Interactive nitric oxide-angiotensin II influences on renal microcirculation in angiotensin II-induced hypertension

Academic Article

Abstract

  • The present study was conducted to determine the contribution of nitric oxide to angiotensin II (Ang II) reactivity of afferent and efferent arterioles from Ang II-infused hypertensive rats. Experiments were performed in vitro with the blood-perfused juxtamedullary nephron technique in kidneys harvested from hypertensive Sprague-Dawley rats (181 ± 1 mm Hg) that had received 60 ng/min Ang II subcutaneously for 13 days. Superfusion with 0.1, 1, and 10 nmol/L Ang II reduced afferent arteriolar diameter (18.1 ± 0.6 μm; n=12) by 10.0 ± 0.7%, 28.1 ± 1.7%, and 52.8 ±1.9%, respectively, and efferent arteriolar diameter (17.2 ± 1.4 μm; n=8) decreased by 9.3 ± 0.7%, 27.0 ± 1.2%, and 50.4 ± 1.6%, respectively. Nitric oxide synthase inhibition with 100 μmol/L N(ω)-nitro-L-arginine (NA) reduced resting afferent and efferent arteriolar diameters to 14.7 ± 0.4 and 14.3 ± 1.2 μm, respectively, and enhanced afferent but not efferent arteriolar reactivity to Ang II. The enhanced afferent arteriolar reactivity to Ang II was eliminated by addition of the nitric oxide donor S-nitroso-N- acetylpenicillamine (SNAP, 10 μmol/L), which reversed the NA-induced decrease in diameter. Addition of 10 μmol/L SNAP, without NA, blunted efferent but not afferent arteriolar reactivity to Ang II. Afferent (n=7) and efferent arteriolar diameters (n=6) decreased by 48.5 ± 2.2% and 41.0 ± 1.9%, respectively, in response to 10 nmol/L Ang II. These results suggest that in this model of hypertension, maintained nitric oxide production in afferent arterioles counteracts the enhanced afferent arteriolar reactivity that occurs in Ang II-induced hypertension.
  • Authors

    Published In

  • Hypertension  Journal
  • Digital Object Identifier (doi)

    Author List

  • Ichihara A; Imig JD; Inscho EW; Navar LG
  • Start Page

  • 1255
  • End Page

  • 1260
  • Volume

  • 31
  • Issue

  • 6