Coordinated but physically separable interaction with H3K27-demethylase and H3K4-methyltransferase activities are required for T-box protein-mediated activation of developmental gene expression

Academic Article

Abstract

  • During cellular differentiation, both permissive and repressive epigenetic modifications must be negotiated to create cell-type-specific gene expression patterns. The T-box transcription factor family is important in numerous developmental systems ranging from embryogenesis to the differentiation of adult tissues. By analyzing point mutations in conserved sequences in the T-box DNA-binding domain, we found that two overlapping, but physically separable regions are required for the physical and functional interaction with H3K27-demethylase and H3K4-methyltransferase activities. Importantly, the ability to associate with these histone-modifying complexes is a conserved function for the T-box family. These novel mechanisms for T-box-mediated epigenetic regulation are essential, because point mutations that disrupt these interactions are found in a diverse array of human developmental genetic diseases. © 2008 by Cold Spring Harbor Laboratory Press.
  • Published In

    Digital Object Identifier (doi)

    Author List

  • Miller SA; Huang AC; Miazgowicz MM; Brassil MM; Weinmann AS
  • Start Page

  • 2980
  • End Page

  • 2993
  • Volume

  • 22
  • Issue

  • 21