Positions

Overview

  • Dr. Bradley K. Yoder, Professor, completed his undergraduate studies in biochemistry and molecular biology at the University of Maryland Baltimore County (B.S. 1988) and received a Ph.D. in molecular and cellular biology from the University of Maryland in 1993. He completed his postdoctoral studies at Oak Ridge National Laboratory under the guidance of Dr. Rick Woychik, where Dr. Yoder was an Alexander Hollaender Distinguished Postdoctoral Fellow. His research over the past two decades has focused on the cellular and molecular mechanisms regulating assembly, maintenance, and function of the primary cilium utilizing complementary approaches in mice, C. elegans, and in cell culture models. Work from his laboratory has used genetic screens in C. elegans to identify proteins required for ciliogenesis and cilia mediated signaling activities and how these genes function in pathways (e.g., Daf-2 Insulin/IGF-like pathway) that regulate life span and energy homeostasis. His group has analyzed in mammalian systems how the cilium regulates critical developmental pathways and how the loss of the cilium causes abnormalities in left-right body axis specification, limb and tooth patterning, skin, and hair follicle morphogenesis, and impairs endochondral bone formation. His group is also providing critical fundamental insights into the connection between ciliary dysfunction and cystic kidney disorders, and novel roles for neuronal cilia in the regulation of satiation responses, disruption of which causes obesity and type II diabetes.
  • Selected Publications

    Academic Article

    Year Title Altmetric
    A novel dynein light intermediate chain colocalizes with the retrograde motor for intraflagellar transport at sites of axoneme assembly in Chlamydomonas and mammalian cells
    BBSome component BBS5 is required for cone photoreceptor protein trafficking and outer segment maintenanceInvestigative Ophthalmology and Visual Science.  61.
    Characterization of growth factor responsiveness and alterations in growth factor homeostasis involved in the tumorigenic conversion of mouse oval cellsGrowth Factors.  15:81-94.
    Cilia proteins control cerebellar morphogenesis by promoting expansion of the granule progenitor pool
    Complement C3 activation in cyst fluid and urine from autosomal dominant polycystic kidney disease patientsJournal of Internal Medicine.  276:539-540.
    Cystic kidney diseases: All roads lead to the cilium
    Cystin, a novel cilia-associated protein, is disrupted in the cpk mouse model of polycystic kidney disease.Journal of Clinical Investigation.  109:533-540.
    Directional cell migration and chemotaxis in wound healing response to PDGF-AA are coordinated by the primary cilium in fibroblastsCellular Physiology and Biochemistry.  279-292.
    Dysfunctional cilia lead to altered ependyma and choroid plexus function, and result in the formation of hydrocephalus
    Ectopic Phosphorylated Creb Marks Dedifferentiated Proximal Tubules in Cystic Kidney Disease
    Epidermal growth factor receptor activity mediates renal cyst formation in polycystic kidney diseaseJournal of Clinical Investigation.  101:935-939.
    Functional redundancy of the B9 proteins and nephrocystins in Caenorhabditis elegans ciliogenesis
    Gene therapy rescues cilia defects and restores olfactory function in a mammalian ciliopathy modelNature Medicine.  18:1423-1428.
    Generating conditional mutants to analyze ciliary functions: the use of Cre-lox technology to disrupt cilia in specific organs.Methods in Cell Biology.  93.
    Heterozygous Pkhd1C642* mice develop cystic liver disease and proximal tubule ectasia that mimics radiographic signs of medullary sponge kidney
    Identification of a new spore coat protein gene in the cellular slime mold Dictyostelium discoideumDevelopmental Biology.  163:49-65.
    In vivo fate mapping and expression analysis reveals molecular hallmarks of prospectively isolated adult neural stem cellsCell Stem Cell.  7:744-758.
    Increased Na+/H+ exchanger activity on the apical surface of a cilium-deficient cortical collecting duct principal cell model of polycystic kidney disease
    Interferon Regulatory Factor-5 in Resident Macrophage Promotes Polycystic Kidney Disease.
    Loss of apical monocilia on collecting duct principal cells impairs ATP secretion across the apical cell surface and ATP-dependent and flow-induced calcium signalsPurinergic Signalling.  4:155-170.
    Loss of primary cilia results in deregulated and unabated apical calcium entry in ARPKD collecting duct cells
    Loss of primary cilia upregulates renal hypertrophic signaling and promotes cystogenesisJournal of the American Society of Nephrology.  22:839-848.
    MKS and NPHP modules cooperate to establish basal body/transition zone membrane associations and ciliary gate function during ciliogenesis
    Microtubule modifications and stability are altered by cilia perturbation and in cystic kidney diseaseCytoskeleton.  70:24-31.
    Mks6 mutations reveal tissue-and cell type-specific roles for the cilia transition zoneThe FASEB Journal.  33:1440-1455.
    Non-essential role for cilia in coordinating precise alignment of lens fibres
    Normal ciliogenesis requires synergy between the cystic kidney disease genes MKS-3 and NPHP-4Journal of the American Society of Nephrology.  21:782-793.
    Polaris, a protein disrupted in orpk mutant mice, is required for assembly of renal cilium
    Primary cilia enhance kisspeptin receptor signaling on gonadotropin- releasing hormone neurons
    Proximal tubule proliferation is insufficient to induce rapid cyst formation after cilia disruptionJournal of the American Society of Nephrology.  24:456.
    Resident macrophages reprogram toward a developmental state after acute kidney injuryJCI insight.  4.
    Role of epidermal primary cilia in the homeostasis of skin and hair follicles
    Role of primary cilia in the pathogenesis of polycystic kidney diseaseJournal of the American Society of Nephrology.  18:1381-1388.
    Sensory ciliogenesis in Caenorhabditis elegans: Assignment of IFT components into distinct modules based on transport and phenotypic profiles
    Single-cell RNA sequencing identifies candidate renal resident macrophage gene expression signatures across speciesJournal of the American Society of Nephrology.  30:767-781.
    Snap Shot: Sensing and signaling by ciliaCell.  161:692-692.e1.
    Soluble levels of cytosolic tubulin regulate ciliary length control
    The Oak Ridge Polycystic Kidney mouse: Modeling ciliopathies of mice and menDevelopmental Dynamics.  237:1960-1971.
    The polycystic kidney disease proteins, polycystin-1, polycystin-2, polaris, and cystin, are co-localized in renal ciliaJournal of the American Society of Nephrology.  2508-2516.
    The zebrafish foxj1a transcription factor regulates cilia function in response to injury and epithelial stretch

