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Christie Brouillette Ph.D.
Professor Emeritus
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christie@uab.edu
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Christie Brouillette Ph.D.
Professor Emeritus
Positions
Senior Scientist (C)
,
O'Neal Comprehensive Cancer Center
,
School of Medicine
Senior Scientist (C)
,
Cystic Fibrosis Research Center
,
School of Medicine
Senior Scientist
,
Experimental Therapeutics
,
O'Neal Comprehensive Cancer Center
Professor - Emeritus (P)
,
Biochemistry & Molecular Genetics
,
Academic Joint Departments
Publications
Research
Background
Contact
Publications
Selected Publications
Academic Article
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Year
Title
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2021
Stability Prediction for Mutations in the Cytosolic Domains of Cystic Fibrosis Transmembrane Conductance Regulator
2021
2018
Ligand binding to a remote site thermodynamically corrects the F508del mutation in the human cystic fibrosis transmembrane conductance regulator
.
Journal of Biological Chemistry
. 293:17685-17704.
2018
2018
Structural stability of purified human CFTR is systematically improved by mutations in nucleotide binding domain 1
2018
2017
Stabilization of a nucleotide-binding domain of the cystic fibrosis transmembrane conductance regulator yields insight into disease-causing mutations
.
Journal of Biological Chemistry
. 292:14147-14164.
2017
2017
Direct binding of the Corrector VX-809 to Human CFTR NBD1: Evidence of an Allosteric coupling between the Binding site and the NBD1:CL4 Interface
s
.
Molecular Pharmacology
. 92:124-135.
2017
2017
Interactions and cooperativity between P-glycoprotein structural domains determined by thermal unfolding provides insights into its solution structure and function
2017
2016
A Guide to Differential Scanning Calorimetry of Membrane and Soluble Proteins in Detergents
.
Methods in Enzymology
. 567:319-358.
2016
2015
Restoration of NBD1 thermal stability is necessary and sufficient to correct Δf508 CFTR folding and assembly
.
Journal of Molecular Biology
. 427:106-120.
2015
2014
Membrane protein stability can be compromised by detergent interactions with the extramembranous soluble domains
.
Protein Science
. 23:769-789.
2014
2012
Conformational changes relevant to channel activity and folding within the first nucleotide binding domain of the cystic fibrosis transmembrane conductance regulator
.
Journal of Biological Chemistry
. 287:28480-28494.
2012
2011
A gene optimization strategy that enhances production of fully functional P-Glycoprotein in Pichia pastoris
.
PLoS ONE
. 6.
2011
2010
Integrated biophysical studies implicate partial unfolding of NBD1 of CFTR in the molecular pathogenesis of F508del cystic fibrosis
.
Protein Science
. 19:1932-1947.
2010
2010
Thermal unfolding studies show the disease causing F508del mutation in CFTR thermodynamically destabilizes nucleotide-binding domain 1
.
Protein Science
. 19:1917-1931.
2010
2010
Structures of a minimal human CFTR first nucleotide-binding domain as a monomer, head-to-tail homodimer, and pathogenic mutant
2010
2009
SAR studies for a new class of antibacterial NAD biosynthesis inhibitors
.
ACS Combinatorial Science
. 11:617-625.
2009
2009
Virtual screening to identify lead inhibitors for bacterial NAD synthetase (NADs)
.
Bioorganic and Medicinal Chemistry Letters
. 19:2001-2005.
2009
2008
Structure of nicotinic acid mononucleotide adenylyltransferase from Bacillus anthracis
2008
2007
Structural adaptation of an interacting non-native C-terminal helical extension revealed in the crystal structure of NAD
+
synthetase from Bacillus anthracis
.
Acta Crystallographica Section D: Biological Crystallography
. 63:891-905.
2007
2007
Antibacterial nicotinamide adenine dinucleotide synthetase inhibitors: Amide- and ether-linked tethered dimers with α-amino acid end groups
.
Journal of Medicinal Chemistry
. 50:2612-2621.
2007
2007
His-tag impact on structure
.
Acta Crystallographica Section D: Biological Crystallography
. 63:295-301.
2007
2005
Förster resonance energy transfer measurements are consistent with a helical bundle model for lipid-free apolipoprotein A-I
.
Biochemistry
. 44:16413-16425.
2005
2005
Engineering mouse apolipoprotein A-I into a monomeric, active protein useful for structural determination
.
Biochemistry
. 44:14907-14919.
