Positions

Research Overview

  • Our laboratory is focused on understanding the molecular features of epithelial cancers such as prostate, pancreas, mammary, colorectal and ovarian adenocarcinomas as well as squamous cell lesions of oral cavity, esophagus, lung, cervix and skin in order to identify biomarkers associated either with early pre-invasive neoplastic lesions or with advanced stage malignant lesions. Of special interest are biomarkers that can be used to aid in determining prognosis or risk assessment or in predicting therapy. For example, the earliest putative pre-invasive lesion of the prostate is prostatic intraepithelial neoplasia (PIN); we have demonstrated the phenotypic expression of molecular markers in PIN is similar to the phenotypic expression of these same molecular markers in prostatic adenocarcinomas. We study the escape of PCa from the requirement for androgens, i.e., development of androgen resistance. We use the human xenograft tumor models CWR22, LNCaP, DU145 and PC-3 in nude mice to investigate androgen effects on prostate tumors and the development of androgen resistance in these tumors. These data are supplemented by in vitro studies. In colorectal adenocarcinoma (CRCs), we have identified six molecular markers that aid in identifying aggressive subgroups of CRCs. These are p53, Bcl-2, p27kip-1, Suppressin, MUC-1 and MUC-2. We have identified differences in prognostic usefulness of biomarkers based on the anatomic location of CRCs. Our studies also have focused on the racial differences in the expression of these molecular markers as well as racial differences in the clinical importance of the expression of these biomarkers. Our studies of breast neoplasia also have emphasized racial differences in molecular features of ductal carcinoma. Besides our interests in epithelial neoplasia, we have a broad interest in diffuse pulmonary diseases including pulmonary arterial hypertension as well as tissue reparative processes leading to granulation tissues and neovascularity. In general our laboratory utilizes proteomic techniques but also has capabilities in molecular biology. In addition to quantitative immunohistochemistry (fluorescent or bright field), Western blotting, high throughput ELISA, multiplex immunoassays (Luminex and MESO), we have a SELDI-TOF-MS system for identification of unique proteomic patterns and we collaborate using other mass spectrometry systems in the early detection of cancers by determining the proteins of specific cancers in biological fluids such as serum. We also have automated cytomorphometric instrumentation and tissue arrays that can be used in multi-tissue analyses. Similarly, we can use gene chip (affymetrix) and spotted array analysis in gene discovery. Of great interest are collaborative efforts to improve the biomathematic/statistical approaches to evaluating biomarkers and multiplex methods of analysis and how bias in collecting and processing tissue samples may affect multiplex assays. Also, for the last two decades we have provided human tissues to support the research of biomedical investigators throughout North America. In this effort, sponsored primarily by the Cooperative Human Tissue Network (CHTN), we have introduced to tissue resources the utilization of quality control of tissues provided for research and we have been involved in database development for tissue resources. We also have great expertise in how fixation of tissues interacts with the stages of tissue processing to affect immunorecognition and other molecular assays.

    Grant Support (P.I.)

    Biomarker Reference Laboratory – Early Detection Research Network (EDRN)
    Tissue Resource and Molecular Pathology Core of the Breast SPORE
    Tissue Research and Molecular Pathology Core of the Pancreatic SPORE
    Cooperative Human Tissue Network (CHTN)
    Pulmonary Hypertension Breakthrough Initiative (PHBI)
    EDRN

    Goals – Multiplex Assays in Early Detection, Risk Assessment and Prognosis.
  • Principal Investigator On