    Chapter

    Year Title Altmetric
    2019 Intravital visualization of the primary cilium, tubule flow, and innate immune cells in the kidney utilizing an abdominal window imaging approach.  67-83. 2019
    2018 Cilia and polycystic kidney disease.  87-110. 2018
    2017 Inflammation and fibrosis in polycystic kidney disease.  323-344. 2017
    2013 Renal Cilia Structure, Function, and Physiology.  319-346. 2013
    2013 Neuronal cilia and obesity.  165-191. 2013

    Research Overview

  • Cilia come in both motile and immotile forms. While motile cilia were known to have essential roles in the lung and respiratory system, primary cilia were widely considered vestigial structures. A significant paradigm shift in the field occurred with the generation of mouse mutants (such as the orpk mouse) that disrupt cilia formation. These new mouse mutants revealed important and novel roles for motile and immotile cilia and demonstrated that they are essential for mammalian development and tissue function. Defects in cilia have been implicated as the cause of a large and rapidly expanding group of human syndromes (Ciliopathies) with a wide range of developmental and disease phenotypes.

    The objectives of my research program are to uncover mechanisms regulating assembly, maintenance, and functions of both motile and primary forms of cilia and to determine how defects in these processes contribute to developmental abnormalities and disease pathogenesis. To accomplish these goals, my group utilizes complementary cell, genetic, and biochemical approaches in mice, C. elegans, and cell culture to identify new proteins involved in ciliogenesis and cilia mediated signaling. Work from my group has identified novel components of the ciliary transition zone, an important domain controlling what protein enters or is retained in the cilium. We have provided critical insights into how the cilium regulates developmental pathways, such as hedgehog, and how alterations in cilia-mediated regulation of this pathway cause polydactyly, defects in endochondral bone formation, and abnormal skin and hair follicle morphogenesis. My group made fundamental contributions that connected ciliary dysfunction to the creation of cysts in the kidney, liver, and pancreas, and uncovered a new role for cilia on hypothalamic neurons in regulating satiation responses. We have shown that disruption of cilia on these neurons causes obesity and type II diabetes. We also identified genes important in regulating ciliary motility and waveform and determined that their loss in mice leads to hydrocephalus, bronchiectasis, and randomization of the left-right body axis. Importantly, as part of this work, we discovered that a mutation of one of these genes we identified in our mouse model is responsible for a form of primary ciliary dyskinesia (PCD) in humans. As in the mouse model, these human PCD patients frequently have left-right body situs defects.

