Purification, crystallization and X-ray analysis of Hibiscus chlorotic ringspot virus

Academic Article

Abstract

  • Hibiscus chlorotic ringspot virus (HCRSV), a Carmovirus, occurs worldwide and induces chlorotic ringspots on leaves, stunting and flower distortion in Hibiscus species, including kenaf. The HCRSV capsid has T = 3 icosahedral symmetry and contains 180 copies of the coat protein. A virus yield of 48-70 mg per 100 g of infected kenaf leaves was achieved with an improved purification scheme involving sucrose-cushion and sucrose density-gradient centrifugation. The virus was crystallized using PEG 8000 and 2,3-butanediol as co-precipitants. The crystals belonged to the cubic space group P23, with unit-cell parameter a = 392 Å, and diffracted X-rays to at least 4.5 Å resolution.
  • Authors

    Digital Object Identifier (doi)

    Author List

  • Lee KC; Lim D; Wong SM; Dokland T
  • Start Page

  • 1481
  • End Page

  • 1483
  • Volume

  • 59
  • Issue

  • 8