Ontogeny of Ia and IgD on IgM-bearing B lymphocytes in mice.

Academic Article


  • We used immunofluorescence to examine the developmental relationship of Ia and IgD on B cells. Pre-B cells in fetal liver did not express Ia. Only very few surface IgM-positive (sIgM+) B cells in fetal spleen were found to be Ia+ and were weakly stained for Ia. After birth there was a linear increase in the proportion of sIgM+ spleen cells which expressed Ia, reaching 95% by 9 days. Adult bone marrow also contains a sizeable proportion of sIgM+ Ia- cells. Unstimulated cells from fetal or newborn liver and spleen expressed Ia at the same rate in culture. Anti-Ia antisera suppressed the LPS-induced differentiation of IgM and IgG plasma cells in cultures of neonatal lymphocytes. Ia was also detected on IgM and IgG plasma cells in vitro suggesting that lipopolysaccharide (LPS)-stimulated B cells by may express Ia antigens, induced by LPS, or appearing as part of normal differentiation. IgD did not appear on sIgM+ cells until 3 days of age and then rose slowly to reach adult levels later than Ia antigens.
  • Published In


  • Aging, Animals, B-Lymphocytes, Bone Marrow, Bone Marrow Cells, Cell Division, Histocompatibility Antigens, Immunoglobulin D, Immunoglobulin M, Lipopolysaccharides, Liver, Mice, Mice, Inbred Strains, Plasma Cells, Receptors, Antigen, B-Cell, Spleen
  • Author List

  • Kearney JF; Cooper MD; Klein J; Abney ER; Parkhouse RM; Lawton AR
  • Start Page

  • 297
  • End Page

  • 301
  • Volume

  • 146
  • Issue

  • 1