Positions

Overview

  • Following his postdoctoral fellowship at the University of Rochester, Dr. Graves joined the faculty in the Department of Chemistry and Biochemistry at the University of Mississippi in 1984. He rose through the ranks of assistant, associate, and full professor at the University of Mississippi and was awarded the title of Distinguished Faculty Fellow from the University of Mississippi College of Liberal Arts in 2001. In 2003, Dr. Graves was recruited to become the chair of the Department of Chemistry at the University of Alabama at Birmingham. He served as chair of the department from May 2003 to July 2015.
  • Selected Publications

    Academic Article

    Year Title Altmetric
    2018 Encapsulation and ultrasound-triggered release of G-Quadruplex DNA in multilayer hydrogel microcapsulesPolymers.  10. 2018
    2018 Stability of the Na+ Form of the Human Telomeric G-Quadruplex: Role of Adenines in Stabilizing G-Quadruplex StructureACS Omega.  3:844-855. 2018
    2016 Tandem DART™ MS methods for methadone analysis in unprocessed urineJournal of Analytical Toxicology.  40:140-147. 2016
    2016 Telomeric G-quadruplex-forming DNA fragments induce TLR9-mediated and LL-37-regulated invasion in breast cancer cells in vitroBreast Cancer Research and Treatment.  155:261-271. 2016
    2014 Recognition and binding of human telomeric G-quadruplex DNA by unfolding protein 1Biochemistry.  53:3347-3356. 2014
    2014 Addition of bases to the 5′-end of human telomeric DNA: Influences on thermal stability and energetics of unfoldingMolecules.  19:2286-2298. 2014
    2014 Hypoxia regulates Toll-like receptor-9 expression and invasive function in human brain cancer cells in vitroOncology Letters.  8:266-274. 2014
    2013 DNA from dead cancer cells induces TLR9-mediated invasion and inflammation in living cancer cellsBreast Cancer Research and Treatment.  142:477-487. 2013
    2013 Short DNA sequences and bacterial DNA induce esophageal, gastric, and colorectal cancer cell invasionActa pathologica et microbiologica Scandinavica.  121:511-522. 2013
    2012 Low TLR9 expression defines an aggressive subtype of triple-negative breast cancerBreast Cancer Research and Treatment.  135:481-493. 2012
    2012 Amsacrine as a topoisomerase II poison: Importance of drug-DNA interactionsBiochemistry.  51:1730-1739. 2012
    2012 Drug interactions with bacillus anthracis topoisomerase IV: Biochemical basis for quinolone action and resistanceBiochemistry.  51:370-381. 2012
    2011 Contributions of the D-ring to the activity of etoposide against human topoisomerase IIα: Potential interactions with DNA in the ternary enzyme-drug-DNA complexBiochemistry.  50:5058-5066. 2011
    2011 Interactions between the etoposide derivative F14512 and human type II topoisomerases: Implications for the C4 spermine moiety in promoting enzyme-mediated DNA cleavageBiochemistry.  50:3240-3249. 2011
    2009 Interactions of actinomycin D with human telomeric G-quadruplex DNABiochemistry.  48:4440-4447. 2009
    2008 Toll-like receptor 9 mediates CpG oligonucleotide-induced cellular invasion 2008
    2008 Substituents on etoposide that interact with human topoisomerase IIα in the binary enzyme-drug complex: Contributions to etoposide binding and activityBiochemistry.  47:4501-4509. 2008
    2008 Antiviral and anticancer optimization studies of the DNA-binding marine natural product aaptamine 2008
    2007 Topoisomerase II-drug interaction domains: Identification of substituents on etoposide that interact with the enzymeBiochemistry.  46:8217-8225. 2007
    2004 Extending nature's leads: The anticancer agent ellipticine 2004
    2004 Influence of the amino substituents in the interaction of ethidium bromide with DNABiophysical Journal.  87:3974-3981. 2004
    2003 Influence of Substituent Modifications on DNA Binding Energetics of Acridine-Based Anticancer AgentsBiochemistry.  42:13754-13761. 2003
    2001 An unusual oxidation of a benzylic methylene group by thionyl chloride: A synthesis of 1,3-dihydro-2-[2-(dimethyl-amino)ethyl]-1,3-dioxopyrrolo[3,4-c]acridine derivativesJournal of Organic Chemistry.  66:612-616. 2001
    2001 Drug-DNA interactions.Methods in Molecular Biology.  95:161-169. 2001
    2001 Targeting DNA through covalent interactions of reversible binding drugsMethods in Enzymology.  340:377-395. 2001
    2000 Intercalative binding of small molecules to nucleic acidsCurrent Organic Chemistry.  4:915-929. 2000
    1999 DNA topoisomerases as targets for the anticancer drug TAS-103: DNA interactions and topoisomerase catalytic inhibitionBiochemistry.  38:15580-15586. 1999
    1999 5,7-Disubstituted analogues of the mixed topoisomerase I/II poison N-[2-(dimethylamino)ethyl]acridine-4-carboxamide (DACA): DNA binding and patterns of cytotoxicity 1999
    1999 Calories from carbohydrates: Energetic contribution of the carbohydrate moiety of rebeccamycin to DNA binding and the effect of its orientation on topoisomerase I inhibition 1999
    1998 Chemical cross-linking of ethidium to DNA by glyoxal 1998
    1997 Covalent attachment of ethidium to DNA results in enhanced topoisomerase II-mediated DNA cleavageBiochemistry.  36:15884-15891. 1997
    1996 Covalent interactions of ethidium and actinomycin D with nucleic acids. Photoaffinity labeling of DNAAdvances in DNA Sequence-Specific Agents.  2:169-186. 1996