    In summary, the research conducted by my group is providing essential and innovative insights into how cilia are constructed and how they establish themselves as a unique signaling and sensory organelle with a distinct protein composition the rest of the cell membrane. We have uncovered many diverse and unexpected roles for cilia during development and in maintaining mammalian health.
  • Principal Investigator On

  • UAB Pilot Center for Precision Animal Modeling (C-PAM)  awarded by NIH - OFFICE OF THE DIRECTOR 2020 - 2025
  • UAB Pilot Center for Precision Animal Modeling (C-PAM) - Coordination Section  awarded by NIH - OFFICE OF THE DIRECTOR 2020 - 2025
  • UAB Childhood Cystic Kidney Disease Core Center (UAB-CCKDCC)  awarded by National Institute of Diabetes and Digestive and Kidney Diseases/NIH/DHHS 2020 - 2025
  • UAB Childhood Cystic Kidney Disease Core Center (UAB-CCKDCC) - Administrative Core  awarded by National Institute of Diabetes and Digestive and Kidney Diseases/NIH/DHHS 2020 - 2025
  • UAB Childhood Cystic Kidney Disease Core Center (UAB-CCKDCC) - Core A - Clinical, Translational, and Biorepository Resource  awarded by National Institute of Diabetes and Digestive and Kidney Diseases/NIH/DHHS 2020 - 2025
  • UAB Childhood Cystic Kidney Disease Core Center (UAB-CCKDCC) - Core C - In Vivo Bioassay and Model Development Resource  awarded by National Institute of Diabetes and Digestive and Kidney Diseases/NIH/DHHS 2020 - 2025
  • Intravital Analysis of Cilia Function during Injury in the Kidney  awarded by National Institute of Diabetes and Digestive and Kidney Diseases/NIH/DHHS 2020 - 2023
  • Training Program in Cell, Molecular, and Developmental Biology  awarded by National Institute of General Medical Sciences/NIH/DHHS 2018 - 2023
  • Nphp4 and Bbs5 are Required for Ciliary Mediated Cell Signaling Events that Regulate Cardiac Development in the Mouse  awarded by National Heart, Lung, and Blood Institute/NIH/DHHS 2020 - 2023
  • Injury Response Mediated Pathogenesis in Renal Ciliopathies  awarded by National Institute of Diabetes and Digestive and Kidney Diseases/NIH/DHHS 2018 - 2021
  • Understanding Ciliary Functions in Mammalian Development  awarded by University of California, San Francisco 2016 - 2021
  • Application of Progenitor Niche Signals to Ex Vivo Nephrogenesis  awarded by University of Texas Southwestern Medical Center at Dallas 2020 - 2021
  • The Primary Cilium as A Novel Regulator of The Immune Response in The Kidney  awarded by National Institute of Diabetes and Digestive and Kidney Diseases/NIH/DHHS 2019 - 2021
  • Acquiring A Recirculating Aquaria System With Automated-Feed For Zebrafish  awarded by UNIVERSITY OF ALABAMA HEALTH SERVICES FOUNDATION 2018 - 2020
  • Hepato/Renal Fibrocystic Diseases Core Center (UAB HRFDCC) - Admin Core  awarded by National Institute of Diabetes and Digestive and Kidney Diseases/NIH/DHHS 2015 - 2020
  • Hepato/Renal Fibrocystic Diseases Core Center (UAB HRFDCC) - Hepato/Renal Fibrocystic Kidney Disease Core Center - Summer Research Internship  awarded by National Institute of Diabetes and Digestive and Kidney Diseases/NIH/DHHS 2015 - 2020
  • Hepato/Renal Fibrocystic Diseases Core Center (UAB HRFDCC) - Pilot and Feasibility Program  awarded by National Institute of Diabetes and Digestive and Kidney Diseases/NIH/DHHS 2015 - 2020
  • Hepato/Renal Fibrocystic Diseases Core Center (UAB HRFDCC) - The Hepato/Renal Fibrocystic Diseases Cellular Physiology Resource - Core C  awarded by National Institute of Diabetes and Digestive and Kidney Diseases/NIH/DHHS 2015 - 2020
  • Hepato/Renal Fibrocystic Diseases Core Center (UAB HRFDCC) - The Hepato/Renal Fibrocystic Diseases Engineered Models Resource - Core B  awarded by National Institute of Diabetes and Digestive and Kidney Diseases/NIH/DHHS 2015 - 2020
