Use of HL-60 cell line to measure opsonic capacity of pneumococcal antibodies

Academic Article

Abstract

  • From a practical point of view, a single source for the HL-60 cell line is needed, as well as standardized culture and differentiation conditions. Suggested conditions for a standardized OPKA protocol are available online (64). This site is updated periodically. Despite confusing experiences in the past, the HL-60 cell line has been extensively characterized and is readily available; its use has been described for the evaluation of pneumococcal vaccine induced anti-capsular antibodies by standardized OPKA, and it remains a good candidate cell line. Perhaps a single distribution center for the cell line may be established to help the pneumococcal research community. In the meantime, any laboratory interested in OPA must obtain the correct HL-60 cell line from a qualified source (e.g., ATCC).
  • Authors

    Digital Object Identifier (doi)

    Author List

  • Fleck RA; Romero-Steiner S; Nahm MH
  • Start Page

  • 19
  • End Page

  • 27
  • Volume

  • 12
  • Issue

  • 1