The development of α2-adrenoceptors in the rat kidney: Correlation with noradrenergic innervation

Academic Article

Abstract

  • The development of the renal α2-adrenoceptors in the rat was studied by binding renal membranes of prenatal and postnatal rats with p-[3,5-3H]aminoclonidine (PAC), a selective α2-adrenoceptor agonist. The results demonstrate that prior to birth and on the day of birth [3H]PAC binding is present and reflects a single linear binding affinity (Kd {equal to or succeeds} 1.0 nM). In contrast, data from postnatal day 3 demonstrate the addition of a second, lower affinity binding site (Kd {equal to or succeeds} 7.0 nM) that develops rapidly during the ensuing weeks of life. Both high and low affinity α2-adrenoceptor binding sites continue to increase in receptor density through 30 weeks of age; however, the binding affinity of each site remains relatively stable. The sympathetic noradrenergic innervation of the rat kidney is present prenatally and rapidly develops during the first 3 weeks of life with peak noradrenaline concentrations being reached at approximately postnatal day 21. Immunohistochemical staining demonstrates that tyrosine hydroxylase-positive axons rapidly develop in the kidney during the same period, indicating that the increase in noradrenaline is related to expansion of renal sympathetic innervation. © 1987.
  • Authors

    Published In

  • Brain Research  Journal
  • Digital Object Identifier (doi)

    Author List

  • Sripanidkulchai B; Wyss JM
  • Start Page

  • 91
  • End Page

  • 100
  • Volume

  • 400
  • Issue

  • 1