. 2016
2016 Effects of diadenylate cyclase deficiency on synthesis of extracellular polysaccharide matrix of Streptococcus mutans revisitEnvironmental Microbiology.  18:3612-3619. 2016
2016 Fine-tuned production of hydrogen peroxide promotes biofilm formation of streptococcus parasanguinis by a pathogenic cohabitant Aggregatibacter actinomycetemcomitansEnvironmental Microbiology.  18:4023-4036. 2016
2016 New helical binding domain mediates a glycosyltransferase activity of a bifunctional proteinJournal of Biological Chemistry.  291:22106-22117. 2016
2016 A distinct sortase SrtB anchors and processes a streptococcal adhesin AbpA with a novel structural propertyScientific Reports.  6. 2016
2016 Cyclic di-AMP mediates biofilm formationMolecular Microbiology.  99:945-959. 2016
2016 Nitrite reductase is critical for Pseudomonas aeruginosa survival during co-infection with the oral commensal Streptococcus parasanguinisMicrobiology.  162:376-383. 2016
2016 Structural basis for receptor recognition and pore formation of a zebrafish aerolysin-like proteinEMBO Reports.  17:235-248. 2016
2016 Biomimetic microenvironments for regenerative endodonticsBiomaterials Research.  20. 2016
2016 Hydroxychalcone inhibitors of Streptococcus mutans glucosyl transferases and biofilms as potential anticaries agentsBioorganic and Medicinal Chemistry Letters.  26:3508-3513. 2016
2016 Insights into bacterial protein glycosylation in human microbiota 2016
2016 TLR4 regulates Pulmonary vascular homeostasis and remodeling via redox signalingFrontiers in Bioscience-Landmark.  21:397-409. 2016
2015 A new small molecule inhibits Streptococcus mutans biofilms in vitro and in vivoJournal of Applied Microbiology.  119:1403-1411. 2015
2015 TributeMolecular Oral Microbiology.  30:253-254. 2015
2015 New small-molecule inhibitors of dihydrofolate reductase inhibit Streptococcus mutansInternational Journal of Antimicrobial Agents.  46:174-182. 2015
2015 Glycosyltransferase-mediated sweet modification in oral streptococci 2015
2015 NMR assignment of the amylase-binding protein A from Streptococcus parasanguinisBiomolecular NMR Assignments.  9:173-175. 2015
2015 Inhibition of FOXO1/3 promotes vascular calcification 2015
2015 A conserved domain is crucial for acceptor substrate binding in a family of glucosyltransferasesJournal of Bacteriology.  197:510-517. 2015
2015 Evaluation of ciprofloxacin and metronidazole encapsulated biomimetic nanomatrix gel on Enterococcus faecalis and Treponema denticolaBiomaterials Research.  19. 2015
2015 Kanamycin resistance cassette for genetic manipulation of Treponema denticolaApplied and Environmental Microbiology.  81:4329-4338. 2015
2015 Oral streptococci and nitrite-mediated interference of Pseudomonas aeruginosaInfection and Immunity.  83:101-107. 2015
2014 Disruption of heterotypic community development by Porphyromonas gingivalis with small molecule inhibitorsMolecular Oral Microbiology.  29:185-193. 2014
2014 Antibacterial and antibiofilm activities of makaluvamine analogsMicroorganisms.  2:128-139. 2014
2014 Structure of a novel O-linked N-acetyl-D-glucosamine (O-GlcNAc) transferase, GtfA, reveals insights into the glycosylation of pneumococcal serine-rich repeat adhesinsJournal of Biological Chemistry.  289:20898-20907. 2014
2014 The highly conserved domain of unknown function 1792 has a distinct glycosyltransferase foldNature Communications.  5. 2014
2014 Activation of AKT by O-linked N-Acetylglucosamine induces vascular calcification in diabetes mellitusCirculation Research.  114:1094-1102. 2014
2013 Gap2 promotes the formation of a stable protein complex required for mature Fap1 biogenesisJournal of Bacteriology.  195:2166-2176. 2013
2013 Preliminary X-ray crystallographic studies of an N-terminal domain of unknown function from a putative glycosyltransferase from Streptococcus parasanguinisActa Crystallographica Section F: Structural Biology Communications.  69:520-523. 2013
2013 Structural and biochemical analysis of a bacterial glycosyltransferaseMethods in Molecular Biology.  1022:29-39. 2013
2012 Serum response factor regulates bone formation via IGF-1 and Runx2 signalsJournal of Bone and Mineral Research.  27:1659-1668. 2012
2012 Gap1 functions as a molecular chaperone to stabilize its interactive partner Gap3 during biogenesis of serine-rich repeat bacterial adhesinMolecular Microbiology.  83:866-878. 2012
2012 Structural insight into the role of Streptococcus parasanguinis Fap1 within oral biofilm formationBiochemical and Biophysical Research Communications.  417:421-426. 2012
2011 Canonical SecA associates with an accessory secretory protein complex involved in biogenesis of a Streptococcal serine-rich repeat glycoproteinJournal of Bacteriology.  193:6560-6566. 2011
2011 A molecular chaperone mediates a two-protein enzyme complex and glycosylation of serine-rich streptococcal adhesinsJournal of Biological Chemistry.  286:34923-34931. 2011
2011 New cell surface protein involved in biofilm formation by Streptococcus parasanguinisInfection and Immunity.  79:3239-3248. 2011
2011 Structural and functional analysis of a new subfamily of glycosyltransferases required for glycosylation of serine-rich streptococcal adhesinsJournal of Biological Chemistry.  286:27048-27057. 2011