2005
2005
Tethered dimer inhibitors of NAD synthetase: Parallel synthesis of an aryl-substituted SAR library
.
ACS Combinatorial Science
. 7:898-904.
2005
2004
Role of inhibitor aliphatic chain in the thermodynamics of inhibitor binding to Escherichia coli enoyl-ACP reductase and the Phe203Leu mutant: A proposed mechanism for drug resistance
.
Biochemistry
. 43:13380-13389.
2004
2004
Dimethyl sulfoxide at 2.5% (v/v) alters the structural cooperativity and unfolding mechanism of dimeric bacterial NAD
+
synthetase
.
Protein Science
. 13:830-841.
2004
2003
Deletion of DnaK's lid strengthens binding to the nucleotide exchange factor, GrpE: A kinetic and thermodynamic analysis
.
Biochemistry
. 42:9028-9040.
2003
2003
Tethered dimers as NAD synthetase inhibitors with antibacterial activity
.
Journal of Medicinal Chemistry
. 46:3371-3381.
2003
2002
NH
3
-dependent NAD
+
synthetase from Bacillus subtilis at 1 Å resolution
.
Acta Crystallographica Section D: Biological Crystallography
. 58:1138-1146.
2002
2001
Kinetic and calorimetric evidence for two distinct scaffolding protein binding populations within the bacteriophage P22 procapsid
.
Biochemistry
. 40:8962-8970.
2001
2001
Stabilization of active-site loops in NH
3
-dependent NAD
+
synthetase from Bacillus subtilis
.
Acta Crystallographica Section D: Biological Crystallography
. 57:806-812.
2001
2001
Structural models of human apolipoprotein A-I: A critical analysis and review
2001
2000
Stereoselective binding of an enantiomeric pair of stromelysin-1 inhibitors caused by conformational entropy factors
.
Bioorganic and Medicinal Chemistry Letters
. 10:2427-2430.
2000
2000
Down-regulation of cell surface receptors is modulated by polar residues within the transmembrane domain
2000
1999
Human apolipoprotein A-I: Structure determination and analysis of unusual diffraction characteristics
.
Acta Crystallographica Section D: Biological Crystallography
. 55:2013-2021.
1999
1999
Analysis of the binding of hydroxamic acid and carboxylic acid inhibitors to the stromelysin-1 (matrix metalloproteinase-3) catalytic domain by isothermal titration calorimetry
.
Biochemistry
. 38:13592-13601.
1999
1999
Crystallization of truncated human apolipoprotein A-I in a novel conformation
.
Acta Crystallographica Section D: Biological Crystallography
. 55:1578-1583.
1999
1999
The structure of human lipoprotein A-I. Evidence for the 'belt' model
.
Journal of Biological Chemistry
. 274:14541-14544.
1999
1998
The lipid-free structure of apolipoprotein A-I: Effects of amino- terminal deletions
.
Biochemistry
. 37:11714-11725.
1998
1998
Structural analysis of apolipoprotein A-I: Effects of amino- and carboxy-terminal deletions on the lipid-free structure
.
Biochemistry
. 37:945-955.
1998
1997
Crystal structure of truncated human apolipoprotein A-I suggests a lipid-bound conformation
1997
1997
Structural analysis of apolipoprotein A-I: Limited proteolysis of methionine-reduced and -oxidized lipid-free and lipid-bound human apo A-I
.
Biochemistry
. 36:7615-7624.
1997
1997
Truncation of the amine terminus of human apolipoprotein A-I substantially alters only the lipid-free conformation
.
Biochemistry
. 36:288-300.
1997
1996
Kinetic and structural analysis of mutant CD4 receptors that are defective in HIV gp120 binding
1996
1996
Storage stability of the solution formulation of sCD4 determined by DSC in comparison with two functional assays
1996
1995
Structural models of human apolipoprotein A-I
.
BBA - Lipids and Lipid Metabolism
. 1256:103-129.
1995
1995
Interdomain Communication of T-Cell CD4 Studied by Absorbance and Fluorescence Difference Spectroscopy Measurements of Urea-Induced Unfolding
.
Biochemistry
. 34:6464-6474.
1995
1994
The amphipathic α helix: A multifunctional structural motif in plasma apolipoproteins
.
Advances in Protein Chemistry
. 45:303-369.
1994
1993
Intracellular transport and virion incorporation of vpx requires interaction with other virus type-specific components
.
Virology
. 193:222-233.
