Selected Publications

Academic Article

Year Title Altmetric
2018 Ligand binding to a remote site thermodynamically corrects the F508del mutation in the human cystic fibrosis transmembrane conductance regulatorJournal of Biological Chemistry.  293:17685-17704. 2018
2018 Structural stability of purified human CFTR is systematically improved by mutations in nucleotide binding domain 1 2018
2017 Stabilization of a nucleotide-binding domain of the cystic fibrosis transmembrane conductance regulator yields insight into disease-causing mutationsJournal of Biological Chemistry.  292:14147-14164. 2017
2017 Direct binding of the Corrector VX-809 to Human CFTR NBD1: Evidence of an Allosteric coupling between the Binding site and the NBD1:CL4 Interface sMolecular Pharmacology.  92:124-135. 2017
2017 Interactions and cooperativity between P-glycoprotein structural domains determined by thermal unfolding provides insights into its solution structure and function 2017
2016 A Guide to Differential Scanning Calorimetry of Membrane and Soluble Proteins in DetergentsMethods in Enzymology.  567:319-358. 2016
2015 Restoration of NBD1 thermal stability is necessary and sufficient to correct Δf508 CFTR folding and assemblyJournal of Molecular Biology.  427:106-120. 2015
2014 Membrane protein stability can be compromised by detergent interactions with the extramembranous soluble domainsProtein Science.  23:769-789. 2014
2012 Conformational changes relevant to channel activity and folding within the first nucleotide binding domain of the cystic fibrosis transmembrane conductance regulatorJournal of Biological Chemistry.  287:28480-28494. 2012
2011 A gene optimization strategy that enhances production of fully functional P-Glycoprotein in Pichia pastorisPLoS ONE.  6. 2011
2010 Integrated biophysical studies implicate partial unfolding of NBD1 of CFTR in the molecular pathogenesis of F508del cystic fibrosisProtein Science.  19:1932-1947. 2010
2010 Thermal unfolding studies show the disease causing F508del mutation in CFTR thermodynamically destabilizes nucleotide-binding domain 1Protein Science.  19:1917-1931. 2010
2010 Structures of a minimal human CFTR first nucleotide-binding domain as a monomer, head-to-tail homodimer, and pathogenic mutant 2010
2009 SAR studies for a new class of antibacterial NAD biosynthesis inhibitorsACS Combinatorial Science.  11:617-625. 2009
2009 Virtual screening to identify lead inhibitors for bacterial NAD synthetase (NADs)Bioorganic and Medicinal Chemistry Letters.  19:2001-2005. 2009
2008 Structure of nicotinic acid mononucleotide adenylyltransferase from Bacillus anthracis 2008
2007 Structural adaptation of an interacting non-native C-terminal helical extension revealed in the crystal structure of NAD+ synthetase from Bacillus anthracisActa Crystallographica Section D: Biological Crystallography.  63:891-905. 2007
2007 Antibacterial nicotinamide adenine dinucleotide synthetase inhibitors: Amide- and ether-linked tethered dimers with α-amino acid end groupsJournal of Medicinal Chemistry.  50:2612-2621. 2007
2007 His-tag impact on structureActa Crystallographica Section D: Biological Crystallography.  63:295-301. 2007
2005 Förster resonance energy transfer measurements are consistent with a helical bundle model for lipid-free apolipoprotein A-IBiochemistry.  44:16413-16425. 2005
2005 Engineering mouse apolipoprotein A-I into a monomeric, active protein useful for structural determinationBiochemistry.  44:14907-14919. 2005
2005 Tethered dimer inhibitors of NAD synthetase: Parallel synthesis of an aryl-substituted SAR libraryACS Combinatorial Science.  7:898-904. 2005
2004 Role of inhibitor aliphatic chain in the thermodynamics of inhibitor binding to Escherichia coli enoyl-ACP reductase and the Phe203Leu mutant: A proposed mechanism for drug resistanceBiochemistry.  43:13380-13389. 2004
2004 Dimethyl sulfoxide at 2.5% (v/v) alters the structural cooperativity and unfolding mechanism of dimeric bacterial NAD+ synthetaseProtein Science.  13:830-841. 2004
2003 Deletion of DnaK's lid strengthens binding to the nucleotide exchange factor, GrpE: A kinetic and thermodynamic analysisBiochemistry.  42:9028-9040. 2003
2003 Tethered dimers as NAD synthetase inhibitors with antibacterial activityJournal of Medicinal Chemistry.  46:3371-3381. 2003
2002 NH3-dependent NAD+ synthetase from Bacillus subtilis at 1 Å resolutionActa Crystallographica Section D: Biological Crystallography.  58:1138-1146. 2002
2001 Kinetic and calorimetric evidence for two distinct scaffolding protein binding populations within the bacteriophage P22 procapsidBiochemistry.  40:8962-8970. 2001
2001 Stabilization of active-site loops in NH3-dependent NAD+ synthetase from Bacillus subtilisActa Crystallographica Section D: Biological Crystallography.  57:806-812. 2001
2001 Structural models of human apolipoprotein A-I: A critical analysis and review 2001
2000 Stereoselective binding of an enantiomeric pair of stromelysin-1 inhibitors caused by conformational entropy factorsBioorganic and Medicinal Chemistry Letters.  10:2427-2430. 2000