2011 Calmodulin mediates Fas-induced FADD-independent survival signaling in pancreatic cancer cells via activation of Src-Extracellular Signal-regulated Kinase (ERK)Journal of Biological Chemistry.  286:24776-24784. 2011
2011 A new small molecule specifically inhibits the cariogenic bacterium Streptococcus mutans in multispecies biofilmsAntimicrobial Agents and Chemotherapy.  55:2679-2687. 2011
2010 Purification and characterization of an active N- Acetylglucosaminyltransferase enzyme complex from streptococciApplied and Environmental Microbiology.  76:7966-7971. 2010
2010 RANKL up-regulates brain-type creatine kinase via poly(ADP-ribose) polymerase-1 during osteoclastogenesisJournal of Biological Chemistry.  285:36315-36321. 2010
2010 Structural insights into serine-rich fimbriae from gram-positive bacteriaJournal of Biological Chemistry.  285:32446-32457. 2010
2010 A novel glucosyltransferase is required for glycosylation of a serine-rich adhesin and biofilm formation by Streptococcus parasanguinisJournal of Biological Chemistry.  285:12140-12148. 2010
2009 Study on Fapl glycosylation and maturation regulating by ORF3 coded by fap1-orf4 gene locus of Streptococcus parasanguisZhonghua wei sheng wu xue he mian yi xue za zhi = Zhonghua weishengwuxue he mianyixue zazhi.  29:460-465. 2009
2009 Comprehensive evaluation of Streptococcus sanguinis cell wall-anchored proteins in early infective endocarditisInfection and Immunity.  77:4966-4975. 2009
2009 Glycosylation and biogenesis of a family of serine-rich bacterial adhesinsMicrobiology.  155:317-327. 2009
2008 A conserved C-terminal 13-amino-acid motif of Gap1 Is Required for Gap1 Function and Necessary for the Biogenesis of a Serine-Rich Glycoprotein of Streptococcus parasanguinisInfection and Immunity.  76:5624-5631. 2008
2008 A conserved domain of previously unknown function in Gap1 mediates protein-protein interaction and is required for biogenesis of a serine-rich streptococcal adhesinMolecular Microbiology.  70:1094-1104. 2008
2008 Study on function and interaction of GTF and ORF4 coded by fap1-orf4 gene locus of Streptococcus parasanguisZhonghua wei sheng wu xue he mian yi xue za zhi = Zhonghua weishengwuxue he mianyixue zazhi.  28:771-776. 2008
2008 Phospholipase Cγ2 mediates RANKL-stimulated lymph node organogenesis and osteoclastogenesisJournal of Biological Chemistry.  283:29593-29601. 2008
2008 Identification of critical residues in Gap3 of Streptococcus parasanguinis involved in Fap1 glycosylation, fimbrial formation and in vitro adhesionBMC Microbiology.  8. 2008
2008 Interaction between two putative glycosyltransferases is required for glycosylation of a serine-rich streptococcal adhesinJournal of Bacteriology.  190:1256-1266. 2008
2008 Role of gap3 in Fap1 glycosylation, stability, in vitro adhesion, and fimbrial and biofilm formation of Streptococcus parasanguinisMolecular Oral Microbiology.  23:70-78. 2008
2008 The utility of affinity-tags for detection of a streptococcal protein from a variety of streptococcal speciesJournal of Microbiological Methods.  72:249-256. 2008
2007 Differential roles of individual domains in selection of secretion route of a Streptococcus parasanguinis serine-rich adhesin, Fap1Journal of Bacteriology.  189:7610-7617. 2007
2007 The glycan moieties and the N-terminal polypeptide backbone of a fimbria-associated adhesin, Fap1, play distinct roles in the biofilm development of Streptococcus parasanguinisInfection and Immunity.  75:2181-2188. 2007
2007 Two gene determinants are differentially involved in the biogenesis of Fap1 precursors in Streptococcus parasanguisJournal of Bacteriology.  189:1390-1398. 2007
2006 SecA2 is distinct from SecA in immunogenic specificity, subcellular distribution and requirement for membrane anchoring in Streptococcus parasanguisFEMS Microbiology Letters.  264:174-181. 2006
2006 Inactivation of DNA adenine methyltransferase alters virulence factors in Actinobacillus actinomycetemcomitansMolecular Oral Microbiology.  21:238-244. 2006
2005 A serine-rich glycoprotein of Streptococcus sanguis mediates adhesion to platelets via GPIbBritish Journal of Haematology.  129:101-109. 2005
2004 Investigating the role of secA2 in secretion and glycosylation of a fimbrial adhesin in Streptococcus parasanguis FW213Molecular Microbiology.  53:843-856. 2004
2002 Construction of a novel transposon mutagenesis system useful in the isolation of streptococcus parasanguis mutants defective in Fap1 glycosylationInfection and Immunity.  70:6534-6540. 2002
2002 The Fap1 fimbrial adhesin is a glycoprotein: Antibodies specific for the glycan moiety block the adhesion of Streptococcus parasanguis in an in vitro tooth modelMolecular Microbiology.  43:147-157. 2002
2002 Peptide methionine sulfoxide reductase (MsrA) is not a major virulence determinant for the oral pathogen Actinobacillus actinomycetemcomitansMicrobiology.  148:3695-3703. 2002
2001 Molecular strategies for fimbrial expression and assembly 2001
1999 Identification of dipeptide repeats and a cell wall sorting signal in the fimbriae-associated adhesin, Fap1, of Streptococcus parasanguisMolecular Microbiology.  34:1070-1081. 1999
1998 Isolation and characterization of Fap1, a fimbriae-associated adhesin of Streptococcus parasanguis FW213Molecular Microbiology.  28:487-500. 1998


