TRPC3 channels are necessary for brain-derived neurotrophic factor to activate a nonselective cationic current and to induce dendritic spine formation

Academic Article


  • Brain-derived neurotrophic factor (BDNF) exerts prominent effects on hippocampal neurons, but the mechanisms that initiate its actions are poorly understood. We report here that BDNF evokes a slowly developing and sustained nonselective cationic current (IBDNF) inCA1 pyramidal neurons. These responses require phospholipase C, IP3 receptors, Ca 2+stores, and Ca2+influx, suggesting the involvement of transient receptor potential canonical subfamily (TRPC) channels. Indeed, I BDNF is absent after small interfering RNA-mediated TRPC3 knockdown. The sustained kinetics of IBDNF appears to depend on phosphatidylinositol 3-kinase-mediated TRPC3 membrane insertion, as shown by surface biotinylation assays. Slowly emerging membrane currents after theta burst stimulation are sensitive to the scavenger TrkB-IgG and TRPC inhibitors, suggesting IBDNF activation by evoked released of endogenous, native BDNF. Last, TRPC3 channels are necessary for BDNF to increase dendritic spine density. Thus, TRPC channels emerge as novel mediators of BDNF-mediated dendritic remodeling through the activation of a slowly developing and sustained membrane depolarization. Copyright © 2007 Society for Neuroscience.
  • Digital Object Identifier (doi)

    Author List

  • Amaral MD; Pozzo-Miller L
  • Start Page

  • 5179
  • End Page

  • 5189
  • Volume

  • 27
  • Issue

  • 19