New fluorescence assay for the quantitation of fungi

Academic Article

Abstract

  • Quantitative determination of fungal mass is easily achieved with a new procedure that detects particle epifluorescence. Fungi are detected after exposure to a fluorescent stain (Fungiqual; CIBA-GEIGY Corp., Summit, N.J.) by using a fluorescence particle concentration analyzer. This report describes a simple fluorescence method for quantitation of either yeast or mycelial forms of fungi. The nature of the staining reaction was studied, and a practical application of this procedure for determination of fungal susceptibility to an antifungal agent is presented.
  • Authors

    Author List

  • Coleman T; Madassery JV; Kobayashi GS; Nahm MH; Little JR
  • Start Page

  • 2003
  • End Page

  • 2007
  • Volume

  • 27
  • Issue

  • 9