Year Title Altmetric
2019 Microbial biofilms.  110-114. 2019
2019 Reactive oxygen and nitrogen species in the oral cavity.  33-42. 2019

Principal Investigator On

  • A Novel Glycosylation - Associated Protein is Required for Fimbriae Biogenesis  awarded by National Institute of Dental and Craniofacial Research/NIH/DHHS
  • Amylase Binding Streptococci, Dental Plaque and Caries  awarded by UNIVERSITY OF BUFFALO (STATE UNIVERSITY OF NEW YORK)
  • Amylase Binding and Gene Expression is Streptococcus Gordonii  awarded by UNIVERSITY OF BUFFALO (STATE UNIVERSITY OF NEW YORK)
  • Bacterial Second Messenger Mediated Virulence Regulation in Streptococcus Mutans  awarded by National Institute of Dental and Craniofacial Research/NIH/DHHS
  • Biosynthesis and Function of Treponema Denticola Lipooligosaccharide  awarded by National Institute of Dental and Craniofacial Research/NIH/DHHS
  • BrpA in Virulence Modulation of Streptococcus Mutans  awarded by Louisiana State University
  • Construction of Prokaryotic Expression Clone for Human Amelogenin and Biomimetic Mineralization Ex Vivo  awarded by SICHUAN MEDICAL SCIENCE ACADEMY AND SICHUAN PROVINCIAL PEOPLE'S HOSPITAL
  • Dental Academic Research Training Program (DART)  awarded by National Institute of Dental and Craniofacial Research/NIH/DHHS
  • Dental Academic Research Training Program (DART)  awarded by National Institute of Dental and Craniofacial Research/NIH/DHHS
  • Determination of the Role of Smu.833 in the Fitness and Virulence of Cariogenic Streptococcus Mutans  awarded by National Institute of Dental and Craniofacial Research/NIH/DHHS
  • Development of Small Molecules That Inhibit and Disperse Carciogenic Biofilms  awarded by International Association for Dental Research
  • Dispersion of Streptococcus Mutans Biofilms by a Novel Small Molecule  awarded by National Institute of Dental and Craniofacial Research/NIH/DHHS
  • Genetic Analysis-Adhesion of S Sanguis to S Pellicle  awarded by National Institute of Dental and Craniofacial Research/NIH/DHHS
  • Glycosylation & Function of an Oral Streptococcal Adhesin  awarded by National Institute of Dental and Craniofacial Research/NIH/DHHS
  • Glycosylation and Biogenesis of Streptococcal Adhesins  awarded by National Institute of Dental and Craniofacial Research/NIH/DHHS
  • Glycosylation and Function of an Oral Streptococcal Adhesion  awarded by National Institute of Dental and Craniofacial Research/NIH/DHHS
  • Private Grant  awarded by MICROBIOTIX, INC.
  • Small Molecule Inhibitors of Carciogenic Biofilms  awarded by National Institute of Dental and Craniofacial Research/NIH/DHHS
  • Small Molecule Inhibitors of Cariogenic Biofilms  awarded by National Institute of Dental and Craniofacial Research/NIH/DHHS
  • Education And Training

  • Doctor of Philosophy in Cell / Cellular and Molecular Biology, University of Vermont 1999
  • Full Name

  • Hui Wu