Synthesis, conjugation, and immunological evaluation of the serogroup 6 pneumococcal oligosaccharides

Academic Article

Abstract

  • The first synthesis of the newly discovered oligosaccharide of pneumococcal serotype 6C and its spacer-containing analogue is reported. Conjugation of the spacer-containing oligosaccharides of pneumococcal saccharides 6A, 6B, 6C and derivatives thereof with bovine serum albumin (BSA) protein carrier was carried out by using squaric-acid approach to obtain the oligosaccharide-protein conjugates in excellent yields. The conjugates have been tested with a rabbit antiserum pool (Pool B) used for pneumococcal serotyping. The results showed that synthetic carbohydrate conjugates express epitopes found in native capsular polysaccharides of serotypes 6A, 6B, and 6C. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.
  • Authors

    Digital Object Identifier (doi)

    Author List

  • Parameswar AR; Park IH; Saksena R; Kovác̈ P; Nahm MH; Demchenko AV
  • Start Page

  • 2893
  • End Page

  • 2899
  • Volume

  • 10
  • Issue

  • 18