  • A Metabolomics Approach to Define a Novel Chemopreventive Pathway  awarded by University of South Alabama
  • Active - Tissue Procurement - Comprehensive Cancer Center Core Support Grant  awarded by National Cancer Institute/NIH/DHHS
  • Biomarkers in the Detection of Prostate Cancer in African Americans  awarded by DOD - ARMY MEDICAL RESEARCH ACQUISITION ACTIVITY
  • COMPLETED - Morehouse School of Medicine/Tuskegee University/University of Alabama Cancer Center Partnership: Project 2: Molecular Regulation of Kaiso in Prostate Cancer  awarded by National Cancer Institute/NIH/DHHS
  • Center for Tissue Processing to Support Research in Pulmonary Arterial Hypertension  awarded by CARDIOVASCULAR MEDICAL RESEARCH AND EDUCATION FUND
  • Collaborative Human Tissue Network  awarded by National Cancer Institute/NIH/DHHS
  • Comprehensive Cancer Center Core Support Grant - Tissue Procurement  awarded by National Cancer Institute/NIH/DHHS
  • Comprehensive Cancer Center Core Support Grant - Tissue Procurement Shared Facility  awarded by National Cancer Institute/NIH/DHHS
  • Comprehensive Cancer Center Core Support Grant - Tissue Procurement Shared Facility  awarded by National Cancer Institute/NIH/DHHS
  • Comprehensive Cancer Center Core Support Grant - Tissue Procurement Shared Facility  awarded by National Cancer Institute/NIH/DHHS
  • Cooperative Human Tissue Network  awarded by National Cancer Institute/NIH/DHHS
  • Morehouse School of Medicine/Tuskegee University/University of Alabama Cancer Center Partnership: Bioethics Shared Resource  awarded by National Cancer Institute/NIH/DHHS
  • Multicenter Genetic Epigenetic & Expression Analysis of DCIS Outcome Predictors  awarded by Johns Hopkins University
  • Novel Sulindac Derivatives for Colon Cancer Chemoprevention  awarded by University of South Alabama
  • PHBI Transition  awarded by Indiana University
  • Phosphodiesterase 5: A Novel Target and Inhibitor for Breast Cancer Chemoprevention  awarded by University of South Alabama
  • Private Grant  awarded by ROCKLAND IMMUNOCHEMICALS
  • Private Grant  awarded by LEIDOS BIOMEDICAL RESEARCH, INC.
  • Private Grant  awarded by LEIDOS BIOMEDICAL RESEARCH, INC.
  • Private Grant  awarded by LEIDOS BIOMEDICAL RESEARCH, INC.
  • Pulmonary Hypertension Breakthrough Initiative  awarded by University of Colorado
  • Pulmonary Hypertension Breakthrough Initiative  awarded by Indiana University
  • SPORE in Breast Cancer - Tissue Core  awarded by National Cancer Institute/NIH/DHHS
  • The Importance of ENT in Breast Cancer in African American Women  awarded by George Washington University
  • Theranostic Nanoparticles For Detection and Treatment of Pancreatic Cancer  awarded by BOARD OF TRUSTEES OF THE UNIVERSITY OF OKLAHOMA HEALTH SCIENCES CENTER
  • Theranostic Nanoparticles for Detection and Treatment of Pancreatic Cancer  awarded by WAKE FOREST UNIVERSITY
  • Tissue Resource Site (TSS) in Support of the The Cancer Genome Atlas (TCGA) Program - Admin Core  awarded by SAIC - FREDERICK
  • Tissue Resource Site (TSS) in Support of the The Cancer Genome Atlas (TCGA) Program - Task Order 1  awarded by SAIC - FREDERICK
  • Tissue Source Site (TSS) in Support of the NCI Clinical Assay Development Program - Admin Core  awarded by SAIC - FREDERICK
  • Transcriptional Repressor Kaiso Promotes Metastasis through Epithelial to Mesenchymal Transition  awarded by TUSKEGEE UNIVERSITY
  • UAB / UMN SPORE in Pancreatic Cancer - Core B  awarded by National Cancer Institute/NIH/DHHS
  • UAB Skin Disease Research Center - Tissue Resources and Molecular Pathology Core - Core C  awarded by National Institute of Arthritis & Musculoskeletal & Skin Diseases/NIH/DHHS
  • Investigator On

