Positions

Selected Publications

Academic Article

Year Title Altmetric
2021 Stability Prediction for Mutations in the Cytosolic Domains of Cystic Fibrosis Transmembrane Conductance Regulator 2021
2018 Ligand binding to a remote site thermodynamically corrects the F508del mutation in the human cystic fibrosis transmembrane conductance regulator.  Journal of Biological Chemistry.  293:17685-17704. 2018
2018 Structural stability of purified human CFTR is systematically improved by mutations in nucleotide binding domain 1 2018
2017 Stabilization of a nucleotide-binding domain of the cystic fibrosis transmembrane conductance regulator yields insight into disease-causing mutations.  Journal of Biological Chemistry.  292:14147-14164. 2017
2017 Direct binding of the Corrector VX-809 to Human CFTR NBD1: Evidence of an Allosteric coupling between the Binding site and the NBD1:CL4 Interface s.  Molecular Pharmacology.  92:124-135. 2017
2017 Interactions and cooperativity between P-glycoprotein structural domains determined by thermal unfolding provides insights into its solution structure and function 2017
2016 A Guide to Differential Scanning Calorimetry of Membrane and Soluble Proteins in Detergents.  Methods in Enzymology.  567:319-358. 2016
2015 Restoration of NBD1 thermal stability is necessary and sufficient to correct Δf508 CFTR folding and assembly.  Journal of Molecular Biology.  427:106-120. 2015
2014 Membrane protein stability can be compromised by detergent interactions with the extramembranous soluble domains.  Protein Science.  23:769-789. 2014
2012 Conformational changes relevant to channel activity and folding within the first nucleotide binding domain of the cystic fibrosis transmembrane conductance regulator.  Journal of Biological Chemistry.  287:28480-28494. 2012
2011 A gene optimization strategy that enhances production of fully functional P-Glycoprotein in Pichia pastoris.  PLoS ONE.  6. 2011
2010 Integrated biophysical studies implicate partial unfolding of NBD1 of CFTR in the molecular pathogenesis of F508del cystic fibrosis.  Protein Science.  19:1932-1947. 2010
2010 Thermal unfolding studies show the disease causing F508del mutation in CFTR thermodynamically destabilizes nucleotide-binding domain 1.  Protein Science.  19:1917-1931. 2010
2010 Structures of a minimal human CFTR first nucleotide-binding domain as a monomer, head-to-tail homodimer, and pathogenic mutant 2010
2009 SAR studies for a new class of antibacterial NAD biosynthesis inhibitors.  ACS Combinatorial Science.  11:617-625. 2009
2009 Virtual screening to identify lead inhibitors for bacterial NAD synthetase (NADs).  Bioorganic and Medicinal Chemistry Letters.  19:2001-2005. 2009
2008 Structure of nicotinic acid mononucleotide adenylyltransferase from Bacillus anthracis 2008
2007 Structural adaptation of an interacting non-native C-terminal helical extension revealed in the crystal structure of NAD+ synthetase from Bacillus anthracis.  Acta Crystallographica Section D: Biological Crystallography.  63:891-905. 2007
2007 Antibacterial nicotinamide adenine dinucleotide synthetase inhibitors: Amide- and ether-linked tethered dimers with α-amino acid end groups.  Journal of Medicinal Chemistry.  50:2612-2621. 2007
2007 His-tag impact on structure.  Acta Crystallographica Section D: Biological Crystallography.  63:295-301. 2007
2005 Förster resonance energy transfer measurements are consistent with a helical bundle model for lipid-free apolipoprotein A-I.  Biochemistry.  44:16413-16425. 2005
2005 Engineering mouse apolipoprotein A-I into a monomeric, active protein useful for structural determination.  Biochemistry.  44:14907-14919. 2005
2005 Tethered dimer inhibitors of NAD synthetase: Parallel synthesis of an aryl-substituted SAR library.  ACS Combinatorial Science.  7:898-904. 2005
2004 Role of inhibitor aliphatic chain in the thermodynamics of inhibitor binding to Escherichia coli enoyl-ACP reductase and the Phe203Leu mutant: A proposed mechanism for drug resistance.  Biochemistry.  43:13380-13389. 2004
2004 Dimethyl sulfoxide at 2.5% (v/v) alters the structural cooperativity and unfolding mechanism of dimeric bacterial NAD+ synthetase.  Protein Science.  13:830-841. 2004
2003 Deletion of DnaK's lid strengthens binding to the nucleotide exchange factor, GrpE: A kinetic and thermodynamic analysis.  Biochemistry.  42:9028-9040. 2003
2003 Tethered dimers as NAD synthetase inhibitors with antibacterial activity.  Journal of Medicinal Chemistry.  46:3371-3381. 2003
2002 NH3-dependent NAD+ synthetase from Bacillus subtilis at 1 Å resolution.  Acta Crystallographica Section D: Biological Crystallography.  58:1138-1146. 2002
2001 Kinetic and calorimetric evidence for two distinct scaffolding protein binding populations within the bacteriophage P22 procapsid.  Biochemistry.  40:8962-8970. 2001
2001 Stabilization of active-site loops in NH3-dependent NAD+ synthetase from Bacillus subtilis.  Acta Crystallographica Section D: Biological Crystallography.  57:806-812. 2001
2001 Structural models of human apolipoprotein A-I: A critical analysis and review 2001
2000 Stereoselective binding of an enantiomeric pair of stromelysin-1 inhibitors caused by conformational entropy factors.  Bioorganic and Medicinal Chemistry Letters.  10:2427-2430. 2000
