Positions

Overview

  • My lab aims to understand virus structure and the structure-function relationships involved in the processes of viral replication, including virus-host interactions, capsid structure and polynucleotide synthesis. Toward this goal, we have used structural techniques (cryo-electron microscopy, x-ray crystallography and small angle x-ray scattering) alongside biochemistry and classical virology techniques to study viral proteins from negative strand RNA viruses (NSV: influenza A, mumps, rabies virus, VSV) and retroviruses (HIV). This dual approach has been successful in producing near atomic-level snapshots of many complex protein assemblies as well as enabling a fast-track to novel biological discovery and new experimental design. Professionally, my expertise in structural biology has lead to my collaboration with investigators locally, nationally and internationally on viral, bacterial, human and murine targets.
  • Selected Publications

    Academic Article

    Year Title Altmetric
    2018 Structural analyses reveal the mechanism of inhibition of influenza virus NS1 by two antiviral compounds.Journal of Biological Chemistry.  293:14659-14668. 2018
    2018 Mutations in Escherichia coli Polyphosphate Kinase That Lead to Dramatically Increased In Vivo Polyphosphate Levels.Journal of Bacteriology.  200. 2018
    2016 Signature motifs of GDP polyribonucleotidyltransferase, a non-segmented negative strand RNA viral mRNA capping enzyme, domain in the L protein are required for covalent enzyme-pRNA intermediate formation.Nucleic Acids Research.  44:330-341. 2016
    2016 Crystal Structures of Group B Streptococcus Glyceraldehyde-3-Phosphate Dehydrogenase: Apo-Form, Binary and Ternary Complexes.PLoS ONE.  11:e0165917. 2016
    2015 Structure and Function of the N-Terminal Domain of the Vesicular Stomatitis Virus RNA Polymerase.Journal of Virology.  90:715-724. 2015
    2014 1.55 Å resolution X-ray crystal structure of Rv3902c from Mycobacterium tuberculosis.Acta Crystallographica Section F: Structural Biology Communications.  70:414-417. 2014
    2014 Common mechanism for RNA encapsidation by negative-strand RNA viruses.Journal of Virology.  88:3766-3775. 2014
    2013 Structural and functional characterization of the mumps virus phosphoprotein.Journal of Virology.  87:7558-7568. 2013
    2013 Nucleocapsid protein structures from orthobunyaviruses reveal insight into ribonucleoprotein architecture and RNA polymerization.Nucleic Acids Research.  41:5912-5926. 2013
    2011 Access to RNA encapsidated in the nucleocapsid of vesicular stomatitis virus.Journal of Virology.  85:2714-2722. 2011
    2010 Structure of human stabilin-1 interacting chitinase-like protein (SI-CLP) reveals a saccharide-binding cleft with lower sugar-binding selectivity.Journal of Biological Chemistry.  285:39898-39904. 2010
    2010 Cryo-EM model of the bullet-shaped vesicular stomatitis virus.Science.  327:689-693. 2010
    2009 Characterization of a mumps virus nucleocapsidlike particle.Journal of Virology.  83:11402-11406. 2009
    2009 Structure of the vesicular stomatitis virus nucleocapsid in complex with the nucleocapsid-binding domain of the small polymerase cofactor, P.Proceedings of the National Academy of Sciences.  106:11713-11718. 2009
    2009 Crystallization and preliminary X-ray crystallographic studies on SI-CLP, a novel human Glyco_18 domain-containing protein.Protein and Peptide Letters.  16:336-338. 2009
    2008 Structural and functional insights into the molecular mechanisms responsible for the regulation of pyruvate dehydrogenase kinase 2.Journal of Biological Chemistry.  283:15789-15798. 2008
    2008 Role of intermolecular interactions of vesicular stomatitis virus nucleoprotein in RNA encapsidation.Journal of Virology.  82:674-682. 2008
    2007 Conserved characteristics of the rhabdovirus nucleoprotein.Virus Research.  129:246-251. 2007
    2007 Structural comparisons of the nucleoprotein from three negative strand RNA virus families.Virology Journal.  4:72. 2007
    2006 Structure of the vesicular stomatitis virus nucleoprotein-RNA complex.Science.  313:357-360. 2006
    2006 Resolution improvement of X-ray diffraction data of crystals of a vesicular stomatitis virus nucleocapsid protein oligomer complexed with RNA.Acta Crystallographica Section D: Biological Crystallography.  62:498-504. 2006
    2006 Purification, crystallization and preliminary X-ray crystallographic analysis of the nucleocapsid protein of Bunyamwera virus.Acta Crystallographica Section F: Structural Biology and Crystallization Communications.  62:361-364. 2006
    2006 Crystal structure of the oligomerization domain of the phosphoprotein of vesicular stomatitis virus.Journal of Virology.  80:2808-2814. 2006
    2004 Crystallization and preliminary X-ray analysis of a proteinase-K-resistant domain within the phosphoprotein of vesicular stomatitis virus (Indiana).Acta Crystallographica Section D: Biological Crystallography.  60:2087-2090. 2004
    2004 Visualizing the RNA molecule in the bacterially expressed vesicular stomatitis virus nucleoprotein-RNA complex.Structure.  12:227-235. 2004
    2003 Expression, purification, crystallization of fragments from the C-terminal region of DFF45/ICAD.Acta Crystallographica Section D: Biological Crystallography.  59:1323-1326. 2003
    2000 Study of the assembly of vesicular stomatitis virus N protein: role of the P protein.Journal of Virology.  74:9515-9524. 2000
    1997 Role of sialyloligosaccharide binding in Theiler's virus persistence.Journal of Virology.  71:9701-9712. 1997
    1995 A Cytotoxic Diacetylene from Dendropanax arboreusPlanta Medica.  61:470-471. 1995
    1995 An Antibacterial Vitamin E Derivative from Tovomitopsis psychotriifoliaPlanta Medica.  61:275-276. 1995

    Chapter

    Year Title Altmetric
    2011 Assembly of vesicular stomatitis virus.  175-192. 2011

    Research Overview

  • The targets of my current NIH funded research are to understand the processes of NSV polynucleotide synthesis from the view of the template (a complex between the viral genome and the nucleocapsid) as well as the polymerase machinery (a complex between the L protein, P protein and potentially host factors).
  • Education And Training

  • Bachelor of Science or Mathematics in Biology, University of Alabama in Huntsville
  • Doctor of Philosophy in Microbiology, University of Alabama at Birmingham
  • Full Name

  • Todd Green
  • Blazerid

  • tgreen