Cellular expression of idiotopes defined by monoclonal antibodies.

Academic Article

Abstract

  • Monoclonal anti-idiotype (Id) antibodies with specificities for determinants related to the antigen-binding sites of 3 BALB/c myeloma proteins, MOPC-460, HOPC-8 and J558, were used to study Id expression on murine lymphocytes. The monoclonal antibodies were shown to react only with Id structures associated with immunoglobulin on B cells. None of these 3 Id nor a VH Id, detected by a monoclonal antibody made against HOPC-8 heavy chain, were found on T cells. These Id were detected on splenic B cells in neonatal mice; the frequencies in normal, nude and germ-free mice were similar: MOPC-460 Id+: 1.05 +/- 1.7/10(4) spleen cells, HOPC-8 Id+: 1.45 +/- 1.2/10(4) and J558: 0.35 +/- 0.6/10(4). Almost all Id+ cells bore surface IgM, a few expressed surface IgG. MOPC-460 Id+ IgG+ cells were mainly gamma 2a+ or gamma 2b+, whereas J558 and HOPC-8 Id+ IgG+ cells were gamma 3+.
  • Published In

    Keywords

  • Aging, Animals, Antibodies, Monoclonal, Cell Line, Epitopes, Immunoglobulin Idiotypes, Lymph Nodes, Lymphocytes, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Mice, Inbred Strains, Plasmacytoma, Species Specificity, Spleen
  • Digital Object Identifier (doi)

    Author List

  • Bast BJ; Cooper MD; Kearney JF
  • Start Page

  • 623
  • End Page

  • 628
  • Volume

  • 14
  • Issue

  • 7