This paper investigates the calibration effect of urine biomarkers by creatinine. The biomarkers were obtained from each urine sample of 256 patients (cancer: 137, benign: 119). The logistic regression of the combinations of 2 biomarkers calibrated by creatinine concentration was compared with that of the 3 biomarkers including creatinine. The average AUC over 1000 rounds of 5-fold cross validations was employed to evaluate the performance. The logistic regression of the calibrated biomarkers showed better performance than that without calibration for the top- ranked combinations. © 2014 SERSC.