Na-K-Cl cotransport in villus and crypt cells from rat duodenum

Academic Article

Abstract

  • The aim of the present study was to examine the possibility that Cl - uptake into both villus and crypt epithelial cells of rat duodenum occurs via an electroneutral Na-K-Cl coupled-transport mechanism. Sheets of villus cells and whole crypts were isolated using a Ca 2+ chelation technique combined with continuous vibration at low temperatures. Structurally intact, viable epithelia from defined regions along the villus-crypt axis were produced. Uptake of 86 Rb + (as a proxy for K + ) into both villus and crypt cells appeared to depend on a coupled process, as evidenced by the inhibition of 86 Rb + uptake by bumetanide and by the removal of either Na + or Cl - from the bathing media. We report an improved method of isolation of viable enterocytes from defined regions along the villus-crypt axis. We demonstrate the presence of Na-K-Cl cotransport in both villus and crypt duodenal enterocytes.
  • Author List

  • McNicholas CM; Brown CDA; Turnberg LA
  • Volume

  • 267
  • Issue

  • 6 30-6