Genistein and biochanin A inhibit the growth of human prostate cancer cells but not epidermal growth factor receptor tyrosine autophosphorylation.

Academic Article

Abstract

  • The effect of the isoflavones, genistein, daidzein, and biochanin A on the growth of the LNCaP and DU-145 human prostate cancer cell lines has been examined. Genistein and biochanin A, but not daidzein, inhibit both serum and EGF-stimulated growth of LNCaP and DU-145 cells (IC50 values from 8.0 to 27 micrograms/ml for serum and 4.3 to 15 micrograms/ml for EGF), but have no significant effect of the EGF receptor tyrosine autophosphorylation. In contrast, tyrphostin 25, a specific EGF receptor tyrosine kinase inhibitor, inhibits EGF-stimulated growth and EGF receptor tyrosine autophosphorylation in these whole cells, but does not inhibit serum-stimulated growth. These data suggest that the mechanism of action of genistein and biochanin A does not depend on inhibition of EGF receptor tyrosine autophosphorylation, but on a more distal event in the EGF receptor-mediated signal transduction cascade.
  • Published In

  • Prostate  Journal
  • Keywords

  • Anticarcinogenic Agents, Catechols, Cell Division, Epidermal Growth Factor, ErbB Receptors, Estrogens, Non-Steroidal, Genistein, Humans, Isoflavones, Male, Nitriles, Phosphorylation, Prostatic Neoplasms, Protein-Tyrosine Kinases, Tumor Cells, Cultured, Tyrosine, Tyrphostins
  • Author List

  • Peterson G; Barnes S
  • Start Page

  • 335
  • End Page

  • 345
  • Volume

  • 22
  • Issue

  • 4