  • Hepato/Renal Fibrocystic Diseases Core Center (UAB HRFDCC) - The Hepato/Renal Fibrocystic Diseases Translational Resource - Core A  awarded by National Institute of Diabetes and Digestive and Kidney Diseases/NIH/DHHS 2015 - 2020
  • The Hepato/Renal Fibrocystic Disease Core Center (UAB HRFDCC)  awarded by National Institute of Diabetes and Digestive and Kidney Diseases/NIH/DHHS 2015 - 2020
  • Epigenetic Regulation of Kir4.1 and GLT1 in Pathophysiology  awarded by VIRGINIA TECH UNIVERSITY 2017 - 2019
  • Cilia and Cystic Kidney Disease  awarded by National Institute of Diabetes and Digestive and Kidney Diseases/NIH/DHHS 2013 - 2019
  • Predoctoral Training in Cell and Molecular Biology  awarded by National Institute of General Medical Sciences/NIH/DHHS 2013 - 2018
  • Ciliogenesis in Epithelial Injury  awarded by MASSACHUSETTS GENERAL HOSPITAL 2014 - 2018
  • Injury Response Mediated Pathogenesis in Ciliopathies  awarded by Polycystic Kidney Disease Foundation 2016 - 2018
  • Hepato/Renal Fibrocystic Diseases Core Center (UAB HRFDCC)  awarded by National Institute of Diabetes and Digestive and Kidney Diseases/NIH/DHHS 2011 - 2016
  • Hepato/Renal Fibrocystic Diseases Core Center (UAB HRFDCC) - Administrative Core  awarded by National Institute of Diabetes and Digestive and Kidney Diseases/NIH/DHHS 2011 - 2016
  • Hepato/Renal Fibrocystic Diseases Core Center (UAB HRFDCC) - Core A  awarded by National Institute of Diabetes and Digestive and Kidney Diseases/NIH/DHHS 2011 - 2016
  • Hepato/Renal Fibrocystic Diseases Core Center (UAB HRFDCC) - Core B  awarded by National Institute of Diabetes and Digestive and Kidney Diseases/NIH/DHHS 2011 - 2016
  • Hepato/Renal Fibrocystic Diseases Core Center (UAB HRFDCC) - Core C MUSC  awarded by National Institute of Diabetes and Digestive and Kidney Diseases/NIH/DHHS 2011 - 2016
  • Hepato/Renal Fibrocystic Diseases Core Center (UAB HRFDCC) - Summer Research Internship  awarded by National Institute of Diabetes and Digestive and Kidney Diseases/NIH/DHHS 2011 - 2016
  • Private Grant  awarded by OTSUKA AMERICA PHARMACEUTICAL, INC. 2016
  • Private Grant  awarded by RELYPSA - NEW 2016
  • In Vivo Analysis of Cilia Mechanosensation in the Kidney  awarded by PKD Foundation for Research in Polycystic Kidney Disease 2014 - 2016
  • Intraflagellar Transport Mediated Regulation of Hedgehog Signaling  awarded by National Institute of Child Health and Human Development/NIH/DHHS 2008 - 2014
  • Role of Cilia/IFT in Skin and Hair  awarded by National Institute of Arthritis & Musculoskeletal & Skin Diseases/NIH/DHHS 2008 - 2014
  • Predoctoral Training in Cellular and Molecular Biology  awarded by National Institute of General Medical Sciences/NIH/DHHS 2009 - 2013
  • Private Grant  awarded by Genentech 2012 - 2013
  • Cilia and Cystic Kidney Disease  awarded by National Institute of Diabetes and Digestive and Kidney Diseases/NIH/DHHS 2008 - 2013
  • Cilial Dysfunction and Pathogenesis of Obesity  awarded by National Institute of Diabetes and Digestive and Kidney Diseases/NIH/DHHS 2006 - 2012
  • The Role of Primary Neuronal Cilia in Appetite and Satiation  awarded by National Institute of Diabetes and Digestive and Kidney Diseases/NIH/DHHS 2010 - 2012
  • Hepato/Renal Fibrocystic Diseases Core Center (UAB HRFDCC) - Core B  awarded by National Institute of Diabetes and Digestive and Kidney Diseases/NIH/DHHS 2010 - 2011
  • UAB Recessive PKD Research and Translational Core Center - Core B  awarded by National Institute of Diabetes and Digestive and Kidney Diseases/NIH/DHHS 2005 - 2010
  • Rheumatic Diseases Core Center: Pilot Project - P & F 1  awarded by National Institute of Arthritis & Musculoskeletal & Skin Diseases/NIH/DHHS 2008 - 2010
  • Investigator On

