Distribution of receptors for insulin and insulin-like growth factor I (somatomedin C) in the adrenal gland.

Academic Article

Abstract

  • Rat adrenal glands contain cell surface high-affinity receptors for several peptide hormones. Receptors for IGF-I were abundant in this tissue, but receptors for insulin were relatively scarce. The behavior of adrenal membrane IGF-I receptors in radioligand binding assays was similar to the behavior of IGF-I receptors from other tissues, with a KD congruent to 6.2 x 10(-9) M. Covalent cross-linking studies with [125I]IGF-I revealed an IGF-I receptor alpha-subunit with Mr congruent to 135,000 on dodecyl sulfate polyacrylamide gel electrophoresis under reducing conditions, as well as a smaller radiolabeled peptide, Mr = 116,000. In contrast, little binding of [125I]insulin to adrenal membranes was observed and no labeling occurred in cross-linking studies using [125I]insulin. These results contrast with the findings of whole-body autoradiographic studies that indicated substantial binding of [125I]insulin to adrenal glands and suggest that IGF-I, rather than insulin, may play a critical role in the growth and development of the adrenal gland.
  • Keywords

  • Adrenal Glands, Animals, Cell Membrane, Insulin, Insulin-Like Growth Factor I, Kinetics, Male, Rats, Rats, Inbred F344, Receptor, Insulin, Receptors, Somatomedin, Somatomedins
  • Author List

  • Pillion DJ; Yang M; Grizzle WE
  • Start Page

  • 138
  • End Page

  • 145
  • Volume

  • 154
  • Issue

  • 1