2000 Down-regulation of cell surface receptors is modulated by polar residues within the transmembrane domain 2000
1999 Human apolipoprotein A-I: Structure determination and analysis of unusual diffraction characteristicsActa Crystallographica Section D: Biological Crystallography.  55:2013-2021. 1999
1999 Analysis of the binding of hydroxamic acid and carboxylic acid inhibitors to the stromelysin-1 (matrix metalloproteinase-3) catalytic domain by isothermal titration calorimetryBiochemistry.  38:13592-13601. 1999
1999 Crystallization of truncated human apolipoprotein A-I in a novel conformationActa Crystallographica Section D: Biological Crystallography.  55:1578-1583. 1999
1999 The structure of human lipoprotein A-I. Evidence for the 'belt' modelJournal of Biological Chemistry.  274:14541-14544. 1999
1998 The lipid-free structure of apolipoprotein A-I: Effects of amino- terminal deletionsBiochemistry.  37:11714-11725. 1998
1998 Structural analysis of apolipoprotein A-I: Effects of amino- and carboxy-terminal deletions on the lipid-free structureBiochemistry.  37:945-955. 1998
1997 Crystal structure of truncated human apolipoprotein A-I suggests a lipid-bound conformation 1997
1997 Structural analysis of apolipoprotein A-I: Limited proteolysis of methionine-reduced and -oxidized lipid-free and lipid-bound human apo A-IBiochemistry.  36:7615-7624. 1997
1997 Truncation of the amine terminus of human apolipoprotein A-I substantially alters only the lipid-free conformationBiochemistry.  36:288-300. 1997
1996 Kinetic and structural analysis of mutant CD4 receptors that are defective in HIV gp120 binding 1996
1996 Storage stability of the solution formulation of sCD4 determined by DSC in comparison with two functional assays 1996
1995 Structural models of human apolipoprotein A-IBBA - Lipids and Lipid Metabolism.  1256:103-129. 1995
1995 Interdomain Communication of T-Cell CD4 Studied by Absorbance and Fluorescence Difference Spectroscopy Measurements of Urea-Induced UnfoldingBiochemistry.  34:6464-6474. 1995
1994 The amphipathic α helix: A multifunctional structural motif in plasma apolipoproteinsAdvances in Protein Chemistry.  45:303-369. 1994
1993 Intracellular transport and virion incorporation of vpx requires interaction with other virus type-specific componentsVirology.  193:222-233. 1993
1992 Analysis of the Two-State Behavior of the Thermal Unfolding of Serum Retinol Binding Protein Containing a Single Retinol LigandBiochemistry.  31:5560-5567. 1992
1992 The amphipathic helix in the exchangeable apolipoproteins: A review of secondary structure and functionJournal of Lipid Research.  33:141-166. 1992
1992 Thermal stabilities of mutant Escherichia coli tryptophan synthase α subunitsArchives of Biochemistry and Biophysics.  292:34-41. 1992
1991 Role of amphipathic helixes in HDL structure/functionAdvances in human genetics.  285:131-140. 1991
1990 Amphipathic helix motif: Classes and properties 1990
1990 Use of synthetic peptide analogues to localize lecithin:cholesterol acyltransferase activating domain in apolipoprotein A-IArteriosclerosis (Dallas, Tex.).  10:95-105. 1990
1989 Characterization of apoA-I-containing lipoprotein subpopulations secreted by HepG2 cellsJournal of Lipid Research.  30:1429-1436. 1989
1989 Structure and thermal stability of monomeric bacteriorhodopsin in mixed pospholipid/detergent micelles 1989
1987 Characterization of high density lipoprotein subspecies: Structural studies by single vertical spin ultracentrifugation and immunoaffinity chromatographyJournal of Lipid Research.  28:913-929. 1987
1987 pH Dependence of Bacteriorhodopsin Thermal UnfoldingBiochemistry.  26:7431-7438. 1987
1985 Studies of synthetic peptide analogs of the amphiphatic helix. Correlation of structure with functionJournal of Biological Chemistry.  260:10256-10262. 1985
1985 Studies of synthetic peptide analogs of the amphiphatic helix. Structure of complexes with dimyristoyl phosphatidylcholineJournal of Biological Chemistry.  260:10248-10255. 1985
1984 Structural Studies of Apolipoprotein A-I/Phosphatidylcholine Recombinants by High-Field Proton NMR, Nondenaturing Gradient Gel Electrophoresis, and Electron MicroscopyBiochemistry.  23:359-367. 1984
1983 Studies of synthetic peptide analogs of the amphipathic helix. Competitive displacement of exchangeable apolipoproteins from native lipoproteinsJournal of Biological Chemistry.  258:2290-2295. 1983
1982 Structural domains of vesicular stomatitis virus. A study by differential scanning calorimetry, thermal gel analysis, and thermal electron microscopy.Journal of Biological Chemistry.  257:12-15. 1982
1982 Dynamics of Apolipoprotein-Phospholipid InteractionsBiophysical Journal.  37:172-173. 1982
1982 Minimal Size Phosphatidylcholine Vesicles: Effects of Radius of Curvature on Head Group Packing and ConformationBiochemistry.  21:4569-4575. 1982
1982 Structural Domains in Vesicular Stomatitis Virus Membrane as Studied by Differential Scanning CalorimetryBiophysical Journal.  37:25-26. 1982

Education And Training

  • Doctor of Philosophy in Medicinal and Pharmaceutical Chemistry, University of Kansas 1979
  • Full Name

  • Christie Brouillette