A simple in situ cyanogen bromide cleavage method to obtain internal amino acid sequence of proteins electroblotted to polyvinyldifluoride membranes

Academic Article

Abstract

  • We report a simple method to obtain internal amino acid sequences from larger proteins electroblotted to polyvinyldifluoride membranes. To demonstrate this method, immunoglobulin heavy and light chains are separated by gel electrophoresis and electroblotted to the membrane. The separated chains, immobilized to the membrane, are cleaved in situ by cyanogen bromide and the resulting fragments are subsequently eluted from the membrane. The fragments are separated by gel electrophoresis, electroblotted and subjected to gas phase microsequence analysis. © 1988 Academic Press, Inc.
  • Authors

    Digital Object Identifier (doi)

    Author List

  • Scott MG; Crimmins DL; McCourt DW; Tarrand JJ; Eyerman MC; Nahm MH
  • Start Page

  • 1353
  • End Page

  • 1359
  • Volume

  • 155
  • Issue

  • 3