Identification of 3-O-acetylglycerol, a novel structural element in bacterial polysaccharides

Academic Article

Abstract

  • We have discovered a novel bacterial polysaccharide structural element, 3-O-acetylglycerol, in the Streptococcus pneumoniae ST11A polysaccharide:{A figure is presented}. This moiety was elucidated through a combination of homonuclear and heteronuclear 1D and 2D NMR experiments using 1H, 13C, and 31P in various combinations. The 3-O-acetylglycerol moiety is substoichiometrically O-acetylated in ST11A; yet, key connectivities that unequivocally show O-acetylation at the glycerol are provided by the long-range correlations from the acetate methyl groups to the glycerol in the 1H-13C HMBC spectrum. Additionally, we clarify the 1H-31P assignments previously presented. © 2009 Elsevier Ltd. All rights reserved.
  • Authors

    Digital Object Identifier (doi)

    Author List

  • Zartler ER; Porambo RJ; Anderson CL; Yu J; Nahm MH
  • Start Page

  • 2586
  • End Page

  • 2590
  • Volume

  • 344
  • Issue

  • 18