    1995 Photoaffinity approaches to determining the sequence selectivities of DNA-small molecule interactions: Actinomycin D and ethidiumNucleic Acids Research.  23:1252-1259. 1995
    1995 Interactions of Acridine Antitumor Agents with DNA: Binding Energies and Groove PreferencesBiochemistry.  34:13682-13687. 1995
    1994 Daunomycin modifies the sequence-selective recognition of DNA by actinomycinNucleic Acids Research.  22:5241-5246. 1994
    1994 Binding of Actinomycin D to the T(G)nT Motif of Double-Stranded DNA: Determination of the Guanine Requirement in Nonclassical, Non-GpC Binding SitesBiochemistry.  33:11493-11500. 1994
    1994 Use of a Photoactive Derivative of Actinomycin To Investigate Shuffling between Binding Sites on DNABiochemistry.  33:8736-8745. 1994
    1993 Dissection of the Free Energy of Anthracycline Antibiotic Binding to DNA: Electrostatic ContributionsJournal of the American Chemical Society.  115:5360-5364. 1993
    1993 Influence of DNA Base Sequence on the Binding Energetics of Actinomycin DBiochemistry.  32:5881-5887. 1993
    1992 Structure-activity studies of amsacrine analogs in drug resistant human leukemia cell lines expressing either altered DNA topoisomerase ii or P-glycoprotein 1992
    1991 Inhibition of the B to Z Transition in Poly(dGdC)·Poly(dGdC) by Covalent Attachment of Ethidium: Equilibrium StudiesBiochemistry.  30:10925-10931. 1991
    1991 Inhibition of the B to Z Transition in Poly(dGdC)·Poly(dGdC) by Covalent Attachment of Ethidium: Kinetic StudiesBiochemistry.  30:10931-10937. 1991
    1991 Interactions of Anilinoacridines with Nucleic Acids: Effects of Substituent Modifications on DNA-Binding PropertiesBiochemistry.  30:4277-4283. 1991
    1989 Thermodynamics of the interactions of m-AMSA and o-AMSA with nucleic acids: Influence of ionic strength and DNA base compositionNucleic Acids Research.  17:9933-9946. 1989
    1989 7-Azidoactinomycin D: A novel probe for examining actinomycin D-DNA interactionsJournal of Biological Chemistry.  264:7262-7266. 1989
    1989 7-azido-actinomycin d: A photoaffinity probe of the sequence specificity of DNA binding by actinomycin dJournal of Biomolecular Structure and Dynamics.  7:591-605. 1989
    1989 INTRAMOLECULAR FLUORESCENCE QUENCHING IN COVALENT ACRYLAMIDE‐INDOLE ADDUCTSPhotochemistry and Photobiology.  49:725-729. 1989
    1989 Two-Dimensional NMR Studies on the Anthramycin-d(ATGCAT)2 AdductBiochemistry.  28:9988-9994. 1989
    1988 Cooperative binding of m-AMSA to nucleic acidsNucleic Acids Research.  16:9707-9719. 1988
    1988 Carcinogennucleic acid interactions:Equilibrium binding studies of aflatoxins b1 and b2Journal of Biomolecular Structure and Dynamics.  5:1025-1041. 1988
    1985 Structure of the Anthramycin-d(ATGCAT)2 Adduct from One- and Two-Dimensional Proton NMR Experiments in Solution†Biochemistry.  24:7573-7581. 1985
    1985 NMR analysis of an oligodeoxyribonucleotide-drug adduct: anthramycin-d(ATGCAT).Progress in clinical and biological research.  172 B:193-205. 1985
    1984 The reaction of anthramycin with DNA. Proton and carbon nuclear magnetic resonance studies on the structure of the anthramycin-DNA adductJournal of Biological Chemistry.  259:8202-8209. 1984
    1983 Single-cell partition analysis-A direct fluorescence technique for examining ligand-macromolecule interactionsAnalytical Biochemistry.  134:73-81. 1983
    1983 Adriamycin and Daunorubicin Bind in a Cooperative Manner to Deoxyribonucleic AcidBiochemistry.  22:3941-3947. 1983
    1983 Synthesis and characterization of ethidium analogs: Emphasis on amino and azido substituents 1983
    1982 Cooperative binding of adriamycin and daunorubicin to nucleic acidsThe FASEB Journal.  41. 1982
    1981 Solute enhanced partition analysis-A novel method for measuring the binding of drugs to DNABiochemical and Biophysical Research Communications.  98:317-323. 1981
    1981 Ethidium Bromide and Its Photoreactive Analogues: Spectroscopic Analysis of Deoxyribonucleic Acid Binding PropertiesBiochemistry.  20:1887-1892. 1981
    1980 Erratum: Comparative studies of the binding of ethidium bromide and its photoreactive analogues to nucleic acids by fluorescence and rapid kinetics (Biochemistry (1980) 19, 14, (3221-3226))Biochemistry.  19:6216. 1980
    1980 Comparative Studies of the Binding of Ethidium Bromide and its Photoreactive Analogs to Nucleic Acids by Fluorescence and Rapid KineticsBiochemistry.  19:3221-3226. 1980
    1979 Covalent binding of ethidium azide analogs to Salmonella DNA in vivo: Competition by ethidium bromideBiochemical and Biophysical Research Communications.  87:424-432. 1979
    1979 Characterization of the mutagenic ethidium azide interactions with deoxyribonucleic acidsThe FASEB Journal.  38. 1979
    1979 Ethidium bromide enhancement of frameshift mutagenesis caused by photoactivatable ethidium analogsMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis.  63:225-232. 1979
    1978 Crystal structure of ethidium monoazide, a photoactive compound that reacts with nucleic acidsJournal of Chemical Crystallography.  8:93-103. 1978
    1978 Covalent binding of ethidium azide analogs to DNA: competition by ethidiumThe FASEB Journal.  37. 1978
    1977 Synthesis, separation and characterization of the mono- and diazide analogs of ethidium bromideBiochimica et Biophysica Acta - Section Nucleic Acids And Protein Synthesis.  479:98-104. 1977

