A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines.

Academic Article

Abstract

  • We have isolated a subclone of the mouse myeloma cell line P3-X63-Ag8 that does not express immunoglobulin heavy or light chains. This clone X63-Ag8.653 can be used for efficient fusion with antibody-forming cells to obtain hybrid cell lines producing pure monoclonal antibodies. Screening of hybrid cell lines for specificity and immunoglobulin classes was done with a modified enzyme-linked immunosorbent assay.
  • Published In

    Keywords

  • Animals, Antibody-Producing Cells, Cell Fusion, Cell Line, Cell Transformation, Neoplastic, Enzyme-Linked Immunosorbent Assay, Hybrid Cells, Immunoglobulin Heavy Chains, Immunoglobulin Light Chains, Mice, Mice, Inbred C57BL, Multiple Myeloma, Rabbits
  • Author List

  • Kearney JF; Radbruch A; Liesegang B; Rajewsky K
  • Start Page

  • 1548
  • End Page

  • 1550
  • Volume

  • 123
  • Issue

  • 4