Na-K-Cl cotransport in villus and crypt cells from rat duodenum.

Academic Article

Abstract

  • The aim of the present study was to examine the possibility that Cl- uptake into both villus and crypt epithelial cells of rat duodenum occurs via an electroneutral Na-K-Cl coupled-transport mechanism. Sheets of villus cells and whole crypts were isolated using a Ca2+ chelation technique combined with continuous vibration at low temperatures. Structurally intact, viable epithelia from defined regions along the villus-crypt axis were produced. Uptake of 86Rb+ (as a proxy for K+) into both villus and crypt cells appeared to depend on a coupled process, as evidenced by the inhibition of 86Rb+ uptake by bumetanide and by the removal of either Na+ or Cl- from the bathing media. We report an improved method of isolation of viable enterocytes from defined regions along the villus-crypt axis. We demonstrate the presence of Na-K-Cl cotransport in both villus and crypt duodenal enterocytes.
  • Published In

    Keywords

  • Animals, Biological Transport, Carrier Proteins, Cell Survival, Chlorides, Duodenum, Male, Potassium, Rats, Rats, Sprague-Dawley, Rubidium Radioisotopes, Sodium, Sodium-Potassium-Chloride Symporters
  • Digital Object Identifier (doi)

    Author List

  • McNicholas CM; Brown CD; Turnberg LA
  • Start Page

  • G1004
  • End Page

  • G1011
  • Volume

  • 267
  • Issue

  • 6 Pt 1