    Research Overview

  • The focus of my research involves the biophysical characterization of nucleic acid structures and stabilities as related to both base sequence effects as well as complex formation with small molecules and proteins. My initial research efforts focused on DNA as a target for small molecules such as anticancer agents including intercalating and groove binders and the mechanistic processes of molecular recognition. An understanding of the forces that govern DNA base sequence-specific interactions for small molecules and/or proteins is fundamental towards our comprehension of basic processes associated with gene expression such as transcription, recombination, DNA repair, as well as the recognition of DNA cleavage sites for nuclear enzymes such as topoisomerase I and topoisomerase II.

    More recently, our research has focused on the structural, stability, and protein-binding properties of G-quadruplex DNA fragments. Recent studies in my laboratory have demonstrated that small deoxyribonucleotides can act as modulators of a number of toll-like receptor (TLR) mediated biological responses, including cancer cell invasion. Although the nature of the TLR9-mediated cell invasion is not understood, the key determinant seems to reside in the stable nuclease-resistant structure(s) of the oligonucleotides either for binding to the target protein or protection from nuclease invasion. We continue to use a multifaceted approach of calorimetry and high-resolution NMR to discern the structure and stability of nucleic acids and protein-nucleic acid complexes.
  • Teaching Overview

  • Research Methods in Chemistry and Biochemistry, Concepts in Biochemistry, Advanced Biochemistry, Biophysical Chemistry, Biochemistry Laboratory
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  • Education And Training

  • Doctor of Philosophy in Biochemistry, University of Alabama at Birmingham 1979
  • Bachelor of Science or Mathematics in Chemistry, University of Alabama at Birmingham 1974
  • Full Name

  • David Graves