  • 3/3 Morehouse School of Medicine/Tuskegee University/University of Alabama Cancer Center Partnership  awarded by National Cancer Institute/NIH/DHHS
  • A Specific Screening Strategy to Reduce Prostate Cancer Mortality  awarded by DOD - ARMY MEDICAL RESEARCH ACQUISITION ACTIVITY
  • Breast Tumor Tissue-Derived Hedgehog Morphogens Immunoedit the Polarization of Breast Cancer Associated Macrophages  awarded by DOD - ARMY MEDICAL RESEARCH ACQUISITION ACTIVITY
  • Comprehensive Cancer Center Core Support Grant  awarded by National Cancer Institute/NIH/DHHS
  • Comprehensive Cancer Center Core Support Grant  awarded by National Cancer Institute/NIH/DHHS
  • Comprehensive Cancer Center Core Support Grant  awarded by National Cancer Institute/NIH/DHHS
  • Comprehensive Cancer Center Core Support Grant  awarded by National Cancer Institute/NIH/DHHS
  • Comprehensive Cancer Center Core Support Grant  awarded by National Cancer Institute/NIH/DHHS
  • Exploring Effects of Weight Loss on Ductal Carcinoma In Situ  awarded by National Cancer Institute/NIH/DHHS
  • Glycan Control of Stem Cell-Associated Pathways in Pancreatic Cancer  awarded by National Cancer Institute/NIH/DHHS
  • Glycosylation-Dependent Mechanisms Regulating Ovarian Tumor Cell Survival  awarded by National Institute of General Medical Sciences/NIH/DHHS
  • Hepato/Renal Fibrocystic Diseases Core Center (UAB HRFDCC)  awarded by National Institute of Diabetes and Digestive and Kidney Diseases/NIH/DHHS
  • Hepato/Renal Fibrocystic Diseases Core Center (UAB HRFDCC) - Admin Core  awarded by National Institute of Diabetes and Digestive and Kidney Diseases/NIH/DHHS
  • Hepato/Renal Fibrocystic Diseases Core Center (UAB HRFDCC) - Core A  awarded by National Institute of Diabetes and Digestive and Kidney Diseases/NIH/DHHS
  • Hepato/Renal Fibrocystic Diseases Core Center (UAB HRFDCC) - Core A  awarded by National Institute of Diabetes and Digestive and Kidney Diseases/NIH/DHHS
  • Mechanisms of Resistance to Cancer Therapeutics  awarded by National Cancer Institute/NIH/DHHS
  • Molecular Pathogenesis and Phenotype of Acquired CFTR Dysfunction in COPD  awarded by National Heart, Lung, and Blood Institute/NIH/DHHS
  • Morehouse School of Medicine/Tuskegee University/University of Alabama Cancer Center Partnership  awarded by National Cancer Institute/NIH/DHHS
  • Networks of Tissue Source Site (TSS) in Support of the Cancer Genome Atlas (TCGA) Program  awarded by Washington University
  • Private Grant  awarded by LEIDOS BIOMEDICAL RESEARCH, INC.
  • SPORE in Breast Cancer  awarded by National Cancer Institute/NIH/DHHS
  • Sialylation-dependent Mechanisms Driving Pancreatic Cancer Progression  awarded by National Cancer Institute/NIH/DHHS
  • TGFBR1 Signaling in Colorectal Cancer  awarded by National Cancer Institute/NIH/DHHS
  • The Hepato/Renal Fibrocystic Disease Core Center (UAB HRFDCC)  awarded by National Institute of Diabetes and Digestive and Kidney Diseases/NIH/DHHS
  • UAB / UMN SPORE in Pancreatic Cancer  awarded by National Cancer Institute/NIH/DHHS
  • UAB / UMN SPORE in Pancreatic Cancer - Project 1  awarded by National Cancer Institute/NIH/DHHS
  • UAB NRG Oncology Member Institution Purchased Service Agreement  awarded by NRG ONCOLOGY FOUNDATION
  • UAB Skin Disease Research Center  awarded by National Institute of Arthritis & Musculoskeletal & Skin Diseases/NIH/DHHS
  • Education And Training

  • Vanderbilt University Medical Center, Internship
  • UAB Hospital, Residency
  • Doctor of Medicine, Johns Hopkins University 1977
  • Doctor of Philosophy in Biophysics, Johns Hopkins University 1975
  • Full Name

  • William Grizzle