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Brouillette Ph.D., Christie
Professor Emeritus
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E-mail
christie@uab.edu
Office Address
CBSE 234
CENTER FOR BIOPHYSICAL SCIENCES AND ENGINEERING
Birmingham, AL 35294-4400
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Brouillette Ph.D., Christie
Professor Emeritus
Positions
Senior Scientist
,
Experimental Therapeutics
,
O'Neal Comprehensive Cancer Center
Senior Scientist (C)
,
O'Neal Comprehensive Cancer Center
,
School of Medicine
2014 -
Professor - Emeritus (P)
,
Biochemistry & Molecular Genetics
,
Academic Joint Departments
2018 -
Publications
Research
Background
Contact
Publications
Selected Publications
Academic Article
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Year
Title
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2018
Structural stability of purified human CFTR is systematically improved by mutations in nucleotide binding domain 1
.
BBA - Biomembranes
. 1860:1193-1204.
2018
2018
Ligand binding to a remote site thermodynamically corrects the F508del mutation in the human cystic fibrosis transmembrane conductance regulator
.
Journal of Biological Chemistry
. 293:17685-17704.
2018
2017
Stabilization of a nucleotide-binding domain of the cystic fibrosis transmembrane conductance regulator yields insight into disease-causing mutations
.
Journal of Biological Chemistry
. 292:14147-14164.
2017
2017
Direct binding of the Corrector VX-809 to Human CFTR NBD1: Evidence of an Allosteric coupling between the Binding site and the NBD1:CL4 Interface
s
.
Molecular Pharmacology
. 92:124-135.
2017
2017
Interactions and cooperativity between P-glycoprotein structural domains determined by thermal unfolding provides insights into its solution structure and function
.
BBA - Biomembranes
. 1859:48-60.
2017
2016
A Guide to Differential Scanning Calorimetry of Membrane and Soluble Proteins in Detergents
.
Methods in Enzymology
. 567:319-358.
2016
2015
Restoration of NBD1 thermal stability is necessary and sufficient to correct Δf508 CFTR folding and assembly
.
Journal of Molecular Biology
. 427:106-120.
2015
2014
Membrane protein stability can be compromised by detergent interactions with the extramembranous soluble domains
.
Protein Science
. 23:769-789.
2014
2012
Conformational changes relevant to channel activity and folding within the first nucleotide binding domain of the cystic fibrosis transmembrane conductance regulator
.
Journal of Biological Chemistry
. 287:28480-28494.
2012
2011
A gene optimization strategy that enhances production of fully functional P-Glycoprotein in Pichia pastoris
.
PLoS ONE
. 6.
2011
2010
Integrated biophysical studies implicate partial unfolding of NBD1 of CFTR in the molecular pathogenesis of F508del cystic fibrosis
.
Protein Science
. 19:1932-1947.
2010
2010
Thermal unfolding studies show the disease causing F508del mutation in CFTR thermodynamically destabilizes nucleotide-binding domain 1
.
Protein Science
. 19:1917-1931.
2010
2010
Structures of a minimal human CFTR first nucleotide-binding domain as a monomer, head-to-tail homodimer, and pathogenic mutant
.
Protein Engineering -Oxford-
. 23:375-384.
2010
2009
SAR studies for a new class of antibacterial NAD biosynthesis inhibitors
.
Journal of Combinatorial Chemistry
. 11:617-625.
2009
2009
Virtual screening to identify lead inhibitors for bacterial NAD synthetase (NADs)
.
Bioorganic and Medicinal Chemistry Letters
. 19:2001-2005.
2009
2008
Structure of nicotinic acid mononucleotide adenylyltransferase from Bacillus anthracis
.
Acta Crystallographica Section F: Structural Biology and Crystallization Communications
. 64:893-898.
2008
2007
Structural adaptation of an interacting non-native C-terminal helical extension revealed in the crystal structure of NAD
+
synthetase from Bacillus anthracis
.
Acta Crystallographica Section D: Biological Crystallography
. 63:891-905.
2007
2007
Antibacterial nicotinamide adenine dinucleotide synthetase inhibitors: Amide- and ether-linked tethered dimers with α-amino acid end groups
.
Journal of Medicinal Chemistry
. 50:2612-2621.
2007
2007
His-tag impact on structure
.
Acta Crystallographica Section D: Biological Crystallography
. 63:295-301.
2007
2005
Förster resonance energy transfer measurements are consistent with a helical bundle model for lipid-free apolipoprotein A-I
.
Biochemistry
. 44:16413-16425.
2005
2005
Engineering mouse apolipoprotein A-I into a monomeric, active protein useful for structural determination
.
Biochemistry
. 44:14907-14919.
2005
2005
Tethered dimer inhibitors of NAD synthetase: Parallel synthesis of an aryl-substituted SAR library
.
