Primary structure and functional expression of a cGMP-gated potassium channel

Academic Article

Abstract

  • Cyclic nucleotides modulate potassium (K) channel activity in many cells and are thought to act indirectly by inducing channel protein phosphorylation. Herein we report the isolation from rabbit of a gene encoding a K channel (Kcn1) that is specifically activated by cGMP and not by cAMP. Analysis of the deduced amino acid sequence (725 amino acids) indicates that, in addition to a core region that is highly homologous to Shaker K channels, Kcn1 also contains a cysteine-rich region similar to that of ligand-gated ion channels and a cyclic nucleotide-binding region. Northern blot analysis detects gene expression in kidney, aorta, and brain. Kcn1 represents a class of K channels that may be specifically regulated by cGMP and could play an important role in mediating the effects of substances, such as nitric oxide, that increase intracellular cGMP.
  • Digital Object Identifier (doi)

    Author List

  • Yao X; Segal AS; Welling P; Zhang X; Mcnicholas CM; Engel D; Boulpaep EL; Desir GV
  • Start Page

  • 11711
  • End Page

  • 11715
  • Volume

  • 92
  • Issue

  • 25