1993
1992
Analysis of the Two-State Behavior of the Thermal Unfolding of Serum Retinol Binding Protein Containing a Single Retinol Ligand
.
Biochemistry
. 31:5560-5567.
1992
1992
The amphipathic helix in the exchangeable apolipoproteins: A review of secondary structure and function
.
Journal of Lipid Research
. 33:141-166.
1992
1992
Thermal stabilities of mutant Escherichia coli tryptophan synthase α subunits
.
Archives of Biochemistry and Biophysics
. 292:34-41.
1992
1991
Role of amphipathic helixes in HDL structure/function
.
Advances in human genetics
. 285:131-140.
1991
1990
Amphipathic helix motif: Classes and properties
1990
1990
Use of synthetic peptide analogues to localize lecithin:cholesterol acyltransferase activating domain in apolipoprotein A-I
.
Arteriosclerosis (Dallas, Tex.)
. 10:95-105.
1990
1989
Characterization of apoA-I-containing lipoprotein subpopulations secreted by HepG2 cells
.
Journal of Lipid Research
. 30:1429-1436.
1989
1989
Structure and thermal stability of monomeric bacteriorhodopsin in mixed pospholipid/detergent micelles
1989
1987
Characterization of high density lipoprotein subspecies: Structural studies by single vertical spin ultracentrifugation and immunoaffinity chromatography
.
Journal of Lipid Research
. 28:913-929.
1987
1987
pH Dependence of Bacteriorhodopsin Thermal Unfolding
.
Biochemistry
. 26:7431-7438.
1987
1985
Studies of synthetic peptide analogs of the amphiphatic helix. Correlation of structure with function
.
Journal of Biological Chemistry
. 260:10256-10262.
1985
1985
Studies of synthetic peptide analogs of the amphiphatic helix. Structure of complexes with dimyristoyl phosphatidylcholine
.
Journal of Biological Chemistry
. 260:10248-10255.
1985
1984
Structural Studies of Apolipoprotein A-I/Phosphatidylcholine Recombinants by High-Field Proton NMR, Nondenaturing Gradient Gel Electrophoresis, and Electron Microscopy
.
Biochemistry
. 23:359-367.
1984
1983
Studies of synthetic peptide analogs of the amphipathic helix. Competitive displacement of exchangeable apolipoproteins from native lipoproteins
.
Journal of Biological Chemistry
. 258:2290-2295.
1983
1982
Structural domains of vesicular stomatitis virus. A study by differential scanning calorimetry, thermal gel analysis, and thermal electron microscopy.
.
Journal of Biological Chemistry
. 257:12-15.
1982
1982
Dynamics of Apolipoprotein-Phospholipid Interactions
.
Biophysical Journal
. 37:172-173.
1982
1982
Minimal Size Phosphatidylcholine Vesicles: Effects of Radius of Curvature on Head Group Packing and Conformation
.
Biochemistry
. 21:4569-4575.
1982
1982
Structural Domains in Vesicular Stomatitis Virus Membrane as Studied by Differential Scanning Calorimetry
.
Biophysical Journal
. 37:25-26.
1982
Research
Principal Investigator On
CFTR Structural Energetics: Domain Communication, Ligand Binding Response
awarded by
Cystic Fibrosis Foundation ^
CFTR Structural Energetics: Domain Communication, Ligand Binding Response
awarded by
Cystic Fibrosis Foundation ^
Discovery and Preclinical Development of Drugs for Anthrax, Plague and Tularemia
awarded by
National Institute of Allergy and Infectious Diseases/NIH/DHHS
Expression and Purification of CFTR Domain Constructs
awarded by
Cystic Fibrosis Foundation ^
MicroCal Auto-iTC200; Automated High Sensitivity Isothermal Titration Calorimetry for Multiple Uses
awarded by
National Center for Research Resources/NIH/DHHS
Recombinant CFTR: Cooperativity and Structural Domains
awarded by
Cystic Fibrosis Foundation ^
Investigator On
A Comprehensive Evaluation of Microgravity Protein Crystallization
awarded by
NASA - National Aeronautics & Space Administration
Comprehensive Cancer Center Core Support Grant
awarded by
National Cancer Institute/NIH/DHHS
Production & Crystallization of Membrane Protein for 3D Structure
awarded by
National Institute of General Medical Sciences/NIH/DHHS
Background
Education And Training
Doctor of Philosophy in Medicinal and Pharmaceutical Chemistry,
University of Kansas
1979
Contact
Full Name
Christie
Brouillette