Isotype specificity of helper T cell clones. Peyer's patch Th cells preferentially collaborate with mature IgA B cells for IgA responses.

Academic Article

Abstract

  • The nature of the IgA B cell precursors that receive preferential help from selected clones of T helper cells from mouse Peyer's patches (PP Th A) were studied. Activation of the PP Th A clones required the presence of antigen, sheep erythrocytes (SRBC), in a culture system supporting development of antibody-secreting plasma cells. Two types of PP Th A cells were used. Both gave vigorous IgA responses; the first also supported low IgM, and the second low IgM and IgG subclass antibody responses. Removal of sIgA+ B cells from either splenic or PP B cell cultures selectively depleted precursors of IgA antibody producers. Cultures of purified sIgA+ B cells, cloned PP Th A cells and SRBC, selectively yielded IgA antibody producers. Finally, PP Th A cells did not support IgA responses in B cell cultures derived from spleens of young mice (days 1-25), and full IgA responses were not seen until the donor mice were 6-7 wk of age. These results suggest that cloned T helper cells can recognize and collaborate with mature, IgA committed B cells.

    • Authors

    Published In

    Keywords

  • Animals, Antibody-Producing Cells, B-Lymphocytes, Cell Differentiation, Clone Cells, Immunoglobulin A, Immunoglobulin Allotypes, Lymphocyte Cooperation, Mice, Mice, Inbred C3H, Peyer's Patches, Receptors, Antigen, B-Cell, Spleen, T-Lymphocytes, Helper-Inducer

    • Author List

  • Kiyono H; Cooper MD; Kearney JF; Mosteller LM; Michalek SM; Koopman WJ; McGhee JR

    • Start Page

  • 798

    • End Page

  • 811

    • Volume

  • 159

    • Issue

  • 3