Autoregulation of afferent arteriolar blood flow in juxtamedullary nephrons.

Academic Article

Abstract

  • Utilizing the in vitro blood-perfused juxtamedullary nephron preparation, we examined the effects of alterations in renal arterial pressure on afferent arteriolar blood flow. With video microscopy and cross-correlation techniques, arteriolar inside diameters and centerline erythrocyte velocity were measured to estimate single afferent arteriolar blood flow. In response to random changes in perfusion pressure, afferent arteriolar diameter (n = 8) varied inversely (-0.53 +/- 0.02%/mmHg), and erythrocyte velocity was directly related (1.4 +/- 0.1%/mmHg). Above 95 mmHg, the slope of the relationship between perfusion pressure and afferent arteriolar blood flow did not differ from zero (0.081 +/- 0.053%/mmHg), suggesting efficient autoregulation. When the tubuloglomerular feedback pathway was interrupted by the addition of furosemide (n = 9) or papillectomy (n = 7), there was attenuation of pressure-induced afferent arteriolar constriction, with impairment in blood flow autoregulation (0.60 +/- 0.05%/mmHg). Superfusion with diltiazem abolished autoregulatory responses in afferent arteriolar diameter and blood flow (1.5 +/- 0.2%/mmHg). These data demonstrate the autoregulation of blood flow of individual afferent arterioles in juxtamedullary nephrons and suggest that both tubuloglomerular feedback-dependent and -independent mechanisms are required for autoregulatory responses.
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    Keywords

  • Animals, Arterioles, Blood Flow Velocity, Blood Pressure, Feedback, Homeostasis, Juxtaglomerular Apparatus, Kidney Medulla, Male, Muscle, Smooth, Vascular, Nephrons, Rats, Rats, Sprague-Dawley, Regional Blood Flow, Renal Circulation
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    Author List

  • Takenaka T; Harrison-Bernard LM; Inscho EW; Carmines PK; Navar LG
  • Start Page

  • F879
  • End Page

  • F887
  • Volume

  • 267
  • Issue

  • 5 Pt 2