Structure of insect-cell-derived IL-22

Academic Article

Abstract

  • The crystal structure of interleukin-22 expressed in Drosophila melanogaster S2 cells (IL-22Dm) has been determined at 2.6 Å resolution. IL-22Dm crystals contain six molecules in the asymmetric unit. Comparison of IL-22Dm and IL-22Ec (interleukin-22 produced in Escherichia coli) structures reveals that N-linked glycosylation causes only minor structural changes to the cytokine. However, 1-4 Å main-chain differences are observed between the six IL-22Dm monomers at regions corresponding to the IL-22R1 and IL-10R2 binding sites. The structure of the carbohydrate and the conformational variation of IL22Dm provide new insights into IL-22 receptor recognition. © 2005 International Union of Crystallography - all rights reserved.
  • Digital Object Identifier (doi)

    Author List

  • Xu T; Logsdon NJ; Walter MR
  • Start Page

  • 942
  • End Page

  • 950
  • Volume

  • 61
  • Issue

  • 7