Journal of Combinatorial Chemistry
. 7:898-904.
2005
2004
Role of inhibitor aliphatic chain in the thermodynamics of inhibitor binding to Escherichia coli enoyl-ACP reductase and the Phe203Leu mutant: A proposed mechanism for drug resistance
.
Biochemistry
. 43:13380-13389.
2004
2004
Dimethyl sulfoxide at 2.5% (v/v) alters the structural cooperativity and unfolding mechanism of dimeric bacterial NAD
+
synthetase
.
Protein Science
. 13:830-841.
2004
2003
Deletion of DnaK's lid strengthens binding to the nucleotide exchange factor, GrpE: A kinetic and thermodynamic analysis
.
Biochemistry
. 42:9028-9040.
2003
2003
Tethered dimers as NAD synthetase inhibitors with antibacterial activity
.
Journal of Medicinal Chemistry
. 46:3371-3381.
2003
2002
NH
3
-dependent NAD
+
synthetase from Bacillus subtilis at 1 Å resolution
.
Acta Crystallographica Section D: Biological Crystallography
. 58:1138-1146.
2002
2001
Kinetic and calorimetric evidence for two distinct scaffolding protein binding populations within the bacteriophage P22 procapsid
.
Biochemistry
. 40:8962-8970.
2001
2001
Stabilization of active-site loops in NH
3
-dependent NAD
+
synthetase from Bacillus subtilis
.
Acta Crystallographica Section D: Biological Crystallography
. 57:806-812.
2001
2001
Structural models of human apolipoprotein A-I: A critical analysis and review
.
Biochimica et Biophysica Acta Molecular and Cell Biology of Lipids
. 1531:4-46.
2001
2000
Stereoselective binding of an enantiomeric pair of stromelysin-1 inhibitors caused by conformational entropy factors
.
Bioorganic and Medicinal Chemistry Letters
. 10:2427-2430.
2000
2000
Down-regulation of cell surface receptors is modulated by polar residues within the transmembrane domain
.
Cell regulation
. 11:2643-2655.
2000
1999
Human apolipoprotein A-I: Structure determination and analysis of unusual diffraction characteristics
.
Acta Crystallographica Section D: Biological Crystallography
. 55:2013-2021.
1999
1999
Analysis of the binding of hydroxamic acid and carboxylic acid inhibitors to the stromelysin-1 (matrix metalloproteinase-3) catalytic domain by isothermal titration calorimetry
.
Biochemistry
. 38:13592-13601.
1999
1999
Crystallization of truncated human apolipoprotein A-I in a novel conformation
.
Acta Crystallographica Section D: Biological Crystallography
. 55:1578-1583.
1999
1999
The structure of human lipoprotein A-I. Evidence for the 'belt' model
.
Journal of Biological Chemistry
. 274:14541-14544.
1999
1998
The lipid-free structure of apolipoprotein A-I: Effects of amino- terminal deletions
.
Biochemistry
. 37:11714-11725.
1998
1998
Structural analysis of apolipoprotein A-I: Effects of amino- and carboxy-terminal deletions on the lipid-free structure
.
Biochemistry
. 37:945-955.
1998
1997
Crystal structure of truncated human apolipoprotein A-I suggests a lipid-bound conformation
.
Proceedings of the National Academy of Sciences
. 94:12291-12296.
1997
1997
Structural analysis of apolipoprotein A-I: Limited proteolysis of methionine-reduced and -oxidized lipid-free and lipid-bound human apo A-I
.
Biochemistry
. 36:7615-7624.
1997
1997
Truncation of the amine terminus of human apolipoprotein A-I substantially alters only the lipid-free conformation
.
Biochemistry
. 36:288-300.
1997
1996
Kinetic and structural analysis of mutant CD4 receptors that are defective in HIV gp120 binding
.
Proceedings of the National Academy of Sciences
. 93:15030-15035.
1996
1996
Storage stability of the solution formulation of sCD4 determined by DSC in comparison with two functional assays
.
Journal of Thermal Analysis and Calorimetry
. 47:1597-1609.
1996
1995
Structural models of human apolipoprotein A-I
.
BBA - Lipids and Lipid Metabolism
. 1256:103-129.
1995
1995
Interdomain Communication of T-Cell CD4 Studied by Absorbance and Fluorescence Difference Spectroscopy Measurements of Urea-Induced Unfolding
.
Biochemistry
. 34:6464-6474.
1995
1994
The amphipathic α helix: A multifunctional structural motif in plasma apolipoproteins
.
Advances in Protein Chemistry
. 45:303-369.
1994
1993
Intracellular transport and virion incorporation of vpx requires interaction with other virus type-specific components
.
Virology
. 193:222-233.
