Centriole duplication in lysates of Spisula solidissima oocytes

Academic Article

Abstract

  • A cell-free system has been developed that executes centriole duplication. Surf clam (Spisula solidissima) oocytes, arrested at late prophase of meiosis I, do not contain centrioles, centrosomes, or asters. Serial section high-voltage electron microscopy (HVEM) of asters and spindles isolated from potassium chloride-activated oocytes ind-cates that within 4 minutes oocytes assemble a single centriole that is duplicated by 15 minutes when assembly of the first meiotic spindle is complete. A mixture of lysates from unactivated oocytes and potassium chloride-activated oocytes induces centriole formation and duplication. Astral microtubule content in these lysate mixtures increases with time.
  • Authors

    Published In

  • Science  Journal
  • Digital Object Identifier (doi)

    Author List

  • Palazzo RE; Vaisberg E; Cole RW; Rieder CL
  • Start Page

  • 219
  • End Page

  • 221
  • Volume

  • 256
  • Issue

  • 5054