Molecular cloning of a cDNA encoding putative molt-inhibiting hormone from the blue crab, callinectes sapidus

Academic Article

Abstract

  • A cDNA library was constructed using poly(A+) RNA isolated from eyestalk neural ganglia of the blue crab, Callinectes sapidus. The library was screened using a probe generated by PCR based on the published amino acid sequence of molt-inhibiting hormone from the shore crab, Carcinus maenas. DNA sequence analysis of one positive clone revealed a 339 bp open reading frame encoding a 78-residue putative molt-inhibiting hormone and a 35-residue signal peptide. The deduced amino acid sequence of C. sapidus molt-inhibiting hormone is 79% homologous with that of C. maenas. Northern blot analysis, using a fragment of the cloned molt-inhibiting hormone cDNA as probe, revealed specific hybridization to a single band (∼1.4 kb) in RNA extracted from eyestalk but not control tissues. © 1995 by Academic Press, Inc.
  • Digital Object Identifier (doi)

    Author List

  • Lee KJ; Elton TS; Bej AK; Watts SA; Douglas Watson R
  • Start Page

  • 1126
  • End Page

  • 1131
  • Volume

  • 209
  • Issue

  • 3