1993
1992
Analysis of the Two-State Behavior of the Thermal Unfolding of Serum Retinol Binding Protein Containing a Single Retinol Ligand
.
Biochemistry
. 31:5560-5567.
1992
1992
The amphipathic helix in the exchangeable apolipoproteins: A review of secondary structure and function
.
Journal of Lipid Research
. 33:141-166.
1992
1992
Thermal stabilities of mutant Escherichia coli tryptophan synthase α subunits
.
Archives of Biochemistry and Biophysics
. 292:34-41.
1992
1991
Role of amphipathic helixes in HDL structure/function
.
Advances in human genetics
. 285:131-140.
1991
1990
Amphipathic helix motif: Classes and properties
.
Proteins
. 8:103-117.
1990
1990
Use of synthetic peptide analogues to localize lecithin:cholesterol acyltransferase activating domain in apolipoprotein A-I
.
Arteriosclerosis (Dallas, Tex.)
. 10:95-105.
1990
1989
Characterization of apoA-I-containing lipoprotein subpopulations secreted by HepG2 cells
.
Journal of Lipid Research
. 30:1429-1436.
1989
1989
Structure and thermal stability of monomeric bacteriorhodopsin in mixed pospholipid/detergent micelles
.
Proteins
. 5:38-46.
1989
1987
Characterization of high density lipoprotein subspecies: Structural studies by single vertical spin ultracentrifugation and immunoaffinity chromatography
.
Journal of Lipid Research
. 28:913-929.
1987
1987
pH Dependence of Bacteriorhodopsin Thermal Unfolding
.
Biochemistry
. 26:7431-7438.
1987
1985
Studies of synthetic peptide analogs of the amphiphatic helix. Correlation of structure with function
.
Journal of Biological Chemistry
. 260:10256-10262.
1985
1985
Studies of synthetic peptide analogs of the amphiphatic helix. Structure of complexes with dimyristoyl phosphatidylcholine
.
Journal of Biological Chemistry
. 260:10248-10255.
1985
1984
Structural Studies of Apolipoprotein A-I/Phosphatidylcholine Recombinants by High-Field Proton NMR, Nondenaturing Gradient Gel Electrophoresis, and Electron Microscopy
.
Biochemistry
. 23:359-367.
1984
1983
Studies of synthetic peptide analogs of the amphipathic helix. Competitive displacement of exchangeable apolipoproteins from native lipoproteins
.
Journal of Biological Chemistry
. 258:2290-2295.
1983
1982
Structural domains of vesicular stomatitis virus. A study by differential scanning calorimetry, thermal gel analysis, and thermal electron microscopy.
.
Journal of Biological Chemistry
. 257:12-15.
1982
1982
Dynamics of Apolipoprotein-Phospholipid Interactions
.
Biophysical Journal
. 37:172-173.
1982
1982
Minimal Size Phosphatidylcholine Vesicles: Effects of Radius of Curvature on Head Group Packing and Conformation
.
Biochemistry
. 21:4569-4575.
1982
1982
Structural Domains in Vesicular Stomatitis Virus Membrane as Studied by Differential Scanning Calorimetry
.
Biophysical Journal
. 37:25-26.
1982
Research
Principal Investigator On
Expression and Purification of CFTR Domain Constructs
awarded by
Cystic Fibrosis Foundation
2008 - 2018
CFTR Structural Energetics: Domain Communication, Ligand Binding Response
awarded by
Cystic Fibrosis Foundation
2016 - 2017
CFTR Structural Energetics: Domain Communication, Ligand Binding Response
awarded by
Cystic Fibrosis Foundation
2013 - 2016
Recombinant CFTR: Cooperativity and Structural Domains
awarded by
Cystic Fibrosis Foundation
2007 - 2013
Discovery and Preclinical Development of Drugs for Anthrax, Plague and Tularemia
awarded by
National Institute of Allergy and Infectious Diseases/NIH/DHHS
2006 - 2013
MicroCal Auto-iTC200; Automated High Sensitivity Isothermal Titration Calorimetry for Multiple Uses
awarded by
National Center for Research Resources/NIH/DHHS
2009 - 2010
Investigator On
A Comprehensive Evaluation of Microgravity Protein Crystallization
awarded by
NASA - National Aeronautics & Space Administration
2011 - 2016
Comprehensive Cancer Center Core Support Grant
awarded by
National Cancer Institute/NIH/DHHS
2011 - 2016
Production & Crystallization of Membrane Protein for 3D Structure
awarded by
National Institute of General Medical Sciences/NIH/DHHS
2010 - 2015
Background
Education And Training
Doctor of Philosophy in Medicinal and Pharmaceutical Chemistry,
University of Kansas
1979
Contact
Full Name
Christie
Brouillette