  • UAB Pilot Center for Precision Animal Modeling (C-PAM)  awarded by NIH - OFFICE OF THE DIRECTOR 2020 - 2025
  • UAB Pilot Center for Precision Animal Modeling (C-PAM) - Disease Modeling Unit  awarded by NIH - OFFICE OF THE DIRECTOR 2020 - 2025
  • Interdisciplinary Training In Kidney-Related Research  awarded by National Institute of Diabetes and Digestive and Kidney Diseases/NIH/DHHS 2019 - 2024
  • PRedOctoral Phd and MD Research Training In TEams (PROmoTE)  awarded by National Institute of Diabetes and Digestive and Kidney Diseases/NIH/DHHS 2019 - 2024
  • Mononuclear Phagocytes in the Pathogenesis of Acute Kidney Injury  awarded by National Institute of Diabetes and Digestive and Kidney Diseases/NIH/DHHS 2019 - 2023
  • Kidney Undergraduate Research Experience (KURE)  awarded by National Institute of Diabetes and Digestive and Kidney Diseases/NIH/DHHS 2018 - 2023
  • PRedoctoral Interdisciplinary Training in Renal Physiology and MEdIcIne (PRIME)  awarded by National Institute of Diabetes and Digestive and Kidney Diseases/NIH/DHHS 2018 - 2023
  • The Role of Ldb1 in Regulating Pancreatic Islet Cell Delamination and Differentiation  awarded by National Institute of Diabetes and Digestive and Kidney Diseases/NIH/DHHS 2019 - 2022
  • The Role of Lhx1 in Pancreatic Beta Cell Development and Function  awarded by National Institute of Diabetes and Digestive and Kidney Diseases/NIH/DHHS 2017 - 2021
  • Improving Resolution and Throughput on the HRIF Institutional Core  awarded by UNIVERSITY OF ALABAMA HEALTH SERVICES FOUNDATION 2018 - 2020
  • Hepato/Renal Fibrocystic Diseases Core Center (UAB HRFDCC) - The Hepato/Renal Fibrocystic Diseases Therapeutic and Screening Resource - Core D  awarded by National Institute of Diabetes and Digestive and Kidney Diseases/NIH/DHHS 2015 - 2020
  • The Hepato/Renal Fibrocystic Disease Core Center (UAB HRFDCC)  awarded by National Institute of Diabetes and Digestive and Kidney Diseases/NIH/DHHS 2015 - 2020
  • Genetic Regulation of Unconventional Prostaglandin Metobolism  awarded by National Institute of General Medical Sciences/NIH/DHHS 2018 - 2020
  • Interdisciplinary Training in Kidney-Related Research  awarded by National Institute of Diabetes and Digestive and Kidney Diseases/NIH/DHHS 2014 - 2019
  • Mechanisms of C3 Effects in ARPKD Pathogenesis  awarded by National Institute of Diabetes and Digestive and Kidney Diseases/NIH/DHHS 2013 - 2018
  • UAB Obesity Training Program  awarded by National Institute of Diabetes and Digestive and Kidney Diseases/NIH/DHHS 2014 - 2017
  • Light-Induced Genetic Alterations Within Single Cell of a Live Vertebrate Animal  awarded by National Institute of Neurological Disorders and Stroke/NIH/DHHS 2014 - 2016
  • Hepato/Renal Fibrocystic Diseases Core Center (UAB HRFDCC)  awarded by National Institute of Diabetes and Digestive and Kidney Diseases/NIH/DHHS 2011 - 2016
  • Human Heme Oxygenase-1 Gene Regulation in Renal Injury  awarded by National Institute of Diabetes and Digestive and Kidney Diseases/NIH/DHHS 2008 - 2014
  • Rheumatic Diseases Core Center  awarded by National Institute of Arthritis & Musculoskeletal & Skin Diseases/NIH/DHHS 2008 - 2012
  • Hepato/Renal Fibrocystic Diseases Core Center (UAB HRFDCC)  awarded by National Institute of Diabetes and Digestive and Kidney Diseases/NIH/DHHS 2010 - 2011
  • UAB Recessive PKD Research and Translational Core Center  awarded by National Institute of Diabetes and Digestive and Kidney Diseases/NIH/DHHS 2005 - 2010
  • Teaching Activities