2000 Down-regulation of cell surface receptors is modulated by polar residues within the transmembrane domain 2000
1999 Human apolipoprotein A-I: Structure determination and analysis of unusual diffraction characteristics.  Acta Crystallographica Section D: Biological Crystallography.  55:2013-2021. 1999
1999 Analysis of the binding of hydroxamic acid and carboxylic acid inhibitors to the stromelysin-1 (matrix metalloproteinase-3) catalytic domain by isothermal titration calorimetry.  Biochemistry.  38:13592-13601. 1999
1999 Crystallization of truncated human apolipoprotein A-I in a novel conformation.  Acta Crystallographica Section D: Biological Crystallography.  55:1578-1583. 1999
1999 The structure of human lipoprotein A-I. Evidence for the 'belt' model.  Journal of Biological Chemistry.  274:14541-14544. 1999
1998 The lipid-free structure of apolipoprotein A-I: Effects of amino- terminal deletions.  Biochemistry.  37:11714-11725. 1998
1998 Structural analysis of apolipoprotein A-I: Effects of amino- and carboxy-terminal deletions on the lipid-free structure.  Biochemistry.  37:945-955. 1998
1997 Crystal structure of truncated human apolipoprotein A-I suggests a lipid-bound conformation 1997
1997 Structural analysis of apolipoprotein A-I: Limited proteolysis of methionine-reduced and -oxidized lipid-free and lipid-bound human apo A-I.  Biochemistry.  36:7615-7624. 1997
1997 Truncation of the amine terminus of human apolipoprotein A-I substantially alters only the lipid-free conformation.  Biochemistry.  36:288-300. 1997
1996 Kinetic and structural analysis of mutant CD4 receptors that are defective in HIV gp120 binding 1996
1996 Storage stability of the solution formulation of sCD4 determined by DSC in comparison with two functional assays 1996
1995 Structural models of human apolipoprotein A-I.  BBA - Lipids and Lipid Metabolism.  1256:103-129. 1995
1995 Interdomain Communication of T-Cell CD4 Studied by Absorbance and Fluorescence Difference Spectroscopy Measurements of Urea-Induced Unfolding.  Biochemistry.  34:6464-6474. 1995
1994 The amphipathic α helix: A multifunctional structural motif in plasma apolipoproteins.  Advances in Protein Chemistry.  45:303-369. 1994
1993 Intracellular transport and virion incorporation of vpx requires interaction with other virus type-specific components.  Virology.  193:222-233. 1993
1992 Analysis of the Two-State Behavior of the Thermal Unfolding of Serum Retinol Binding Protein Containing a Single Retinol Ligand.  Biochemistry.  31:5560-5567. 1992
1992 The amphipathic helix in the exchangeable apolipoproteins: A review of secondary structure and function.  Journal of Lipid Research.  33:141-166. 1992
1992 Thermal stabilities of mutant Escherichia coli tryptophan synthase α subunits.  Archives of Biochemistry and Biophysics.  292:34-41. 1992
1991 Role of amphipathic helixes in HDL structure/function.  Advances in human genetics.  285:131-140. 1991
1990 Amphipathic helix motif: Classes and properties 1990
1990 Use of synthetic peptide analogues to localize lecithin:cholesterol acyltransferase activating domain in apolipoprotein A-I.  Arteriosclerosis (Dallas, Tex.).  10:95-105. 1990
1989 Characterization of apoA-I-containing lipoprotein subpopulations secreted by HepG2 cells.  Journal of Lipid Research.  30:1429-1436. 1989
1989 Structure and thermal stability of monomeric bacteriorhodopsin in mixed pospholipid/detergent micelles 1989
1987 Characterization of high density lipoprotein subspecies: Structural studies by single vertical spin ultracentrifugation and immunoaffinity chromatography.  Journal of Lipid Research.  28:913-929. 1987
1987 pH Dependence of Bacteriorhodopsin Thermal Unfolding.  Biochemistry.  26:7431-7438. 1987
1985 Studies of synthetic peptide analogs of the amphiphatic helix. Correlation of structure with function.  Journal of Biological Chemistry.  260:10256-10262. 1985
1985 Studies of synthetic peptide analogs of the amphiphatic helix. Structure of complexes with dimyristoyl phosphatidylcholine.  Journal of Biological Chemistry.  260:10248-10255. 1985
1984 Structural Studies of Apolipoprotein A-I/Phosphatidylcholine Recombinants by High-Field Proton NMR, Nondenaturing Gradient Gel Electrophoresis, and Electron Microscopy.  Biochemistry.  23:359-367. 1984
1983 Studies of synthetic peptide analogs of the amphipathic helix. Competitive displacement of exchangeable apolipoproteins from native lipoproteins.  Journal of Biological Chemistry.  258:2290-2295. 1983
1982 Structural domains of vesicular stomatitis virus. A study by differential scanning calorimetry, thermal gel analysis, and thermal electron microscopy..  Journal of Biological Chemistry.  257:12-15. 1982
1982 Dynamics of Apolipoprotein-Phospholipid Interactions.  Biophysical Journal.  37:172-173. 1982
1982 Minimal Size Phosphatidylcholine Vesicles: Effects of Radius of Curvature on Head Group Packing and Conformation.  Biochemistry.  21:4569-4575. 1982
1982 Structural Domains in Vesicular Stomatitis Virus Membrane as Studied by Differential Scanning Calorimetry.  Biophysical Journal.  37:25-26. 1982

Education And Training

  • Doctor of Philosophy in Medicinal and Pharmaceutical Chemistry, University of Kansas 1979

    • Full Name

  • Christie Brouillette