Microbicidal power of alpha radiation in sterilizing germinating Bacillus anthracis spores.

Academic Article

Abstract

  • Radioimmunotherapy (RIT) takes advantage of the specificity and affinity of the antigen-antibody interaction to deliver microbicidal radioactive nuclides to a site of infection. In this study, we investigated the microbicidal properties of an alpha particle-emitting 213Bi-labeled monoclonal antibody (MAb), EA2-1 (213Bi-EA2-1), that binds to the immunodominant antigen on Bacillus anthracis spores. Our results showed that dormant spores were resistant to 213Bi-EA2-1. Significant spore killing was observed following treatment with EA2-1 labeled with 300 ╬╝Ci 213Bi; however, this effect was not dependent on the MAb. In contrast, when spores were germinating, 213Bi-EA2-1 mediated MAb-specific killing in a dose-dependent manner. Dormant spores are very resistant to RIT, and RIT should focus on targeting vegetative cells and germinating spores.
  • Published In

    Keywords

  • Alpha Particles, Antibodies, Monoclonal, Bacillus anthracis, Bismuth, Microscopy, Phase-Contrast, Radioisotopes, Spores, Bacterial
  • Digital Object Identifier (doi)

    Author List

  • Rivera J; Morgenstern A; Bruchertseifer F; Kearney JF; Turnbough CL; Dadachova E; Casadevall A
  • Start Page

  • 1813
  • End Page

  • 1815
  • Volume

  • 58
  • Issue

  • 3