  • GBSC700 - Journal Clubs (Spring Term 2020) 2020
  • GBSC700 - Journal Clubs (Fall Term 2019) 2019
  • GBS712 - Cell & Mol Aspects Dev Bio (Spring Term 2019) 2019
  • GBSC700 - Journal Clubs (Spring Term 2019) 2019
  • GBSC733 - Art of Reproducible Science (Spring Term 2019) 2019
  • GBS712 - Cell/Mol Aspects Dev Bio (Spring Term 2017) 2017
  • GBS746 - Special Topics (Fall Term 2016) 2016
  • GBS792 - CMDB Seminar (Spring Term 2016) 2016
  • GBS792 - CMDB Seminar (Fall Term 2015) 2015
  • GBS712 - Cell/Mol Aspects Dev Bio (Spring Term 2015) 2015
  • GBS792 - CMDB Seminar (Spring Term 2015) 2015
  • GBS792 - CMDB Seminar (Fall Term 2014) 2014
  • GBS712 - Cell/Mol Aspects Dev Bio (Spring Term 2014) 2014
  • GBS787 - Special Topics (Spring Term 2014) 2014
  • GBS792 - CMDB Seminar (Spring Term 2014) 2014
  • GBS792 - CMDB Seminar (Fall Term 2013) 2013
  • GBS712 - Cell/Mol Aspects Dev Bio (Spring Term 2013) 2013
  • GBS792 - CMDB Seminar (Spring Term 2013) 2013
  • GBS792 - CMDB Seminar (Fall Term 2012) 2012
  • GBS712 - Cell/Mol Aspects Dev Bio (Spring Term 2012) 2012
  • GBS792 - CMDB Seminar (Spring Term 2012) 2012
  • GBS712 - CELL AND MOLECULAR ASPECTS OF DEVELOPMENTAL BIOLOGY (Spring Term 2012) 2012
  • GBS792 - CMDB Seminar (Fall Term 2011) 2011
  • GBS712 - Cell/Mol Aspects Dev Bio (Spring Term 2011) 2011
  • CB747 - Cell Biology Seminar (Spring Term 2010) 2010
  • CMB755 - CMB V Cell & Mole Aspt Dev BY (Spring Term 2010) 2010
  • CB747 - Cell Biology Seminar (Fall Term 2009) 2009
  • CMB755 - CMB V Cell & Mole Aspt Dev BY (Spring Term 2009) 2009
  • CB747 - Cell Biology Seminar (Fall Term 2008) 2008
  • CB747 - Cell Biology Seminar (Spring Term 2008) 2008
  • CMB755 - CMB V Cell & Mole Aspt Dev BY (Spring Term 2008) 2008
  • CB747 - Cell Biology Seminar (Fall Term 2007) 2007
  • Full Name

  • Bradley Yoder