Inter- and intra-strain variability of tandem repeats in Mycoplasma pneumoniae based on next-generation sequencing data.

Academic Article


  • AIM: To characterize inter- and intra-strain variability of variable-number tandem repeats (VNTRs) in Mycoplasma pneumoniae to determine the optimal multilocus VNTR analysis scheme for improved strain typing. METHODS: Whole genome assemblies and next-generation sequencing data from diverse M. pneumoniae isolates were used to characterize VNTRs and their variability, and to compare the strain discriminability of new VNTR and existing markers. RESULTS: We identified 13 VNTRs including five reported previously. These VNTRs displayed different levels of inter- and intra-strain copy number variations. All new markers showed similar or higher discriminability compared with existing VNTR markers and the P1 typing system. CONCLUSION: Our study provides novel insights into VNTR variations and potential new multilocus VNTR analysis schemes for improved genotyping of M. pneumoniae.
  • Published In


  • Mycoplasma pneumoniae, next-generation sequencing, tandem repeats, DNA Copy Number Variations, DNA, Bacterial, Genetic Loci, Genetic Markers, Genotyping Techniques, High-Throughput Nucleotide Sequencing, Minisatellite Repeats, Mycoplasma pneumoniae, Sequence Analysis, DNA
  • Digital Object Identifier (doi)

    Author List

  • Zhang J; Song X; Ma MJ; Xiao L; Kenri T; Sun H; Ptacek T; Li S; Waites KB; Atkinson TP
  • Start Page

  • 119
  • End Page

  • 129
  • Volume

  • 12